Using the Taguchi method for rapid quantitative PCR optimization with SYBR Green I

Phuvadol Thanakiatkrai, Lindsey Welch

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Here, we applied the Taguchi method, an engineering optimization process, to successfully determine the optimal conditions for three SYBR Green I-based quantitative PCR assays. This method balanced the effects of all factors and their associated levels by using an orthogonal array rather than a factorial array. Instead of running 27 experiments with the conventional factorial method, the Taguchi method achieved the same optimal conditions using only nine experiments, saving valuable resources.
LanguageEnglish
Pages161-165
Number of pages5
JournalInternational Journal of Legal Medicine
Volume126
Issue number1
DOIs
Publication statusPublished - 2012

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Polymerase Chain Reaction
SYBR Green I

Keywords

  • real-time PCR
  • SYBR Green I
  • factorial
  • qPCR
  • DNA amplification
  • Taguchi
  • optimization
  • orthogonal

Cite this

Thanakiatkrai, Phuvadol ; Welch, Lindsey. / Using the Taguchi method for rapid quantitative PCR optimization with SYBR Green I. In: International Journal of Legal Medicine. 2012 ; Vol. 126, No. 1. pp. 161-165.
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Using the Taguchi method for rapid quantitative PCR optimization with SYBR Green I. / Thanakiatkrai, Phuvadol; Welch, Lindsey.

In: International Journal of Legal Medicine, Vol. 126, No. 1, 2012, p. 161-165.

Research output: Contribution to journalArticle

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