Drug discovery from natural sources including plants, marine organisms and endophytes is still pivotal in pharmaceutical research due to their ability of producing diverse and unique secondary metabolites. Discovery of secondary metabolites from leaves of Vitex pinnata, a medicinal plant from Malaysia and its associated endophytic fungi, Lasiodiplodia theobromae, Nigrospora sp and Pestalotiopsis olivacea afforded nineteen compounds and several of these compounds exhibited good anticancer, antitrypanosomal and anti-mycobacterial activities. Secondary metabolites isolated from leaves of V. pinnata previously reported to exhibit good anti-inflammatory activity and interestingly the leaves were used by local Malay community to treat cuts and wounds. In recent years new strategy using metabolomics in natural products is trending among scientists and found to be efficient, intelligent and robust. Implements of metabolomics including hyphenated HR-LCMS, NMR and integrated with in-house database, AntiMarin were utilised to exploit endophytic fungal natural products from V. pinnata. Preliminary exploration of secondary metabolites production of all three endophytic fungi was simply achieved by applying metabolomics strategy for medium-scale fermentation. Metabolomics also was used as decision-making strategy in mining active metabolomes of endophytic fungus, Lasiodiplodia theobromae against Trypanosoma brucei brucei. Dereplication approach using metabolomics for identification of compounds from Pestalotiopsis olivacea was easily accomplished.
|Date of Award||6 Oct 2015|
- University Of Strathclyde
|Supervisor||Ruangelie Edrada-Ebel (Supervisor) & Alexander Gray (Supervisor)|