3D biofabrication of constructs for orthopaedic tissue regeneration and clinical biofilm study

  • Gareth Stephen Turnbull

Student thesis: Doctoral Thesis

Abstract

The rising prevalence of arthritis and osteochondral defects (OCDs) has led to growing worldwide demand for joint replacement surgery. Meanwhile, the prevalence of antimicrobial resistance (AMR) and prosthetic joint infections (PJIs) in clinical orthopaedics is growing at alarming pace. A novel approach to help tackle AC defects and AMR alike is offered by 3D biofabrication, as a technology with the capacity to deposit cells, bacteria and biomaterials in user-defined patterns to build 3D constructs from the “bottom-up.” Initially composite AlgMA/Col and AlgMA/GelMA bioinks were developed and combined with a novel triple-crosslinking approach (double ionic and UV) following extrusion FRESH (freeform reversible embedding of suspended hydrogels) bioprinting to allow creation of 1mm thick constructs. Constructs maintained shape in culture over 28 days, whilst stability was improved with addition of AlgMA (p95%) with accelerated cell growth demonstrated with inclusion of cell spheroids (p
Date of Award5 Oct 2022
Original languageEnglish
Awarding Institution
  • University Of Strathclyde
SupervisorWill Shu (Supervisor) & Phil Riches (Supervisor)

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