Ultrasensitive and ultrawide range detection of a cardiac biomarker on a surface plasmon resonance platform

Hye Ri Jang, Alastair Wark, Seung Hee Baek, Bong Hyun Chung, Hye Jin Lee

Research output: Contribution to journalArticle

43 Citations (Scopus)

Abstract

One of the main challenges in the development of new analytical platforms for ultrasensitive bioaffinity detection is jointly achieving a wide dynamic range in target analyte concentration, especially for approaches that rely on multistep processes as a part of the signal amplification mechanism. In this paper, a new surface-based sandwich assay is introduced for the direct detection of B-type natriuretic peptide (BNP), an important biomarker for cardiac failure, at concentrations ranging from 1 aM to 500 nM. This was achieved using nanoparticle-enhanced surface plasmon resonance (SPR) where a DNA aptamer is immobilized on a chemically modified gold surface in conjunction with the specific adsorption of antiBNP coated gold nanocubes in the presence of the biomarker target. A concentration detection range greater than eleven orders of magnitude was achieved through dynamic control of only the secondary nanoparticle probe concentration. Furthermore, detection at low attomolar concentrations was also achieved in undiluted human serum.
LanguageEnglish
Pages814-819
Number of pages6
JournalAnalytical Chemistry
Volume86
Issue number1
Early online date13 Dec 2013
DOIs
Publication statusPublished - 2014

Fingerprint

Surface plasmon resonance
Biomarkers
Gold
Nucleotide Aptamers
Nanoparticles
Brain Natriuretic Peptide
Amplification
Assays
Adsorption

Keywords

  • signal amplification
  • surface plasmon resonance (SPR)
  • chemically modified gold surface
  • coated gold nanocubes

Cite this

Jang, Hye Ri ; Wark, Alastair ; Baek, Seung Hee ; Chung, Bong Hyun ; Lee, Hye Jin. / Ultrasensitive and ultrawide range detection of a cardiac biomarker on a surface plasmon resonance platform. In: Analytical Chemistry. 2014 ; Vol. 86, No. 1. pp. 814-819.
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Ultrasensitive and ultrawide range detection of a cardiac biomarker on a surface plasmon resonance platform. / Jang, Hye Ri; Wark, Alastair; Baek, Seung Hee; Chung, Bong Hyun ; Lee, Hye Jin.

In: Analytical Chemistry, Vol. 86, No. 1, 2014, p. 814-819.

Research output: Contribution to journalArticle

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T1 - Ultrasensitive and ultrawide range detection of a cardiac biomarker on a surface plasmon resonance platform

AU - Jang, Hye Ri

AU - Wark, Alastair

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AU - Chung, Bong Hyun

AU - Lee, Hye Jin

PY - 2014

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AB - One of the main challenges in the development of new analytical platforms for ultrasensitive bioaffinity detection is jointly achieving a wide dynamic range in target analyte concentration, especially for approaches that rely on multistep processes as a part of the signal amplification mechanism. In this paper, a new surface-based sandwich assay is introduced for the direct detection of B-type natriuretic peptide (BNP), an important biomarker for cardiac failure, at concentrations ranging from 1 aM to 500 nM. This was achieved using nanoparticle-enhanced surface plasmon resonance (SPR) where a DNA aptamer is immobilized on a chemically modified gold surface in conjunction with the specific adsorption of antiBNP coated gold nanocubes in the presence of the biomarker target. A concentration detection range greater than eleven orders of magnitude was achieved through dynamic control of only the secondary nanoparticle probe concentration. Furthermore, detection at low attomolar concentrations was also achieved in undiluted human serum.

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