Untargeted metabolomics of extracts from faecal samples demonstrates distinct differences between paediatric Crohn's disease patients and healthy controls but no significant changes resulting from exclusive enteral nutrition treatment

Adel Alghamdi, Konstantinos Gerasimidis, Gavin Blackburn, Didem Akinci, Christine Edwards, Richard K. Russell, David G. Watson

Research output: Contribution to journalArticle

Abstract

Metabolomic profiling using high resolution mass spectrometry with hydrophilic interaction chromatography was applied to 11 faecal extracts from eleven healthy children and to 43 faecal extracts from eleven children undergoing exclusive enteral nutrition for the treatment of active Crohn's disease (CD) at timepoints before, during (15, 30, and 60 days), and after treatment. Differences between the control and CD samples were identified at each timepoint. An orthogonal partial least square-discriminant analysis (OPLS-DA) model identified eight metabolites that were normally distributed according to Q-Q plots. The OPLS-DA model was able to discriminate the CD samples from the controls at every timepoint, but the model was not able to differentiate the CD samples from one another at the different timepoints during treatment with exclusive enteral nutrition. The differentiated metabolites identified in the CD samples included tyrosine, an ornithine isomer, arachidonic acid, eicosatrienoic acid, docosatetraenoic acid, a sphingomyelin, a ceramide, and dimethylsphinganine. Despite successful treatment, underlying differences remained in the metabolome of the CD patients. These differences dominated the separation of the samples when multivariate methods were applied.

LanguageEnglish
Article number82
Number of pages18
JournalMetabolites
Volume8
Issue number4
DOIs
Publication statusPublished - 22 Nov 2018

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Pediatrics
Metabolomics
Enteral Nutrition
Nutrition
Crohn Disease
Discriminant Analysis
Discriminant analysis
Metabolites
Least-Squares Analysis
Therapeutics
Acids
Ornithine
Metabolome
Sphingomyelins
Ceramides
Chromatography
Hydrophobic and Hydrophilic Interactions
Arachidonic Acid
Isomers
Mass spectrometry

Keywords

  • Crohn's disease
  • EEN
  • metabolomics
  • LC-MS
  • multivariate analysis

Cite this

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title = "Untargeted metabolomics of extracts from faecal samples demonstrates distinct differences between paediatric Crohn's disease patients and healthy controls but no significant changes resulting from exclusive enteral nutrition treatment",
abstract = "Metabolomic profiling using high resolution mass spectrometry with hydrophilic interaction chromatography was applied to 11 faecal extracts from eleven healthy children and to 43 faecal extracts from eleven children undergoing exclusive enteral nutrition for the treatment of active Crohn's disease (CD) at timepoints before, during (15, 30, and 60 days), and after treatment. Differences between the control and CD samples were identified at each timepoint. An orthogonal partial least square-discriminant analysis (OPLS-DA) model identified eight metabolites that were normally distributed according to Q-Q plots. The OPLS-DA model was able to discriminate the CD samples from the controls at every timepoint, but the model was not able to differentiate the CD samples from one another at the different timepoints during treatment with exclusive enteral nutrition. The differentiated metabolites identified in the CD samples included tyrosine, an ornithine isomer, arachidonic acid, eicosatrienoic acid, docosatetraenoic acid, a sphingomyelin, a ceramide, and dimethylsphinganine. Despite successful treatment, underlying differences remained in the metabolome of the CD patients. These differences dominated the separation of the samples when multivariate methods were applied.",
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Untargeted metabolomics of extracts from faecal samples demonstrates distinct differences between paediatric Crohn's disease patients and healthy controls but no significant changes resulting from exclusive enteral nutrition treatment. / Alghamdi, Adel; Gerasimidis, Konstantinos; Blackburn, Gavin; Akinci, Didem; Edwards, Christine; Russell, Richard K.; Watson, David G.

In: Metabolites, Vol. 8, No. 4, 82, 22.11.2018.

Research output: Contribution to journalArticle

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AU - Gerasimidis, Konstantinos

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AU - Edwards, Christine

AU - Russell, Richard K.

AU - Watson, David G.

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