Using the Mesolens to observe structural changes in E. coli mature colony biofilms under different nutrient availability

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Abstract

The morphology of bacterial colonies depends on nutrient availability . We use the Mesolens , which can image a 100 mm 3 volume with subcellular resolution throughout, to investigate the internal structure of colonies grown on agar substrates with different glucose and ammonia concentrations. We observe intra
colony channels in both nutrient deprived and nutrient rich
colonies, with channel widths dependent on the nutrient
availability.
Original languageEnglish
Publication statusPublished - 25 May 2021
EventSUPA Virtual Gathering -
Duration: 25 May 202127 May 2021

Conference

ConferenceSUPA Virtual Gathering
Period25/05/2127/05/21

Keywords

  • microscopy
  • microbiology
  • biofilms
  • Mesolens
  • E. coli - Escherichia coli

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