Abstract
| Language | English |
|---|---|
| Pages | 260 |
| Number of pages | 7 |
| Journal | Proceedings of SPIE: The International Society for Optical Engineering |
| Volume | 5323 |
| DOIs | |
| Publication status | Published - 2004 |
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Keywords
- multiphoton imaging
- adaptive optics
- microscopy
- deep tissue imaging
- spherical-aberration
- confocal microscopy
- 2-photon microscope
- compensation
Cite this
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Use of adaptive optics for improved multiphoton imaging. / Girkin, J.M.; Marsh, P.N.
In: Proceedings of SPIE: The International Society for Optical Engineering, Vol. 5323, 2004, p. 260.Research output: Contribution to journal › Article
TY - JOUR
T1 - Use of adaptive optics for improved multiphoton imaging
AU - Girkin, J.M.
AU - Marsh, P.N.
PY - 2004
Y1 - 2004
N2 - We report on the integration of active optical elements in a multiphoton microscope to improve theresolution and overall image quality when imaging deeply into biological samples. Optical models were generated of sample systems and these have been compared with the performance of thecomplete imaging system. The active elements used were commercially available flexible membranemirrors controlled by custom, home written, software. Significant improvements in image qualityhave been demonstrated using a range of optimisation routines based on the analysis of the imagesproduced by the system, rather than with a wavefront sensor. A three-fold increase in the resolution100µm into the sample was achieved.
AB - We report on the integration of active optical elements in a multiphoton microscope to improve theresolution and overall image quality when imaging deeply into biological samples. Optical models were generated of sample systems and these have been compared with the performance of thecomplete imaging system. The active elements used were commercially available flexible membranemirrors controlled by custom, home written, software. Significant improvements in image qualityhave been demonstrated using a range of optimisation routines based on the analysis of the imagesproduced by the system, rather than with a wavefront sensor. A three-fold increase in the resolution100µm into the sample was achieved.
KW - multiphoton imaging
KW - adaptive optics
KW - microscopy
KW - deep tissue imaging
KW - spherical-aberration
KW - confocal microscopy
KW - 2-photon microscope
KW - compensation
U2 - 10.1117/12.525659
DO - 10.1117/12.525659
M3 - Article
VL - 5323
SP - 260
JO - Proceedings of SPIE
T2 - Proceedings of SPIE
JF - Proceedings of SPIE
SN - 0277-786X
ER -