Use of the skin sandwich technique to probe the role of the hair follicles in sonophoresis

O. Sarheed, Y. Frum

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

The human skin sandwich technique was used to explore the effect of brief ultrasound exposure on the transfollicular pathway of absorption. Hydrocortisone was used as a model drug. In order to calculate the permeability coefficient of hydrocortisone, its concentration at saturation in the PBS donor solution was determined. Skin samples were prepared by sandwich technique with total hydration of the epidermal and sandwich membranes. The skin was sonicated for 0 s (control), 30 s or 45 s using a pulsed mode (10% duty cycle) with the spatial and temporal average intensity (SATA) of 3.7 W/cm2. The transducer was then removed and permeation was allowed to proceed for 52 h. Then the percentage follicular contribution was determined. It was determined that without ultrasound, drug entry into follicles accounted for 46% of total penetration. As the duration of sonication increased, the follicular contribution fell to zero even though total transepidermal flux dramatically increased. This is explained by ultrasound exposure causing sloughing off of the uppermost stratum corneum. This permeabilises the continuous surface but at the same time the disturbed cornceocytes will plug hair follicle orifices
Original languageEnglish
Pages (from-to)179-183
Number of pages5
JournalInternational Journal of Pharmaceutics
Volume423
Issue number2
DOIs
Publication statusPublished - 28 Feb 2012

Fingerprint

Hair Follicle
Skin
Hydrocortisone
Sonication
Transducers
Pharmaceutical Preparations
Cornea
Permeability
Membranes

Keywords

  • topically applied substances
  • hair follicles
  • permeation
  • diffusion
  • low frequency ultrasound
  • shunt route penetration
  • sonophoresis
  • percutaneous-absorption
  • transfollicular drug-delivery
  • follicular penetration
  • human scalp skin
  • skin sandwich technique
  • probe

Cite this

@article{5afeaa83208e44fdb057cb83c0afee4c,
title = "Use of the skin sandwich technique to probe the role of the hair follicles in sonophoresis",
abstract = "The human skin sandwich technique was used to explore the effect of brief ultrasound exposure on the transfollicular pathway of absorption. Hydrocortisone was used as a model drug. In order to calculate the permeability coefficient of hydrocortisone, its concentration at saturation in the PBS donor solution was determined. Skin samples were prepared by sandwich technique with total hydration of the epidermal and sandwich membranes. The skin was sonicated for 0 s (control), 30 s or 45 s using a pulsed mode (10{\%} duty cycle) with the spatial and temporal average intensity (SATA) of 3.7 W/cm2. The transducer was then removed and permeation was allowed to proceed for 52 h. Then the percentage follicular contribution was determined. It was determined that without ultrasound, drug entry into follicles accounted for 46{\%} of total penetration. As the duration of sonication increased, the follicular contribution fell to zero even though total transepidermal flux dramatically increased. This is explained by ultrasound exposure causing sloughing off of the uppermost stratum corneum. This permeabilises the continuous surface but at the same time the disturbed cornceocytes will plug hair follicle orifices",
keywords = "topically applied substances, hair follicles, permeation, diffusion, low frequency ultrasound, shunt route penetration, sonophoresis, percutaneous-absorption, transfollicular drug-delivery, follicular penetration, human scalp skin, skin sandwich technique, probe",
author = "O. Sarheed and Y. Frum",
year = "2012",
month = "2",
day = "28",
doi = "10.1016/j.ijpharm.2011.12.023",
language = "English",
volume = "423",
pages = "179--183",
journal = "International Journal of Pharmaceutics",
issn = "0378-5173",
number = "2",

}

Use of the skin sandwich technique to probe the role of the hair follicles in sonophoresis. / Sarheed, O.; Frum, Y.

In: International Journal of Pharmaceutics, Vol. 423, No. 2, 28.02.2012, p. 179-183.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Use of the skin sandwich technique to probe the role of the hair follicles in sonophoresis

AU - Sarheed, O.

AU - Frum, Y.

PY - 2012/2/28

Y1 - 2012/2/28

N2 - The human skin sandwich technique was used to explore the effect of brief ultrasound exposure on the transfollicular pathway of absorption. Hydrocortisone was used as a model drug. In order to calculate the permeability coefficient of hydrocortisone, its concentration at saturation in the PBS donor solution was determined. Skin samples were prepared by sandwich technique with total hydration of the epidermal and sandwich membranes. The skin was sonicated for 0 s (control), 30 s or 45 s using a pulsed mode (10% duty cycle) with the spatial and temporal average intensity (SATA) of 3.7 W/cm2. The transducer was then removed and permeation was allowed to proceed for 52 h. Then the percentage follicular contribution was determined. It was determined that without ultrasound, drug entry into follicles accounted for 46% of total penetration. As the duration of sonication increased, the follicular contribution fell to zero even though total transepidermal flux dramatically increased. This is explained by ultrasound exposure causing sloughing off of the uppermost stratum corneum. This permeabilises the continuous surface but at the same time the disturbed cornceocytes will plug hair follicle orifices

AB - The human skin sandwich technique was used to explore the effect of brief ultrasound exposure on the transfollicular pathway of absorption. Hydrocortisone was used as a model drug. In order to calculate the permeability coefficient of hydrocortisone, its concentration at saturation in the PBS donor solution was determined. Skin samples were prepared by sandwich technique with total hydration of the epidermal and sandwich membranes. The skin was sonicated for 0 s (control), 30 s or 45 s using a pulsed mode (10% duty cycle) with the spatial and temporal average intensity (SATA) of 3.7 W/cm2. The transducer was then removed and permeation was allowed to proceed for 52 h. Then the percentage follicular contribution was determined. It was determined that without ultrasound, drug entry into follicles accounted for 46% of total penetration. As the duration of sonication increased, the follicular contribution fell to zero even though total transepidermal flux dramatically increased. This is explained by ultrasound exposure causing sloughing off of the uppermost stratum corneum. This permeabilises the continuous surface but at the same time the disturbed cornceocytes will plug hair follicle orifices

KW - topically applied substances

KW - hair follicles

KW - permeation

KW - diffusion

KW - low frequency ultrasound

KW - shunt route penetration

KW - sonophoresis

KW - percutaneous-absorption

KW - transfollicular drug-delivery

KW - follicular penetration

KW - human scalp skin

KW - skin sandwich technique

KW - probe

UR - http://www.scopus.com/inward/record.url?scp=84856547093&partnerID=8YFLogxK

U2 - 10.1016/j.ijpharm.2011.12.023

DO - 10.1016/j.ijpharm.2011.12.023

M3 - Article

VL - 423

SP - 179

EP - 183

JO - International Journal of Pharmaceutics

JF - International Journal of Pharmaceutics

SN - 0378-5173

IS - 2

ER -