Utilisation of an electrochemiluminescence sensor for atropine determination in complex matrices

Research output: Contribution to journalArticle

Abstract

A major challenge within forensic science is the development of accurate and robust methodologies that can be utilized on-site for detection at crime scenes and can be used for analyzing multiple sample types. The recent expansion of electrochemical sensors to tackle this hurdle requires sensors that can undergo analysis without any pretreatment. Given the vast array of samples that are submitted for forensic analysis, this can pose a major challenge for all electrochemical sensors, including electrochemiluminescent (ECL)-based sensors. Within this contribution, we demonstrate the capacity for an ECL-based sensor to address this challenge and it is potential to detect and quantify atropine from a wide range of samples directly from herbal material to spiked solutions. This portable platform demonstrates satisfactory analytical parameters with linearity across a concentration range of 0.75 to 100 μM, reproducibility of 3.0%, repeatability of 9.2%, and a detection limit of ∼0.75 μM. The sensor displays good selectivity toward alkaloid species and, in particular, the hallucinogenic tropane alkaloid functionality within complex matrices. This portable sensor provides rapid detection alongside low cost and operational simplicity, thus, providing a basis for the exploitation of ECL-based sensors within the forensic arena.

LanguageEnglish
Pages12369-12376
Number of pages8
JournalAnalytical Chemistry
Volume91
Issue number19
Early online date22 Aug 2019
DOIs
Publication statusPublished - 1 Oct 2019

Fingerprint

Atropine
Sensors
Electrochemical sensors
Alkaloids
Tropanes
Crime
Costs

Keywords

  • electrochemical sensors
  • electrochemiluminescent based sensors
  • ECL
  • portable platform

Cite this

@article{58456bb0eefc42b8ab79fb0df2d65dd1,
title = "Utilisation of an electrochemiluminescence sensor for atropine determination in complex matrices",
abstract = "A major challenge within forensic science is the development of accurate and robust methodologies that can be utilized on-site for detection at crime scenes and can be used for analyzing multiple sample types. The recent expansion of electrochemical sensors to tackle this hurdle requires sensors that can undergo analysis without any pretreatment. Given the vast array of samples that are submitted for forensic analysis, this can pose a major challenge for all electrochemical sensors, including electrochemiluminescent (ECL)-based sensors. Within this contribution, we demonstrate the capacity for an ECL-based sensor to address this challenge and it is potential to detect and quantify atropine from a wide range of samples directly from herbal material to spiked solutions. This portable platform demonstrates satisfactory analytical parameters with linearity across a concentration range of 0.75 to 100 μM, reproducibility of 3.0{\%}, repeatability of 9.2{\%}, and a detection limit of ∼0.75 μM. The sensor displays good selectivity toward alkaloid species and, in particular, the hallucinogenic tropane alkaloid functionality within complex matrices. This portable sensor provides rapid detection alongside low cost and operational simplicity, thus, providing a basis for the exploitation of ECL-based sensors within the forensic arena.",
keywords = "electrochemical sensors, electrochemiluminescent based sensors, ECL, portable platform",
author = "Kelly Brown and Moira McMenemy and Matthew Palmer and Baker, {Matthew J.} and Robinson, {David W.} and Pamela Allan and Lynn Dennany",
year = "2019",
month = "10",
day = "1",
doi = "10.1021/acs.analchem.9b02905",
language = "English",
volume = "91",
pages = "12369--12376",
journal = "Analytical Chemistry",
issn = "0003-2700",
publisher = "American Chemical Society",
number = "19",

}

Utilisation of an electrochemiluminescence sensor for atropine determination in complex matrices. / Brown, Kelly; McMenemy, Moira; Palmer, Matthew ; Baker, Matthew J.; Robinson, David W.; Allan, Pamela; Dennany, Lynn.

In: Analytical Chemistry, Vol. 91, No. 19, 01.10.2019, p. 12369-12376.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Utilisation of an electrochemiluminescence sensor for atropine determination in complex matrices

AU - Brown, Kelly

AU - McMenemy, Moira

AU - Palmer, Matthew

AU - Baker, Matthew J.

AU - Robinson, David W.

AU - Allan, Pamela

AU - Dennany, Lynn

PY - 2019/10/1

Y1 - 2019/10/1

N2 - A major challenge within forensic science is the development of accurate and robust methodologies that can be utilized on-site for detection at crime scenes and can be used for analyzing multiple sample types. The recent expansion of electrochemical sensors to tackle this hurdle requires sensors that can undergo analysis without any pretreatment. Given the vast array of samples that are submitted for forensic analysis, this can pose a major challenge for all electrochemical sensors, including electrochemiluminescent (ECL)-based sensors. Within this contribution, we demonstrate the capacity for an ECL-based sensor to address this challenge and it is potential to detect and quantify atropine from a wide range of samples directly from herbal material to spiked solutions. This portable platform demonstrates satisfactory analytical parameters with linearity across a concentration range of 0.75 to 100 μM, reproducibility of 3.0%, repeatability of 9.2%, and a detection limit of ∼0.75 μM. The sensor displays good selectivity toward alkaloid species and, in particular, the hallucinogenic tropane alkaloid functionality within complex matrices. This portable sensor provides rapid detection alongside low cost and operational simplicity, thus, providing a basis for the exploitation of ECL-based sensors within the forensic arena.

AB - A major challenge within forensic science is the development of accurate and robust methodologies that can be utilized on-site for detection at crime scenes and can be used for analyzing multiple sample types. The recent expansion of electrochemical sensors to tackle this hurdle requires sensors that can undergo analysis without any pretreatment. Given the vast array of samples that are submitted for forensic analysis, this can pose a major challenge for all electrochemical sensors, including electrochemiluminescent (ECL)-based sensors. Within this contribution, we demonstrate the capacity for an ECL-based sensor to address this challenge and it is potential to detect and quantify atropine from a wide range of samples directly from herbal material to spiked solutions. This portable platform demonstrates satisfactory analytical parameters with linearity across a concentration range of 0.75 to 100 μM, reproducibility of 3.0%, repeatability of 9.2%, and a detection limit of ∼0.75 μM. The sensor displays good selectivity toward alkaloid species and, in particular, the hallucinogenic tropane alkaloid functionality within complex matrices. This portable sensor provides rapid detection alongside low cost and operational simplicity, thus, providing a basis for the exploitation of ECL-based sensors within the forensic arena.

KW - electrochemical sensors

KW - electrochemiluminescent based sensors

KW - ECL

KW - portable platform

U2 - 10.1021/acs.analchem.9b02905

DO - 10.1021/acs.analchem.9b02905

M3 - Article

VL - 91

SP - 12369

EP - 12376

JO - Analytical Chemistry

T2 - Analytical Chemistry

JF - Analytical Chemistry

SN - 0003-2700

IS - 19

ER -