Using 3D printed optical elements for multifocal image scanning microscopy

Jay Christopher; Mark Donnachie; Liam Rooney; Gail McConnell; Deepak Uttamchandani; Ralf Bauer

doi:10.1117/12.3002507

Using 3D printed optical elements for multifocal image scanning microscopy

Jay Christopher^*, Mark Donnachie, Liam Rooney, Gail McConnell, Deepak Uttamchandani, Ralf Bauer

^*Corresponding author for this work

Research output: Contribution to journal › Conference article › peer-review

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Abstract

We present a small footprint and affordable implementation of a multifocal image scanning microscope (ISM), utilizing both a microelectromechanical system (MEMS) micromirror for flexible optical excitation control, and multifocal patterned illumination using a custom 3D printed optical quality lenslet array. We highlight the individual element performance and demonstrate its use in fluorescence imaging to allow comparison of the affordable and customizable approach with its commercial counterpart.

Original language	English
Article number	1282702
Journal	Proceedings of SPIE
Volume	12827
DOIs	https://doi.org/10.1117/12.3002507
Publication status	Published - 12 Mar 2024
Event	SPIE BiOS 2024 - The Moscone Center, San Francisco, United States Duration: 27 Jan 2024 → 1 Feb 2024 https://spie.org/conferences-and-exhibitions/photonics-west

Keywords

3D printing
3D printed optics
structured illumination
mSIM
image scanning microscopy
ISM

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10.1117/12.3002507

Christopher-etal-SPIE-2024-Using-3D-printed-optical-elements-for-multifocal-image-scanning-microscopy
For the purposes of open access, a CC BY licence has been applied to this accepted manuscript
Accepted author manuscript, 1.3 MBLicence: CC BY 4.0

Cite this

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title = "Using 3D printed optical elements for multifocal image scanning microscopy",

abstract = "We present a small footprint and affordable implementation of a multifocal image scanning microscope (ISM), utilizing both a microelectromechanical system (MEMS) micromirror for flexible optical excitation control, and multifocal patterned illumination using a custom 3D printed optical quality lenslet array. We highlight the individual element performance and demonstrate its use in fluorescence imaging to allow comparison of the affordable and customizable approach with its commercial counterpart.",

keywords = "3D printing, 3D printed optics, structured illumination, mSIM, image scanning microscopy, ISM",

author = "Jay Christopher and Mark Donnachie and Liam Rooney and Gail McConnell and Deepak Uttamchandani and Ralf Bauer",

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doi = "10.1117/12.3002507",

language = "English",

volume = "12827",

journal = "Proceedings of SPIE",

issn = "0277-786X",

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TY - JOUR

T1 - Using 3D printed optical elements for multifocal image scanning microscopy

AU - Christopher, Jay

AU - Donnachie, Mark

AU - Rooney, Liam

AU - McConnell, Gail

AU - Uttamchandani, Deepak

AU - Bauer, Ralf

PY - 2024/3/12

Y1 - 2024/3/12

N2 - We present a small footprint and affordable implementation of a multifocal image scanning microscope (ISM), utilizing both a microelectromechanical system (MEMS) micromirror for flexible optical excitation control, and multifocal patterned illumination using a custom 3D printed optical quality lenslet array. We highlight the individual element performance and demonstrate its use in fluorescence imaging to allow comparison of the affordable and customizable approach with its commercial counterpart.

AB - We present a small footprint and affordable implementation of a multifocal image scanning microscope (ISM), utilizing both a microelectromechanical system (MEMS) micromirror for flexible optical excitation control, and multifocal patterned illumination using a custom 3D printed optical quality lenslet array. We highlight the individual element performance and demonstrate its use in fluorescence imaging to allow comparison of the affordable and customizable approach with its commercial counterpart.

KW - 3D printing

KW - 3D printed optics

KW - structured illumination

KW - mSIM

KW - image scanning microscopy

KW - ISM

UR - https://doi.org/10.17868/strath.00089844

U2 - 10.1117/12.3002507

DO - 10.1117/12.3002507

M3 - Conference article

SN - 0277-786X

VL - 12827

JO - Proceedings of SPIE

JF - Proceedings of SPIE

M1 - 1282702

T2 - SPIE BiOS 2024

Y2 - 27 January 2024 through 1 February 2024

ER -

Using 3D printed optical elements for multifocal image scanning microscopy

Abstract

Keywords

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Doctoral Training Partnership 2020-2021 University of Strathclyde | Christopher, Jay

MEMS-enabled miniaturised multimodal microscopy through pulsed structured illumination

Miniaturised 3D-printed biomedical imaging system using optical MEMS - RAE Fellowships (GCRF)

Best Student Paper Award

Cite this

Using 3D printed optical elements for multifocal image scanning microscopy

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Projects

Doctoral Training Partnership 2020-2021 University of Strathclyde | Christopher, Jay

MEMS-enabled miniaturised multimodal microscopy through pulsed structured illumination

Miniaturised 3D-printed biomedical imaging system using optical MEMS - RAE Fellowships (GCRF)

Prizes

Best Student Paper Award

Cite this