Use of optimisation algorithmic techniques with active optics for aberration correction in optical sectioning microscopy

S.P. Poland; D. Burns; W. Lubeigt; B.A. Patterson; G.J. Valentine; A.J. Wright; J.M. Girkin; Wenhan Jiang

doi:10.1117/12.669388

Use of optimisation algorithmic techniques with active optics for aberration correction in optical sectioning microscopy

S.P. Poland, D. Burns, W. Lubeigt, B.A. Patterson, G.J. Valentine, A.J. Wright, J.M. Girkin, Wenhan Jiang (Editor)

Research output: Chapter in Book/Report/Conference proceeding › Chapter

2 Citations (Scopus)

Abstract

Confocal techniques allow the user to achieve optically sectioned images with significantly enhanced axial and improved lateral resolution compared to widefield methods. Unfortunately, as one images more deeply within a sample, sample induced aberrations lead to a significant reduction in image resolution and contrast. Using adaptive optic techniques, we report on the effectiveness of a number of algorithms for removing sample induced aberrations. The viability and efficiency at a number of fitness parameters used in the optimisation routines is also considered.

Original language	English
Title of host publication	5th International Workshop on Adaptive Optics for Industry and Medicine Proceedings
Volume	6018
DOIs	https://doi.org/10.1117/12.669388
Publication status	Published - 21 Dec 2005

Keywords

algorithmic techniques
active optics
aberration correction
microscopy

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10.1117/12.669388

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Poland, S. P., Burns, D., Lubeigt, W., Patterson, B. A., Valentine, G. J., Wright, A. J., Girkin, J. M., & Jiang, W. (Ed.) (2005). Use of optimisation algorithmic techniques with active optics for aberration correction in optical sectioning microscopy. In 5th International Workshop on Adaptive Optics for Industry and Medicine Proceedings (Vol. 6018) https://doi.org/10.1117/12.669388

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abstract = "Confocal techniques allow the user to achieve optically sectioned images with significantly enhanced axial and improved lateral resolution compared to widefield methods. Unfortunately, as one images more deeply within a sample, sample induced aberrations lead to a significant reduction in image resolution and contrast. Using adaptive optic techniques, we report on the effectiveness of a number of algorithms for removing sample induced aberrations. The viability and efficiency at a number of fitness parameters used in the optimisation routines is also considered.",

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Use of optimisation algorithmic techniques with active optics for aberration correction in optical sectioning microscopy. / Poland, S.P.; Burns, D.; Lubeigt, W.; Patterson, B.A.; Valentine, G.J.; Wright, A.J.; Girkin, J.M.; Jiang, Wenhan (Editor).

5th International Workshop on Adaptive Optics for Industry and Medicine Proceedings. Vol. 6018 2005.

Research output: Chapter in Book/Report/Conference proceeding › Chapter

TY - CHAP

T1 - Use of optimisation algorithmic techniques with active optics for aberration correction in optical sectioning microscopy

AU - Poland, S.P.

AU - Burns, D.

AU - Lubeigt, W.

AU - Patterson, B.A.

AU - Valentine, G.J.

AU - Wright, A.J.

AU - Girkin, J.M.

A2 - Jiang, Wenhan

PY - 2005/12/21

Y1 - 2005/12/21

N2 - Confocal techniques allow the user to achieve optically sectioned images with significantly enhanced axial and improved lateral resolution compared to widefield methods. Unfortunately, as one images more deeply within a sample, sample induced aberrations lead to a significant reduction in image resolution and contrast. Using adaptive optic techniques, we report on the effectiveness of a number of algorithms for removing sample induced aberrations. The viability and efficiency at a number of fitness parameters used in the optimisation routines is also considered.

AB - Confocal techniques allow the user to achieve optically sectioned images with significantly enhanced axial and improved lateral resolution compared to widefield methods. Unfortunately, as one images more deeply within a sample, sample induced aberrations lead to a significant reduction in image resolution and contrast. Using adaptive optic techniques, we report on the effectiveness of a number of algorithms for removing sample induced aberrations. The viability and efficiency at a number of fitness parameters used in the optimisation routines is also considered.

KW - algorithmic techniques

KW - active optics

KW - aberration correction

KW - microscopy

UR - http://dx.doi.org/10.1117/12.669388

U2 - 10.1117/12.669388

DO - 10.1117/12.669388

M3 - Chapter

SN - 9780819460424

VL - 6018

BT - 5th International Workshop on Adaptive Optics for Industry and Medicine Proceedings

ER -

Use of optimisation algorithmic techniques with active optics for aberration correction in optical sectioning microscopy

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