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Stefanie Griesbeck, Zuolun Zhang, Marcus Gutmann, Tessa Lühmann, Robert M. Edkins, Guillaume Clermont, Adina N. Lazar, Martin Haehnel, Katharina Edkins, Antonius Eichhorn, Mireille Blanchard-Desce*, Lorenz Meinel, Todd B. Marder
Research output: Contribution to journal › Article › peer-review
Three water-soluble tetracationic quadrupolar chromophores comprising two three-coordinate boron π-acceptor groups bridged by thiophene-containing moieties were synthesised for biological imaging applications. Compound 3 containing the bulkier 5-(3,5-Me2C6H2)-2,2′-(C4H2S)2-5′-(3,5-Me2C6H2) bridge is stable over a long period of time, exhibits a high fluorescence quantum yield and strong one- and two-photon absorption (TPA), and has a TPA cross section of 268 GM at 800 nm in water. Confocal laser scanning fluorescence microscopy studies in live cells indicated localisation of the chromophore at the mitochondria; moreover, cytotoxicity measurements proved biocompatibility. Thus, chromophore 3 has excellent potential for one- and two-photon-excited fluorescence imaging of mitochondrial function in cells.
Original language | English |
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Pages (from-to) | 14701-14706 |
Number of pages | 6 |
Journal | Chemistry - A European Journal |
Volume | 22 |
Issue number | 41 |
Early online date | 15 Sept 2016 |
DOIs | |
Publication status | Published - 4 Oct 2016 |
T.B.M. is grateful for generous financial support from the Bavarian State Ministry of Science, Research, and the Arts for the Collaborative Research Network ?Solar Technologies go Hybrid?. Z.Z. and R.M.E. thank the Alexander von Humboldt Foundation for Postdoctoral Research Fellowships. R.M.E also thanks The Royal Commission for the Exhibition of 1851 for a research fellowship. M.B.D. is grateful for generous funding from the R?gion Aquitaine (chair of excellence). This study has also been carried out with financial support from the French State, managed by the French National Research Agency (ANR) in the frame of ?the Investments for the future? Programme IdEx Bordeaux - LAPHIA (ANR-10-IDEX-03-02). The two-photon microscopy was carried out at the Bordeaux Imaging Center, a service unit of the CNRS-INSERM and Bordeaux University, a member of the national infrastructure France BioImaging. Financial support by Deutsche Forschungsgemeinschaft (DFG; ME-3820/3-1) is gratefully acknowledged by L.M.
Research output: Contribution to journal › Article › peer-review