Abstract
| Language | English |
|---|---|
| Article number | 0147115 |
| Number of pages | 19 |
| Journal | PLOS One |
| Volume | 11 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - 29 Jan 2016 |
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Keywords
- two-photon
- microscopy
- femtosecond
- Ca2+
- hippocampal
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Widefield two-photon excitation without scanning : live cell microscopy with high time resolution and low photo-bleaching. / Amor, Rumelo; McDonald, Alison; Trägårdh, Johanna; Robb, Gillian; Wilson, Louise; Abdul Rahman, Nor Zaihana; Dempster, John; Amos, William Bradshaw; Bushell, Trevor J.; McConnell, Gail.
In: PLOS One, Vol. 11, No. 1, 0147115, 29.01.2016.Research output: Contribution to journal › Article
TY - JOUR
T1 - Widefield two-photon excitation without scanning
T2 - PLOS One
AU - Amor, Rumelo
AU - McDonald, Alison
AU - Trägårdh, Johanna
AU - Robb, Gillian
AU - Wilson, Louise
AU - Abdul Rahman, Nor Zaihana
AU - Dempster, John
AU - Amos, William Bradshaw
AU - Bushell, Trevor J.
AU - McConnell, Gail
PY - 2016/1/29
Y1 - 2016/1/29
N2 - We demonstrate fluorescence imaging by two-photon excitation without scanning in biological specimens as previously described by Hwang and co-workers, but with an increased field size and with framing rates of up to 100 Hz. During recordings of synaptically-driven Ca2+ events in primary rat hippocampal neurone cultures loaded with the fluorescent Ca2+ indicator Fluo-4 AM, we have observed greatly reduced photo-bleaching in comparison with single-photon excitation. This method, which requires no costly additions to the microscope, promises to be useful for work where high time-resolution is required.
AB - We demonstrate fluorescence imaging by two-photon excitation without scanning in biological specimens as previously described by Hwang and co-workers, but with an increased field size and with framing rates of up to 100 Hz. During recordings of synaptically-driven Ca2+ events in primary rat hippocampal neurone cultures loaded with the fluorescent Ca2+ indicator Fluo-4 AM, we have observed greatly reduced photo-bleaching in comparison with single-photon excitation. This method, which requires no costly additions to the microscope, promises to be useful for work where high time-resolution is required.
KW - two-photon
KW - microscopy
KW - femtosecond
KW - Ca2+
KW - hippocampal
UR - http://www.plosone.org/
U2 - 10.1371/journal.pone.0147115
DO - 10.1371/journal.pone.0147115
M3 - Article
VL - 11
JO - PLOS One
JF - PLOS One
SN - 1932-6203
IS - 1
M1 - 0147115
ER -