Vascular prostheses: performance related to cell-shear responses

K.D. Andrews, P. Feugier, R.A. Black, J.A. Hunt

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

This work concerned the endothelialization of vascular prostheses and subsequent improvement of functionality with respect to tissue engineering. The aim of the study was to investigate the initial, pre-shear stress cellular behavior with respect to three vascular biomaterials to explain subsequent cellular responses to physiological shear stresses. All three vascular biomaterials demonstrated different structures. Cell behavior varied both between the materials and the two cell types: cytoskeletal involvement was greater for the HUVECs and the more fibrous surfaces; height profiles were greater for the L929 and PET, and lowest on PU. Immunohistochemistry of HUVEC samples also showed differences: PU revealed the greatest expression of intercellular adhesion molecule-1 and E-selectin (PET and ePTFE the lowest, respectively); ePTFE produced the greatest for vascular cell adhesion molecule-1 (PET the lowest). Material substrate influenced the cellular response. Cells demonstrating firm adhesion increased their cytoskeletal processes and expression of cell-substratum and inter-cellular adhesion markers, which may explain their ability to adapt more readily to shear stress. The fibrous PU structure appeared to be most suited to further shear stress exposure. This study demonstrated the potential of the underlying vascular material to affect the long-term cellular functionality of the prosthesis.
Original languageEnglish
Pages (from-to)39-46
Number of pages8
JournalJournal of Surgical Research
Volume149
Issue number1
DOIs
Publication statusPublished - Sep 2008

Fingerprint

Blood Vessel Prosthesis
Blood Vessels
Biocompatible Materials
E-Selectin
Vascular Cell Adhesion Molecule-1
Intercellular Adhesion Molecule-1
Tissue Engineering
Prostheses and Implants
Immunohistochemistry

Keywords

  • tissue engineering
  • blood vessel prosthesis
  • cell responses
  • electrospinning
  • endothelial cells
  • regional blood flow
  • static culture
  • shear stress

Cite this

Andrews, K.D. ; Feugier, P. ; Black, R.A. ; Hunt, J.A. / Vascular prostheses : performance related to cell-shear responses. In: Journal of Surgical Research. 2008 ; Vol. 149, No. 1. pp. 39-46.
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Vascular prostheses : performance related to cell-shear responses. / Andrews, K.D.; Feugier, P.; Black, R.A.; Hunt, J.A.

In: Journal of Surgical Research, Vol. 149, No. 1, 09.2008, p. 39-46.

Research output: Contribution to journalArticle

TY - JOUR

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T2 - performance related to cell-shear responses

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AU - Feugier, P.

AU - Black, R.A.

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AB - This work concerned the endothelialization of vascular prostheses and subsequent improvement of functionality with respect to tissue engineering. The aim of the study was to investigate the initial, pre-shear stress cellular behavior with respect to three vascular biomaterials to explain subsequent cellular responses to physiological shear stresses. All three vascular biomaterials demonstrated different structures. Cell behavior varied both between the materials and the two cell types: cytoskeletal involvement was greater for the HUVECs and the more fibrous surfaces; height profiles were greater for the L929 and PET, and lowest on PU. Immunohistochemistry of HUVEC samples also showed differences: PU revealed the greatest expression of intercellular adhesion molecule-1 and E-selectin (PET and ePTFE the lowest, respectively); ePTFE produced the greatest for vascular cell adhesion molecule-1 (PET the lowest). Material substrate influenced the cellular response. Cells demonstrating firm adhesion increased their cytoskeletal processes and expression of cell-substratum and inter-cellular adhesion markers, which may explain their ability to adapt more readily to shear stress. The fibrous PU structure appeared to be most suited to further shear stress exposure. This study demonstrated the potential of the underlying vascular material to affect the long-term cellular functionality of the prosthesis.

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KW - cell responses

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