Understanding protease catalysed solid phase peptide synthesis

R.V. Ulijn, N. Bisek, P.J. Halling, S.L. Flitsch

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

A protease (thermolysin) was used to directly synthesise a number of dipeptides from soluble Fmoc-amino acids onto a solid support (PEGA1900) in bulk aqueous media, often in very good yields. This shift in equilibrium toward synthesis is remarkable because for soluble dipeptides in aqueous solution hydrolysis rather than synthesis is observed. Three possible reasons for the equilibrium shift were considered: (i) using a solid support makes it easy to use an excess of reagents, so mass action contributes towards synthesis; (ii) reduction in the unfavourable hydrophobic hydration of the Fmoc group within the solid support compared with the free amino acid in solution and (iii) suppression of the ionization of amino groups linked to the solid phase due to mutual electrostatic repulsion. It was found that under the conditions studied the second effect was most important.
Original languageEnglish
Pages (from-to)1277-1281
Number of pages4
JournalOrganic and Biomolecular Chemistry
Volume1
Issue number8
DOIs
Publication statusPublished - 2003

Keywords

  • protease
  • dipeptides
  • Fmoc-amino acids
  • reagents
  • hydrophobic hydration
  • mutual electrostatic repulsion

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