Here, we applied the Taguchi method, an engineering optimization process, to successfully determine the optimal conditions for three SYBR Green I-based quantitative PCR assays. This method balanced the effects of all factors and their associated levels by using an orthogonal array rather than a factorial array. Instead of running 27 experiments with the conventional factorial method, the Taguchi method achieved the same optimal conditions using only nine experiments, saving valuable resources.
- real-time PCR
- SYBR Green I
- DNA amplification
Thanakiatkrai, P., & Welch, L. (2012). Using the Taguchi method for rapid quantitative PCR optimization with SYBR Green I. International Journal of Legal Medicine, 126(1), 161-165. https://doi.org/10.1007/s00414-011-0558-5