Wide-field multiphoton imaging through scattering media without correction

Adrià Escobet-Montalbán, Roman Spesyvtsev, Mingzhou Chen, Wardiya Afshar Saber, Melissa Andrews, C. Simon Herrington, Michael Mazilu, Kishan Dholakia

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Optical approaches to fluorescent, spectroscopic, and morphological imaging have made exceptional advances in the last decade. Super-resolution imaging and wide-field multiphoton imaging are now underpinning major advances across the biomedical sciences. While the advances have been startling, the key unmet challenge to date in all forms of optical imaging is to penetrate deeper. A number of schemes implement aberration correction or the use of complex photonics to address this need. In contrast, we approach this challenge by implementing a scheme that requires no a priori information about the medium nor its properties. Exploiting temporal focusing and single-pixel detection in our innovative scheme, we obtain wide-field two-photon images through various turbid media including a scattering phantom and tissue reaching a depth of up to seven scattering mean free path lengths. Our results show that it competes favorably with standard point-scanning two-photon imaging, with up to a fivefold improvement in signal-to-background ratio while showing significantly lower photobleaching.
LanguageEnglish
Number of pages9
JournalScience Advances
Volume4
Issue number10
DOIs
Publication statusPublished - 2 Oct 2018

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photons
scattering
mean free path
aberration
pixels
photonics
scanning

Keywords

  • imaging
  • microscopy
  • compressive sensing

Cite this

Escobet-Montalbán, A., Spesyvtsev, R., Chen, M., Saber, W. A., Andrews, M., Herrington, C. S., ... Dholakia, K. (2018). Wide-field multiphoton imaging through scattering media without correction. Science Advances, 4(10). https://doi.org/10.1126/sciadv.aau1338
Escobet-Montalbán, Adrià ; Spesyvtsev, Roman ; Chen, Mingzhou ; Saber, Wardiya Afshar ; Andrews, Melissa ; Herrington, C. Simon ; Mazilu, Michael ; Dholakia, Kishan. / Wide-field multiphoton imaging through scattering media without correction. In: Science Advances. 2018 ; Vol. 4, No. 10.
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Escobet-Montalbán, A, Spesyvtsev, R, Chen, M, Saber, WA, Andrews, M, Herrington, CS, Mazilu, M & Dholakia, K 2018, 'Wide-field multiphoton imaging through scattering media without correction' Science Advances, vol. 4, no. 10. https://doi.org/10.1126/sciadv.aau1338

Wide-field multiphoton imaging through scattering media without correction. / Escobet-Montalbán, Adrià ; Spesyvtsev, Roman; Chen, Mingzhou; Saber, Wardiya Afshar; Andrews, Melissa; Herrington, C. Simon ; Mazilu, Michael; Dholakia, Kishan.

In: Science Advances, Vol. 4, No. 10, 02.10.2018.

Research output: Contribution to journalArticle

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AU - Escobet-Montalbán, Adrià

AU - Spesyvtsev, Roman

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AU - Andrews, Melissa

AU - Herrington, C. Simon

AU - Mazilu, Michael

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Escobet-Montalbán A, Spesyvtsev R, Chen M, Saber WA, Andrews M, Herrington CS et al. Wide-field multiphoton imaging through scattering media without correction. Science Advances. 2018 Oct 2;4(10). https://doi.org/10.1126/sciadv.aau1338