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Abstract
We demonstrate fluorescence imaging by two-photon excitation without scanning in biological specimens as previously described by Hwang and co-workers, but with an increased field size and with framing rates of up to 100 Hz. During recordings of synaptically-driven Ca2+ events in primary rat hippocampal neurone cultures loaded with the fluorescent Ca2+ indicator Fluo-4 AM, we have observed greatly reduced photo-bleaching in comparison with single-photon excitation. This method, which requires no costly additions to the microscope, promises to be useful for work where high time-resolution is required.
Original language | English |
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Article number | 0147115 |
Number of pages | 19 |
Journal | PLOS One |
Volume | 11 |
Issue number | 1 |
DOIs | |
Publication status | Published - 29 Jan 2016 |
Keywords
- two-photon
- microscopy
- femtosecond
- Ca2+
- hippocampal
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Widefield two-photon excitation without scanning: live cell microscopy with high time resolution and low photo-bleaching
McConnell, G. (Creator), University of Strathclyde, 13 Jan 2016
DOI: 10.15129/7cd1c87c-f787-44ac-8333-0ab47e77f6cf
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