Uptake of chromium into red blood cells does not affect the ability of haemoglobin to bind oxygen

G.A. Afolaranmi, J.N.A. Tettey, J.D.S. Gaylor, H.M. Murray, R.M.D. Meek, M.H. Grant

Research output: Contribution to journalArticle

Abstract

Many of the metallic implants used in orthopaedics are made of stainless steel or cobalt-chromium alloys which contain 18-30% chromium. Hexavalent chromium (Cr VI) has been shown to be the predominant form of chromium released following in vivo and in vitro corrosion of these metal implants (Merritt and Brown, 1995 K. Merritt and S.A. Brown, J. Biomed. Mater. Res. 29 (1995), pp. 627-633. Full Text via CrossRef | View Record in Scopus | Cited By in Scopus (67)Merritt and Brown, 1995). Blood chromium levels may be elevated between 50 and 250 times in patients with metal hip implants (Lhotka et al., 2003). At physiological pH, Cr (VI) exists predominantly as the chromate anion and as such can enter cells via non-specific anion channels. The anionic Cr (VI) diffuses readily through the red blood cell (RBC) membrane and is bound by the haemoglobin probably after its rapid reduction to the cationic trivalent state within the RBC (Gray and Sterling, 1950). The aims of this study were to investigate the effect of the presence of chromium in RBCs on oxygen uptake capacity of blood [oxyhaemoglobin saturation (O2-SAT) at specific pO2 values] and to evaluate the suitability of the two commonly used anti-coagulants, heparin and EDTA, for oxygen uptake studies.
LanguageEnglish
Pages15-16
Number of pages1
JournalToxicology
Volume253
Issue number1-3
DOIs
Publication statusPublished - 2008

Fingerprint

Chromium
Hemoglobins
Blood
Erythrocytes
Cells
Oxygen
Anions
Chromium Alloys
Chromates
Coagulants
Oxyhemoglobins
Metals
Stainless Steel
Orthopedics
Cell membranes
Edetic Acid
Corrosion
Heparin
Hip
Mothers

Keywords

  • chromium
  • red blood cells
  • haemoglobin
  • oxygen
  • bioengineering

Cite this

Afolaranmi, G. A., Tettey, J. N. A., Gaylor, J. D. S., Murray, H. M., Meek, R. M. D., & Grant, M. H. (2008). Uptake of chromium into red blood cells does not affect the ability of haemoglobin to bind oxygen. Toxicology, 253(1-3), 15-16. https://doi.org/10.1016/j.tox.2008.07.017
Afolaranmi, G.A. ; Tettey, J.N.A. ; Gaylor, J.D.S. ; Murray, H.M. ; Meek, R.M.D. ; Grant, M.H. / Uptake of chromium into red blood cells does not affect the ability of haemoglobin to bind oxygen. In: Toxicology. 2008 ; Vol. 253, No. 1-3. pp. 15-16.
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Afolaranmi, GA, Tettey, JNA, Gaylor, JDS, Murray, HM, Meek, RMD & Grant, MH 2008, 'Uptake of chromium into red blood cells does not affect the ability of haemoglobin to bind oxygen' Toxicology, vol. 253, no. 1-3, pp. 15-16. https://doi.org/10.1016/j.tox.2008.07.017

Uptake of chromium into red blood cells does not affect the ability of haemoglobin to bind oxygen. / Afolaranmi, G.A.; Tettey, J.N.A.; Gaylor, J.D.S.; Murray, H.M.; Meek, R.M.D.; Grant, M.H.

In: Toxicology, Vol. 253, No. 1-3, 2008, p. 15-16.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Uptake of chromium into red blood cells does not affect the ability of haemoglobin to bind oxygen

AU - Afolaranmi, G.A.

AU - Tettey, J.N.A.

AU - Gaylor, J.D.S.

AU - Murray, H.M.

AU - Meek, R.M.D.

AU - Grant, M.H.

N1 - Proceedings of the Annual Congress of The British Toxicology Society

PY - 2008

Y1 - 2008

N2 - Many of the metallic implants used in orthopaedics are made of stainless steel or cobalt-chromium alloys which contain 18-30% chromium. Hexavalent chromium (Cr VI) has been shown to be the predominant form of chromium released following in vivo and in vitro corrosion of these metal implants (Merritt and Brown, 1995 K. Merritt and S.A. Brown, J. Biomed. Mater. Res. 29 (1995), pp. 627-633. Full Text via CrossRef | View Record in Scopus | Cited By in Scopus (67)Merritt and Brown, 1995). Blood chromium levels may be elevated between 50 and 250 times in patients with metal hip implants (Lhotka et al., 2003). At physiological pH, Cr (VI) exists predominantly as the chromate anion and as such can enter cells via non-specific anion channels. The anionic Cr (VI) diffuses readily through the red blood cell (RBC) membrane and is bound by the haemoglobin probably after its rapid reduction to the cationic trivalent state within the RBC (Gray and Sterling, 1950). The aims of this study were to investigate the effect of the presence of chromium in RBCs on oxygen uptake capacity of blood [oxyhaemoglobin saturation (O2-SAT) at specific pO2 values] and to evaluate the suitability of the two commonly used anti-coagulants, heparin and EDTA, for oxygen uptake studies.

AB - Many of the metallic implants used in orthopaedics are made of stainless steel or cobalt-chromium alloys which contain 18-30% chromium. Hexavalent chromium (Cr VI) has been shown to be the predominant form of chromium released following in vivo and in vitro corrosion of these metal implants (Merritt and Brown, 1995 K. Merritt and S.A. Brown, J. Biomed. Mater. Res. 29 (1995), pp. 627-633. Full Text via CrossRef | View Record in Scopus | Cited By in Scopus (67)Merritt and Brown, 1995). Blood chromium levels may be elevated between 50 and 250 times in patients with metal hip implants (Lhotka et al., 2003). At physiological pH, Cr (VI) exists predominantly as the chromate anion and as such can enter cells via non-specific anion channels. The anionic Cr (VI) diffuses readily through the red blood cell (RBC) membrane and is bound by the haemoglobin probably after its rapid reduction to the cationic trivalent state within the RBC (Gray and Sterling, 1950). The aims of this study were to investigate the effect of the presence of chromium in RBCs on oxygen uptake capacity of blood [oxyhaemoglobin saturation (O2-SAT) at specific pO2 values] and to evaluate the suitability of the two commonly used anti-coagulants, heparin and EDTA, for oxygen uptake studies.

KW - chromium

KW - red blood cells

KW - haemoglobin

KW - oxygen

KW - bioengineering

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DO - 10.1016/j.tox.2008.07.017

M3 - Article

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