Vibrational spectroscopic methods for cytology and cellular research

Graeme Clemens; James R. Hands; Konrad M. Dorling; Matthew J. Baker

doi:10.1039/c4an00636d

Vibrational spectroscopic methods for cytology and cellular research

Graeme Clemens^*, James R. Hands, Konrad M. Dorling, Matthew J. Baker

^*Corresponding author for this work

Pure And Applied Chemistry

Research output: Contribution to journal › Article › peer-review

90 Citations (Scopus)

Abstract

The use of vibrational spectroscopy, FTIR and Raman, for cytology and cellular research has the potential to revolutionise the approach to cellular analysis. Vibrational spectroscopy is non-destructive, simple to operate and provides direct information. Importantly it does not require expensive exogenous labels that may affect the chemistry of the cell under analysis. In addition, the advent of spectroscopic microscopes provides the ability to image cells and acquire spectra with a subcellular resolution. This introductory review focuses on recent developments within this fast paced field and highlights potential for the future use of FTIR and Raman spectroscopy. We particularly focus on the development of live cell research and the new technologies and methodologies that have enabled this.

Original language	English
Pages (from-to)	4411-4444
Number of pages	34
Journal	Analyst
Volume	139
Issue number	18
Early online date	27 Jun 2014
DOIs	https://doi.org/10.1039/c4an00636d
Publication status	Published - 21 Sept 2014

Keywords

vibrational spectroscopy
cytology
cellular research

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10.1039/c4an00636d

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AB - The use of vibrational spectroscopy, FTIR and Raman, for cytology and cellular research has the potential to revolutionise the approach to cellular analysis. Vibrational spectroscopy is non-destructive, simple to operate and provides direct information. Importantly it does not require expensive exogenous labels that may affect the chemistry of the cell under analysis. In addition, the advent of spectroscopic microscopes provides the ability to image cells and acquire spectra with a subcellular resolution. This introductory review focuses on recent developments within this fast paced field and highlights potential for the future use of FTIR and Raman spectroscopy. We particularly focus on the development of live cell research and the new technologies and methodologies that have enabled this.

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Vibrational spectroscopic methods for cytology and cellular research

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Keywords

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