Use of optimisation algorithmic techniques with active optics for aberration correction in optical sectioning microscopy

S.P. Poland; D. Burns; W. Lubeigt; B.A. Patterson; G.J. Valentine; A.J. Wright; J.M. Girkin; Wenhan Jiang

doi:10.1117/12.669388

Use of optimisation algorithmic techniques with active optics for aberration correction in optical sectioning microscopy

S.P. Poland, D. Burns, W. Lubeigt, B.A. Patterson, G.J. Valentine, A.J. Wright, J.M. Girkin, Wenhan Jiang (Editor)

Research output: Chapter in Book/Report/Conference proceeding › Chapter

2 Citations (Scopus)

Abstract

Confocal techniques allow the user to achieve optically sectioned images with significantly enhanced axial and improved lateral resolution compared to widefield methods. Unfortunately, as one images more deeply within a sample, sample induced aberrations lead to a significant reduction in image resolution and contrast. Using adaptive optic techniques, we report on the effectiveness of a number of algorithms for removing sample induced aberrations. The viability and efficiency at a number of fitness parameters used in the optimisation routines is also considered.

Original language	English
Title of host publication	5th International Workshop on Adaptive Optics for Industry and Medicine Proceedings
Volume	6018
DOIs	https://doi.org/10.1117/12.669388
Publication status	Published - 21 Dec 2005

Keywords

algorithmic techniques
active optics
aberration correction
microscopy

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10.1117/12.669388

http://dx.doi.org/10.1117/12.669388

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Poland, S. P., Burns, D., Lubeigt, W., Patterson, B. A., Valentine, G. J., Wright, A. J., Girkin, J. M., & Jiang, W. (Ed.) (2005). Use of optimisation algorithmic techniques with active optics for aberration correction in optical sectioning microscopy. In 5th International Workshop on Adaptive Optics for Industry and Medicine Proceedings (Vol. 6018) https://doi.org/10.1117/12.669388

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Use of optimisation algorithmic techniques with active optics for aberration correction in optical sectioning microscopy. / Poland, S.P.; Burns, D.; Lubeigt, W.; Patterson, B.A.; Valentine, G.J.; Wright, A.J.; Girkin, J.M.; Jiang, Wenhan (Editor).

5th International Workshop on Adaptive Optics for Industry and Medicine Proceedings. Vol. 6018 2005.

Research output: Chapter in Book/Report/Conference proceeding › Chapter

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AU - Lubeigt, W.

AU - Patterson, B.A.

AU - Valentine, G.J.

AU - Wright, A.J.

AU - Girkin, J.M.

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KW - active optics

KW - aberration correction

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