Widefield standing wave microscopy of red blood cell membrane morphology with high temporal resolution

Research output: Contribution to journalArticle

Abstract

We report the first demonstration of widefield standing wave (SW) microscopy of
fluorescently labelled red blood cells at high speeds which allows for rapid imaging of membrane deformations. Using existing and custom MATLAB functions, we also present a method to generate 2D and 3D reconstructions of the SW data for improved visualization of the cell. We compare our technique with standard widefield epifluorescence imaging and show that the SW technique not only reveals more topographical information about the specimen but does so without increasing toxicity or the rate of photobleaching could make this a powerful technique for the diagnosis or study of red blood cell morphology and biomechanical characteristics.
LanguageEnglish
Pages1745-1761
Number of pages17
JournalBiomedical Optics Express
Volume9
Issue number4
DOIs
StatePublished - 16 Mar 2018

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erythrocytes
Cell membranes
temporal resolution
standing waves
Microscopy
Microscopic examination
Blood
Erythrocytes
Cell Membrane
microscopy
Photobleaching
high resolution
Cells
Imaging techniques
toxicity
MATLAB
Membranes
Toxicity
Demonstrations
Visualization

Keywords

  • widefield standing wave
  • red blood cells
  • membrane deformation
  • epifluorescence imaging
  • fluorescence microscopy

Cite this

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title = "Widefield standing wave microscopy of red blood cell membrane morphology with high temporal resolution",
abstract = "We report the first demonstration of widefield standing wave (SW) microscopy offluorescently labelled red blood cells at high speeds which allows for rapid imaging of membrane deformations. Using existing and custom MATLAB functions, we also present a method to generate 2D and 3D reconstructions of the SW data for improved visualization of the cell. We compare our technique with standard widefield epifluorescence imaging and show that the SW technique not only reveals more topographical information about the specimen but does so without increasing toxicity or the rate of photobleaching could make this a powerful technique for the diagnosis or study of red blood cell morphology and biomechanical characteristics.",
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AU - Scrimgeour,Ross

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N2 - We report the first demonstration of widefield standing wave (SW) microscopy offluorescently labelled red blood cells at high speeds which allows for rapid imaging of membrane deformations. Using existing and custom MATLAB functions, we also present a method to generate 2D and 3D reconstructions of the SW data for improved visualization of the cell. We compare our technique with standard widefield epifluorescence imaging and show that the SW technique not only reveals more topographical information about the specimen but does so without increasing toxicity or the rate of photobleaching could make this a powerful technique for the diagnosis or study of red blood cell morphology and biomechanical characteristics.

AB - We report the first demonstration of widefield standing wave (SW) microscopy offluorescently labelled red blood cells at high speeds which allows for rapid imaging of membrane deformations. Using existing and custom MATLAB functions, we also present a method to generate 2D and 3D reconstructions of the SW data for improved visualization of the cell. We compare our technique with standard widefield epifluorescence imaging and show that the SW technique not only reveals more topographical information about the specimen but does so without increasing toxicity or the rate of photobleaching could make this a powerful technique for the diagnosis or study of red blood cell morphology and biomechanical characteristics.

KW - widefield standing wave

KW - red blood cells

KW - membrane deformation

KW - epifluorescence imaging

KW - fluorescence microscopy

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