Widefield standing wave microscopy of red blood cell membrane morphology with high temporal resolution

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Abstract

We report the first demonstration of widefield standing wave (SW) microscopy of
fluorescently labelled red blood cells at high speeds which allows for rapid imaging of membrane deformations. Using existing and custom MATLAB functions, we also present a method to generate 2D and 3D reconstructions of the SW data for improved visualization of the cell. We compare our technique with standard widefield epifluorescence imaging and show that the SW technique not only reveals more topographical information about the specimen but does so without increasing toxicity or the rate of photobleaching could make this a powerful technique for the diagnosis or study of red blood cell morphology and biomechanical characteristics.
Original languageEnglish
Pages (from-to)1745-1761
Number of pages17
JournalBiomedical Optics Express
Volume9
Issue number4
DOIs
Publication statusPublished - 16 Mar 2018

Keywords

  • widefield standing wave
  • red blood cells
  • membrane deformation
  • epifluorescence imaging
  • fluorescence microscopy

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