Abstract
| Language | English |
|---|---|
| Pages | 2790-2791 |
| Number of pages | 2 |
| Journal | Chemical Communications (London) |
| Volume | 2003 |
| Issue number | 23 |
| Early online date | 13 Oct 2003 |
| DOIs | |
| Publication status | Published - 13 Oct 2003 |
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Keywords
- solid-support
- peptide-synthesis
- confocal raman
- pega supports
- chemistry
- kinetics
- library
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Using two photon microscopy to quantify enzymatic reaction rates on polymer beads. / Bosman, A.Y.; Ulijn, R.V.; McConnell, G.; Girkin, J.M.; Halling, P.J.; Flitsch, S.L.
In: Chemical Communications (London), Vol. 2003, No. 23, 13.10.2003, p. 2790-2791.Research output: Contribution to journal › Article
TY - JOUR
T1 - Using two photon microscopy to quantify enzymatic reaction rates on polymer beads
AU - Bosman, A.Y.
AU - Ulijn, R.V.
AU - McConnell, G.
AU - Girkin, J.M.
AU - Halling, P.J.
AU - Flitsch, S.L.
N1 - © The Royal Society of Chemistry 2003
PY - 2003/10/13
Y1 - 2003/10/13
N2 - Two-photon fluorescence microscopy was introduced as a tool to assess enzyme accessibility and to quantify enzyme reactions rates on solid supports. Enzyme catalysis on substrates that are linked to solid supports (solid phase biocatalysis) is becoming increasingly important as polymer supported synthesis1 and high throughput screening methods2 are developed. Despite successes both in synthesis and analysis, fundamental understanding of the kinetics and thermodynamics of such enzyme catalysed reactions is limited. It is increasingly clear that the fundamental rules for solid phase chemistry are different from those of solution phase chemistry,3 and the same can be expected for solid phase biocatalysis. These rules need to be better understood in order to exploit the advantages of solid phase reactions in full. Hence, we recently initiated research efforts aimed at a better fundamental understanding of solid phase biocatalysis.
AB - Two-photon fluorescence microscopy was introduced as a tool to assess enzyme accessibility and to quantify enzyme reactions rates on solid supports. Enzyme catalysis on substrates that are linked to solid supports (solid phase biocatalysis) is becoming increasingly important as polymer supported synthesis1 and high throughput screening methods2 are developed. Despite successes both in synthesis and analysis, fundamental understanding of the kinetics and thermodynamics of such enzyme catalysed reactions is limited. It is increasingly clear that the fundamental rules for solid phase chemistry are different from those of solution phase chemistry,3 and the same can be expected for solid phase biocatalysis. These rules need to be better understood in order to exploit the advantages of solid phase reactions in full. Hence, we recently initiated research efforts aimed at a better fundamental understanding of solid phase biocatalysis.
KW - solid-support
KW - peptide-synthesis
KW - confocal raman
KW - pega supports
KW - chemistry
KW - kinetics
KW - library
U2 - 10.1039/b308078a
DO - 10.1039/b308078a
M3 - Article
VL - 2003
SP - 2790
EP - 2791
JO - Chemical Communications (London)
T2 - Chemical Communications (London)
JF - Chemical Communications (London)
SN - 0009-241X
IS - 23
ER -