Uptake of Cr (VI) and Cr (III)-GSH complex across rat liver cell nuclei

V.K. Raghunathan, J.N.A. Tettey, M.H. Grant

Research output: Contribution to journalArticle

Abstract

Cr (VI) is a known carcinogen that readily crosses the cellular membrane and is then reduced by intracellular reductants such as reduced glutathione (GSH) to Cr (III), forming various reactive intermediate compounds (Ding and Shi, 2002). The interaction of Cr (VI) and GSH is known to be one of the causes of DNA damage in vitro (Mazzer et al., 2007). This study investigates the occurrence of Cr (III)-GSH in freshly isolated rat hepatocytes incubated with Cr (VI), and the uptake of Cr (VI) and Cr (III)-GSH complex across rat liver cell nuclei. Cr (III)-GSH complex was synthesised by the method of Levina et al. (2003), and its formation confirmed by liquid chromatography-mass spectrometry (LC-MS).
LanguageEnglish
Pages20-21
Number of pages1
JournalToxicology
Volume253
Issue number1-3
DOIs
Publication statusPublished - 2008

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Cell Nucleus
Liver
Rats
Cells
Reducing Agents
Liquid chromatography
Liquid Chromatography
Carcinogens
DNA Damage
Mass spectrometry
Glutathione
Hepatocytes
Mass Spectrometry
Membranes
chromium hexavalent ion
DNA

Keywords

  • toxicology
  • bioengineering
  • rat liver
  • rat hepatocytes
  • cell nuclei

Cite this

Raghunathan, V. K., Tettey, J. N. A., & Grant, M. H. (2008). Uptake of Cr (VI) and Cr (III)-GSH complex across rat liver cell nuclei. Toxicology, 253(1-3), 20-21. https://doi.org/10.1016/j.tox.2008.07.025
Raghunathan, V.K. ; Tettey, J.N.A. ; Grant, M.H. / Uptake of Cr (VI) and Cr (III)-GSH complex across rat liver cell nuclei. In: Toxicology. 2008 ; Vol. 253, No. 1-3. pp. 20-21.
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Raghunathan, VK, Tettey, JNA & Grant, MH 2008, 'Uptake of Cr (VI) and Cr (III)-GSH complex across rat liver cell nuclei' Toxicology, vol. 253, no. 1-3, pp. 20-21. https://doi.org/10.1016/j.tox.2008.07.025

Uptake of Cr (VI) and Cr (III)-GSH complex across rat liver cell nuclei. / Raghunathan, V.K.; Tettey, J.N.A.; Grant, M.H.

In: Toxicology, Vol. 253, No. 1-3, 2008, p. 20-21.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Uptake of Cr (VI) and Cr (III)-GSH complex across rat liver cell nuclei

AU - Raghunathan, V.K.

AU - Tettey, J.N.A.

AU - Grant, M.H.

PY - 2008

Y1 - 2008

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AB - Cr (VI) is a known carcinogen that readily crosses the cellular membrane and is then reduced by intracellular reductants such as reduced glutathione (GSH) to Cr (III), forming various reactive intermediate compounds (Ding and Shi, 2002). The interaction of Cr (VI) and GSH is known to be one of the causes of DNA damage in vitro (Mazzer et al., 2007). This study investigates the occurrence of Cr (III)-GSH in freshly isolated rat hepatocytes incubated with Cr (VI), and the uptake of Cr (VI) and Cr (III)-GSH complex across rat liver cell nuclei. Cr (III)-GSH complex was synthesised by the method of Levina et al. (2003), and its formation confirmed by liquid chromatography-mass spectrometry (LC-MS).

KW - toxicology

KW - bioengineering

KW - rat liver

KW - rat hepatocytes

KW - cell nuclei

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U2 - 10.1016/j.tox.2008.07.025

DO - 10.1016/j.tox.2008.07.025

M3 - Article

VL - 253

SP - 20

EP - 21

JO - Toxicology

T2 - Toxicology

JF - Toxicology

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