Use of adaptive optics for improved multiphoton imaging

J.M. Girkin, P.N. Marsh

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

We report on the integration of active optical elements in a multiphoton microscope to improve theresolution and overall image quality when imaging deeply into biological samples. Optical models were generated of sample systems and these have been compared with the performance of thecomplete imaging system. The active elements used were commercially available flexible membranemirrors controlled by custom, home written, software. Significant improvements in image qualityhave been demonstrated using a range of optimisation routines based on the analysis of the imagesproduced by the system, rather than with a wavefront sensor. A three-fold increase in the resolution100µm into the sample was achieved.
LanguageEnglish
Pages260
Number of pages7
JournalProceedings of SPIE: The International Society for Optical Engineering
Volume5323
DOIs
Publication statusPublished - 2004

Fingerprint

Adaptive optics
Adaptive Optics
Wavefronts
Optical devices
adaptive optics
Imaging systems
Image quality
Microscopes
Imaging
Imaging techniques
Sensors
Wavefront Sensor
Threefolds
Imaging System
Microscope
Image Quality
microscopes
computer programs
optimization
Software

Keywords

  • multiphoton imaging
  • adaptive optics
  • microscopy
  • deep tissue imaging
  • spherical-aberration
  • confocal microscopy
  • 2-photon microscope
  • compensation

Cite this

@article{091fd0c2c4da4e11b2430913919f9cfe,
title = "Use of adaptive optics for improved multiphoton imaging",
abstract = "We report on the integration of active optical elements in a multiphoton microscope to improve theresolution and overall image quality when imaging deeply into biological samples. Optical models were generated of sample systems and these have been compared with the performance of thecomplete imaging system. The active elements used were commercially available flexible membranemirrors controlled by custom, home written, software. Significant improvements in image qualityhave been demonstrated using a range of optimisation routines based on the analysis of the imagesproduced by the system, rather than with a wavefront sensor. A three-fold increase in the resolution100µm into the sample was achieved.",
keywords = "multiphoton imaging, adaptive optics, microscopy, deep tissue imaging, spherical-aberration, confocal microscopy, 2-photon microscope, compensation",
author = "J.M. Girkin and P.N. Marsh",
year = "2004",
doi = "10.1117/12.525659",
language = "English",
volume = "5323",
pages = "260",
journal = "Proceedings of SPIE",
issn = "0277-786X",

}

Use of adaptive optics for improved multiphoton imaging. / Girkin, J.M.; Marsh, P.N.

In: Proceedings of SPIE: The International Society for Optical Engineering, Vol. 5323, 2004, p. 260.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Use of adaptive optics for improved multiphoton imaging

AU - Girkin, J.M.

AU - Marsh, P.N.

PY - 2004

Y1 - 2004

N2 - We report on the integration of active optical elements in a multiphoton microscope to improve theresolution and overall image quality when imaging deeply into biological samples. Optical models were generated of sample systems and these have been compared with the performance of thecomplete imaging system. The active elements used were commercially available flexible membranemirrors controlled by custom, home written, software. Significant improvements in image qualityhave been demonstrated using a range of optimisation routines based on the analysis of the imagesproduced by the system, rather than with a wavefront sensor. A three-fold increase in the resolution100µm into the sample was achieved.

AB - We report on the integration of active optical elements in a multiphoton microscope to improve theresolution and overall image quality when imaging deeply into biological samples. Optical models were generated of sample systems and these have been compared with the performance of thecomplete imaging system. The active elements used were commercially available flexible membranemirrors controlled by custom, home written, software. Significant improvements in image qualityhave been demonstrated using a range of optimisation routines based on the analysis of the imagesproduced by the system, rather than with a wavefront sensor. A three-fold increase in the resolution100µm into the sample was achieved.

KW - multiphoton imaging

KW - adaptive optics

KW - microscopy

KW - deep tissue imaging

KW - spherical-aberration

KW - confocal microscopy

KW - 2-photon microscope

KW - compensation

U2 - 10.1117/12.525659

DO - 10.1117/12.525659

M3 - Article

VL - 5323

SP - 260

JO - Proceedings of SPIE

T2 - Proceedings of SPIE

JF - Proceedings of SPIE

SN - 0277-786X

ER -