Projects per year
Abstract
Surface-enhanced Raman scattering (SERS) is a promising imaging modality for use in a variety of multiplexed tracking and sensing applications in biological environments. However, the uniform production of SERS nanoparticle tags with high yield and brightness still remains a significant challenge. Here, we describe an approach based on the controlled co-adsorption of multiple dye species onto gold nanorods to create tags that can be detected across a much wider range of excitation wavelengths (514 – 1064 nm) compared to conventional approaches that typically focus on a single wavelength. This was achieved without the added complexity of nanoparticle aggregation or growing surrounding metallic shells to further enhance the surface-enhanced resonance Raman scattering (SERRS) signal. Correlated Raman and scanning electron microscopy mapping measurements of individual tags were used to clearly demonstrate that strong and reproducible SERRS signals at high particle yields (>92 %) were readily achievable. The polyelectrolyte-wrapped nanorod-dye conjugates were also found to be highly stable as well as non-cytotoxic. To demonstrate the use of these universal tags for the multimodal optical imaging of biological specimens, confocal Raman and fluorescence maps of stained immune cells following nanoparticle uptake were acquired at several excitation wavelengths and compared with dark-field images. The ability to colocalize and track individual
optically encoded nanoparticles across a wide range of wavelengths simultaneously will enable the use of SERS alongside other imaging techniques for the real-time monitoring of cell-nanoparticle interactions.
optically encoded nanoparticles across a wide range of wavelengths simultaneously will enable the use of SERS alongside other imaging techniques for the real-time monitoring of cell-nanoparticle interactions.
Original language | English |
---|---|
Pages (from-to) | 8600-8609 |
Number of pages | 10 |
Journal | ACS Nano |
Volume | 8 |
Issue number | 8 |
Early online date | 8 Aug 2014 |
DOIs | |
Publication status | Published - 26 Aug 2014 |
Keywords
- surface-enhanced Raman scattering
- nanoparticle aggregation
- multimodal optical imaging
- SERS
- SERS nanoparticle tags
Fingerprint
Dive into the research topics of 'Universal surface-enhanced Raman tags: individual nanorods for measurements from the visible to the infrared (514 – 1064 nm)'. Together they form a unique fingerprint.Profiles
Projects
- 2 Finished
-
Bioaffinity detection and tracking of disease biomarkers via dynamic multi modal surface plasmon enhanced nanoscopy
Wark, A. (Principal Investigator)
EPSRC (Engineering and Physical Sciences Research Council)
1/02/10 → 30/04/12
Project: Research
-
Medical Devices Doctoral Training Centre Renewal
Connolly, P. (Principal Investigator)
EPSRC (Engineering and Physical Sciences Research Council)
1/10/08 → 31/03/18
Project: Research - Studentship