Virulent rough filaments of Listeria monocytogenes from clinical and food samples secreting wild-type levels of cell-free p60 protein

Neil J. Rowan; Alan A. G. Candlish; Andreas Bubert; John G. Anderson; Karl Kramer; Jim McLauchlin

doi:10.1128/jcm.38.7.2643-2648.2000

Virulent rough filaments of Listeria monocytogenes from clinical and food samples secreting wild-type levels of cell-free p60 protein

Neil J. Rowan^*, Alan A. G. Candlish, Andreas Bubert, John G. Anderson, Karl Kramer, Jim McLauchlin

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

37 Citations (Scopus)

Abstract

Atypical rough cell filaments of Listeria monocytogenes (designated FR variants), isolated from clinical and food samples, form long filaments up to 96 μm in length and demonstrated wild-type levels of adherence, invasion, and cytotoxicity to human epithelial HEp-2, Caco-2, and HeLa cells. Unlike previously described avirulent rough mutants of L. monocytogenes that secrete diminished levels of the major extracellular protein p60 and that form long chains that consist of multiple cells of similar size (designated MCR variants), FR variants secreted wild-type or greater levels of p60. This study shows that virulent filamentous forms of L. monocytogenes occur in clinical and food environments and have atypical morphological characteristics compared to those of the wild-type form.

Original language	English
Pages (from-to)	2643-2648
Number of pages	6
Journal	Journal of Clinical Microbiology
Volume	38
Issue number	7
DOIs	https://doi.org/10.1128/jcm.38.7.2643-2648.2000
Publication status	Published - 1 Jul 2000

Keywords

Listeria monocytogenes
p60 protein
food-borne infections

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10.1128/jcm.38.7.2643-2648.2000

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title = "Virulent rough filaments of Listeria monocytogenes from clinical and food samples secreting wild-type levels of cell-free p60 protein",

abstract = "Atypical rough cell filaments of Listeria monocytogenes (designated FR variants), isolated from clinical and food samples, form long filaments up to 96 μm in length and demonstrated wild-type levels of adherence, invasion, and cytotoxicity to human epithelial HEp-2, Caco-2, and HeLa cells. Unlike previously described avirulent rough mutants of L. monocytogenes that secrete diminished levels of the major extracellular protein p60 and that form long chains that consist of multiple cells of similar size (designated MCR variants), FR variants secreted wild-type or greater levels of p60. This study shows that virulent filamentous forms of L. monocytogenes occur in clinical and food environments and have atypical morphological characteristics compared to those of the wild-type form.",

keywords = "Listeria monocytogenes, p60 protein, food-borne infections",

author = "Rowan, {Neil J.} and Candlish, {Alan A. G.} and Andreas Bubert and Anderson, {John G.} and Karl Kramer and Jim McLauchlin",

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TY - JOUR

T1 - Virulent rough filaments of Listeria monocytogenes from clinical and food samples secreting wild-type levels of cell-free p60 protein

AU - Rowan, Neil J.

AU - Candlish, Alan A. G.

AU - Bubert, Andreas

AU - Anderson, John G.

AU - Kramer, Karl

AU - McLauchlin, Jim

PY - 2000/7/1

Y1 - 2000/7/1

N2 - Atypical rough cell filaments of Listeria monocytogenes (designated FR variants), isolated from clinical and food samples, form long filaments up to 96 μm in length and demonstrated wild-type levels of adherence, invasion, and cytotoxicity to human epithelial HEp-2, Caco-2, and HeLa cells. Unlike previously described avirulent rough mutants of L. monocytogenes that secrete diminished levels of the major extracellular protein p60 and that form long chains that consist of multiple cells of similar size (designated MCR variants), FR variants secreted wild-type or greater levels of p60. This study shows that virulent filamentous forms of L. monocytogenes occur in clinical and food environments and have atypical morphological characteristics compared to those of the wild-type form.

AB - Atypical rough cell filaments of Listeria monocytogenes (designated FR variants), isolated from clinical and food samples, form long filaments up to 96 μm in length and demonstrated wild-type levels of adherence, invasion, and cytotoxicity to human epithelial HEp-2, Caco-2, and HeLa cells. Unlike previously described avirulent rough mutants of L. monocytogenes that secrete diminished levels of the major extracellular protein p60 and that form long chains that consist of multiple cells of similar size (designated MCR variants), FR variants secreted wild-type or greater levels of p60. This study shows that virulent filamentous forms of L. monocytogenes occur in clinical and food environments and have atypical morphological characteristics compared to those of the wild-type form.

KW - Listeria monocytogenes

KW - p60 protein

KW - food-borne infections

U2 - 10.1128/jcm.38.7.2643-2648.2000

DO - 10.1128/jcm.38.7.2643-2648.2000

M3 - Article

C2 - 10878057

AN - SCOPUS:0033915414

SN - 0095-1137

VL - 38

SP - 2643

EP - 2648

JO - Journal of Clinical Microbiology

JF - Journal of Clinical Microbiology

IS - 7

ER -

Virulent rough filaments of Listeria monocytogenes from clinical and food samples secreting wild-type levels of cell-free p60 protein

Abstract

Keywords

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