Using two photon microscopy to quantify enzymatic reaction rates on polymer beads

A.Y. Bosman, R.V. Ulijn, G. McConnell, J.M. Girkin, P.J. Halling, S.L. Flitsch

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Two-photon fluorescence microscopy was introduced as a tool to assess enzyme accessibility and to quantify enzyme reactions rates on solid supports. Enzyme catalysis on substrates that are linked to solid supports (solid phase biocatalysis) is becoming increasingly important as polymer supported synthesis1 and high throughput screening methods2 are developed. Despite successes both in synthesis and analysis, fundamental understanding of the kinetics and thermodynamics of such enzyme catalysed reactions is limited. It is increasingly clear that the fundamental rules for solid phase chemistry are different from those of solution phase chemistry,3 and the same can be expected for solid phase biocatalysis. These rules need to be better understood in order to exploit the advantages of solid phase reactions in full. Hence, we recently initiated research efforts aimed at a better fundamental understanding of solid phase biocatalysis.
LanguageEnglish
Pages2790-2791
Number of pages2
JournalChemical Communications (London)
Volume2003
Issue number23
Early online date13 Oct 2003
DOIs
Publication statusPublished - 13 Oct 2003

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Reaction rates
Microscopic examination
Polymers
Photons
Enzymes
Catalyst supports
Fluorescence microscopy
Catalysis
Screening
Throughput
Thermodynamics
Kinetics
Substrates
Biocatalysis

Keywords

  • solid-support
  • peptide-synthesis
  • confocal raman
  • pega supports
  • chemistry
  • kinetics
  • library

Cite this

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title = "Using two photon microscopy to quantify enzymatic reaction rates on polymer beads",
abstract = "Two-photon fluorescence microscopy was introduced as a tool to assess enzyme accessibility and to quantify enzyme reactions rates on solid supports. Enzyme catalysis on substrates that are linked to solid supports (solid phase biocatalysis) is becoming increasingly important as polymer supported synthesis1 and high throughput screening methods2 are developed. Despite successes both in synthesis and analysis, fundamental understanding of the kinetics and thermodynamics of such enzyme catalysed reactions is limited. It is increasingly clear that the fundamental rules for solid phase chemistry are different from those of solution phase chemistry,3 and the same can be expected for solid phase biocatalysis. These rules need to be better understood in order to exploit the advantages of solid phase reactions in full. Hence, we recently initiated research efforts aimed at a better fundamental understanding of solid phase biocatalysis.",
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Using two photon microscopy to quantify enzymatic reaction rates on polymer beads. / Bosman, A.Y.; Ulijn, R.V.; McConnell, G.; Girkin, J.M.; Halling, P.J.; Flitsch, S.L.

In: Chemical Communications (London), Vol. 2003, No. 23, 13.10.2003, p. 2790-2791.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Using two photon microscopy to quantify enzymatic reaction rates on polymer beads

AU - Bosman, A.Y.

AU - Ulijn, R.V.

AU - McConnell, G.

AU - Girkin, J.M.

AU - Halling, P.J.

AU - Flitsch, S.L.

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AB - Two-photon fluorescence microscopy was introduced as a tool to assess enzyme accessibility and to quantify enzyme reactions rates on solid supports. Enzyme catalysis on substrates that are linked to solid supports (solid phase biocatalysis) is becoming increasingly important as polymer supported synthesis1 and high throughput screening methods2 are developed. Despite successes both in synthesis and analysis, fundamental understanding of the kinetics and thermodynamics of such enzyme catalysed reactions is limited. It is increasingly clear that the fundamental rules for solid phase chemistry are different from those of solution phase chemistry,3 and the same can be expected for solid phase biocatalysis. These rules need to be better understood in order to exploit the advantages of solid phase reactions in full. Hence, we recently initiated research efforts aimed at a better fundamental understanding of solid phase biocatalysis.

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KW - peptide-synthesis

KW - confocal raman

KW - pega supports

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