Use of optimisation algorithmic techniques with active optics for aberration correction in optical sectioning microscopy

S.P. Poland, D. Burns, W. Lubeigt, B.A. Patterson, G.J. Valentine, A.J. Wright, J.M. Girkin, Wenhan Jiang (Editor)

Research output: Chapter in Book/Report/Conference proceedingChapter

2 Citations (Scopus)

Abstract

Confocal techniques allow the user to achieve optically sectioned images with significantly enhanced axial and improved lateral resolution compared to widefield methods. Unfortunately, as one images more deeply within a sample, sample induced aberrations lead to a significant reduction in image resolution and contrast. Using adaptive optic techniques, we report on the effectiveness of a number of algorithms for removing sample induced aberrations. The viability and efficiency at a number of fitness parameters used in the optimisation routines is also considered.
LanguageEnglish
Title of host publication5th International Workshop on Adaptive Optics for Industry and Medicine Proceedings
Volume6018
DOIs
Publication statusPublished - 21 Dec 2005

Fingerprint

aberration
optics
microscopy
optimization
fitness
image resolution
image contrast
adaptive optics
viability

Keywords

  • algorithmic techniques
  • active optics
  • aberration correction
  • microscopy

Cite this

Poland, S. P., Burns, D., Lubeigt, W., Patterson, B. A., Valentine, G. J., Wright, A. J., ... Jiang, W. (Ed.) (2005). Use of optimisation algorithmic techniques with active optics for aberration correction in optical sectioning microscopy. In 5th International Workshop on Adaptive Optics for Industry and Medicine Proceedings (Vol. 6018) https://doi.org/10.1117/12.669388
Poland, S.P. ; Burns, D. ; Lubeigt, W. ; Patterson, B.A. ; Valentine, G.J. ; Wright, A.J. ; Girkin, J.M. ; Jiang, Wenhan (Editor). / Use of optimisation algorithmic techniques with active optics for aberration correction in optical sectioning microscopy. 5th International Workshop on Adaptive Optics for Industry and Medicine Proceedings. Vol. 6018 2005.
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Poland, SP, Burns, D, Lubeigt, W, Patterson, BA, Valentine, GJ, Wright, AJ, Girkin, JM & Jiang, W (ed.) 2005, Use of optimisation algorithmic techniques with active optics for aberration correction in optical sectioning microscopy. in 5th International Workshop on Adaptive Optics for Industry and Medicine Proceedings. vol. 6018. https://doi.org/10.1117/12.669388

Use of optimisation algorithmic techniques with active optics for aberration correction in optical sectioning microscopy. / Poland, S.P.; Burns, D.; Lubeigt, W.; Patterson, B.A.; Valentine, G.J.; Wright, A.J.; Girkin, J.M.; Jiang, Wenhan (Editor).

5th International Workshop on Adaptive Optics for Industry and Medicine Proceedings. Vol. 6018 2005.

Research output: Chapter in Book/Report/Conference proceedingChapter

TY - CHAP

T1 - Use of optimisation algorithmic techniques with active optics for aberration correction in optical sectioning microscopy

AU - Poland, S.P.

AU - Burns, D.

AU - Lubeigt, W.

AU - Patterson, B.A.

AU - Valentine, G.J.

AU - Wright, A.J.

AU - Girkin, J.M.

A2 - Jiang, Wenhan

PY - 2005/12/21

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N2 - Confocal techniques allow the user to achieve optically sectioned images with significantly enhanced axial and improved lateral resolution compared to widefield methods. Unfortunately, as one images more deeply within a sample, sample induced aberrations lead to a significant reduction in image resolution and contrast. Using adaptive optic techniques, we report on the effectiveness of a number of algorithms for removing sample induced aberrations. The viability and efficiency at a number of fitness parameters used in the optimisation routines is also considered.

AB - Confocal techniques allow the user to achieve optically sectioned images with significantly enhanced axial and improved lateral resolution compared to widefield methods. Unfortunately, as one images more deeply within a sample, sample induced aberrations lead to a significant reduction in image resolution and contrast. Using adaptive optic techniques, we report on the effectiveness of a number of algorithms for removing sample induced aberrations. The viability and efficiency at a number of fitness parameters used in the optimisation routines is also considered.

KW - algorithmic techniques

KW - active optics

KW - aberration correction

KW - microscopy

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DO - 10.1117/12.669388

M3 - Chapter

SN - 9780819460424

VL - 6018

BT - 5th International Workshop on Adaptive Optics for Industry and Medicine Proceedings

ER -

Poland SP, Burns D, Lubeigt W, Patterson BA, Valentine GJ, Wright AJ et al. Use of optimisation algorithmic techniques with active optics for aberration correction in optical sectioning microscopy. In 5th International Workshop on Adaptive Optics for Industry and Medicine Proceedings. Vol. 6018. 2005 https://doi.org/10.1117/12.669388