Confocal techniques allow the user to achieve optically sectioned images with significantly enhanced axial and improved lateral resolution compared to widefield methods. Unfortunately, as one images more deeply within a sample, sample induced aberrations lead to a significant reduction in image resolution and contrast. Using adaptive optic techniques, we report on the effectiveness of a number of algorithms for removing sample induced aberrations. The viability and efficiency at a number of fitness parameters used in the optimisation routines is also considered.
|Title of host publication||5th International Workshop on Adaptive Optics for Industry and Medicine Proceedings|
|Publication status||Published - 21 Dec 2005|
- algorithmic techniques
- active optics
- aberration correction