Ultrasensitive and ultrawide range detection of a cardiac biomarker on a surface plasmon resonance platform

Hye Ri Jang, Alastair Wark, Seung Hee Baek, Bong Hyun Chung, Hye Jin Lee

Research output: Contribution to journalArticlepeer-review

55 Citations (Scopus)

Abstract

One of the main challenges in the development of new analytical platforms for ultrasensitive bioaffinity detection is jointly achieving a wide dynamic range in target analyte concentration, especially for approaches that rely on multistep processes as a part of the signal amplification mechanism. In this paper, a new surface-based sandwich assay is introduced for the direct detection of B-type natriuretic peptide (BNP), an important biomarker for cardiac failure, at concentrations ranging from 1 aM to 500 nM. This was achieved using nanoparticle-enhanced surface plasmon resonance (SPR) where a DNA aptamer is immobilized on a chemically modified gold surface in conjunction with the specific adsorption of antiBNP coated gold nanocubes in the presence of the biomarker target. A concentration detection range greater than eleven orders of magnitude was achieved through dynamic control of only the secondary nanoparticle probe concentration. Furthermore, detection at low attomolar concentrations was also achieved in undiluted human serum.
Original languageEnglish
Pages (from-to)814-819
Number of pages6
JournalAnalytical Chemistry
Volume86
Issue number1
Early online date13 Dec 2013
DOIs
Publication statusPublished - 2014

Keywords

  • signal amplification
  • surface plasmon resonance (SPR)
  • chemically modified gold surface
  • coated gold nanocubes

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