Variability of invitro migration of human-leukocytes

J.D. Pattison, D. Filer, W.G. Anderson, G. Gettinby, J.M. Anderson

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

A comparison of the capillary method (CM) and the agarose microdroplet method (AMM), using the same materials, for measurement of human leucocyte migration in vitro yielded similar large degrees of variability. Between fifteen and twenty-six replicate cultures were established for each method in fourteen subjects The range of the coefficients of variation were .7-28.8% (CM) and 7.2-19.3% AMM). Most of 115 publications reviewed describe only three or four replicates. Their results relating to host responses in the fields of cancer, tissue transplantation and several chronic diseases should be interpreted with regard to the variability of the system. The use of human leucocyte migration in vitro for any purpose should incorporate more stringent statistical control of sample size than has been customary. The use of two standard statistical formulae is proposed as this control.
LanguageEnglish
Pages191-194
Number of pages3
JournalJournal of Clinical and Laboratory Immunology
Volume5
Issue number3
Publication statusPublished - May 1981

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Leukocytes
Sepharose
Tissue Transplantation
Sample Size
Publications
Chronic Disease
Neoplasms
In Vitro Techniques

Keywords

  • capillary method
  • agarose microdroplet method
  • comparison
  • materials
  • measurement
  • human leucocyte migration
  • in vitro
  • variability

Cite this

Pattison, J. D., Filer, D., Anderson, W. G., Gettinby, G., & Anderson, J. M. (1981). Variability of invitro migration of human-leukocytes. Journal of Clinical and Laboratory Immunology, 5(3), 191-194.
Pattison, J.D. ; Filer, D. ; Anderson, W.G. ; Gettinby, G. ; Anderson, J.M. / Variability of invitro migration of human-leukocytes. In: Journal of Clinical and Laboratory Immunology. 1981 ; Vol. 5, No. 3. pp. 191-194.
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Pattison, JD, Filer, D, Anderson, WG, Gettinby, G & Anderson, JM 1981, 'Variability of invitro migration of human-leukocytes' Journal of Clinical and Laboratory Immunology, vol. 5, no. 3, pp. 191-194.

Variability of invitro migration of human-leukocytes. / Pattison, J.D.; Filer, D.; Anderson, W.G.; Gettinby, G.; Anderson, J.M.

In: Journal of Clinical and Laboratory Immunology, Vol. 5, No. 3, 05.1981, p. 191-194.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Variability of invitro migration of human-leukocytes

AU - Pattison, J.D.

AU - Filer, D.

AU - Anderson, W.G.

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AB - A comparison of the capillary method (CM) and the agarose microdroplet method (AMM), using the same materials, for measurement of human leucocyte migration in vitro yielded similar large degrees of variability. Between fifteen and twenty-six replicate cultures were established for each method in fourteen subjects The range of the coefficients of variation were .7-28.8% (CM) and 7.2-19.3% AMM). Most of 115 publications reviewed describe only three or four replicates. Their results relating to host responses in the fields of cancer, tissue transplantation and several chronic diseases should be interpreted with regard to the variability of the system. The use of human leucocyte migration in vitro for any purpose should incorporate more stringent statistical control of sample size than has been customary. The use of two standard statistical formulae is proposed as this control.

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Pattison JD, Filer D, Anderson WG, Gettinby G, Anderson JM. Variability of invitro migration of human-leukocytes. Journal of Clinical and Laboratory Immunology. 1981 May;5(3):191-194.