Understanding enzyme action on immobilised substrates

P.J. Halling, R.V. Ulijn, S.L. Flitsch

Research output: Contribution to journalArticle

49 Citations (Scopus)

Abstract

With increasing interest in automated synthesis and screening protocols, solid supported chemistry and biochemistry are attractive technologies. Studies with surface-immobilised substrates have been carried out to analyse enzyme accessibility, kinetics and thermodynamics. Several interesting new methods have been developed to monitor enzyme action on substrates attached to a solid phase such as polymer beads glass or gold surfaces. These include fluorescence measurements, MALDI-TOF mass spectrometry, and the use of quartz crystal microbalances to measure weight changes of immobilised molecules directly on the surface. Approaches that allow spatial resolution in single beads have also been reported. The ability of enzymes to reach the inside of beads is becoming better characterised and new supports have been developed that allow improved accessibility. The equilibrium position of reactions on the solid surface can be substantially shifted compared with reactions in solution, and this can be usefully exploited using hydrolases in reverse. Research is also starting to tackle the way in which kinetics are modified when the substrates are surface immobilised.
LanguageEnglish
Pages385-392
Number of pages7
JournalCurrent Opinion in Biotechnology
Volume16
Issue number4
DOIs
Publication statusPublished - 2005

Fingerprint

Enzymes
Substrates
Quartz Crystal Microbalance Techniques
Matrix-Assisted Laser Desorption-Ionization Mass Spectrometry
Hydrolases
Thermodynamics
Gold
Biochemistry
Glass
Mass Spectrometry
Polymers
Fluorescence
Enzyme kinetics
Quartz crystal microbalances
Technology
Weights and Measures
Mass spectrometry
Screening
Research
Molecules

Keywords

  • screening protocols
  • kinetics
  • thermodynamics
  • MALDI-TOF mass spectrometry
  • substrates

Cite this

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Understanding enzyme action on immobilised substrates. / Halling, P.J.; Ulijn, R.V.; Flitsch, S.L.

In: Current Opinion in Biotechnology, Vol. 16, No. 4, 2005, p. 385-392.

Research output: Contribution to journalArticle

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