Ultrabroadband terahertz spectroscopies of biomolecules and water

David Turton, Thomas Harwood, Adrian Lapthorn, Elizabeth Ellis, Klaas Wynne

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

We describe the use of a range of modern spectroscopic techniques—from terahertz time-domain spectroscopy (THz- TDS) to high dynamic-range femtosecond optical Kerr-effect (OKE) spectroscopy—to study the interaction of proteins, peptides, and other biomolecules with the aqueous solvent. Chemical reactivity in proteins requires fast picosecond fluctuations to reach the transition state, to dissipate energy, and (possibly) to reduce the width and height of energy barriers along the reaction coordinate. Such motions are linked with the structure and dynamics of the aqueous solvent making hydration critical to function. These dynamics take place over a huge range of timescales: from the nanosecond timescale of diffusion of water molecules in the first solvation shell of proteins, picosecond motions of amino-acid side chains, and sub-picosecond librational and phonon-like motions of water. It is shown that a large range of frequencies from MHz to THz is accessible directly using OKE resulting in the reduced anisotropic Raman spectrum and by using a combination of techniques including THz-TDS resulting in the dielectric spectrum. Using these techniques, we can now observe very significant differences in the spectra of proteins in aqueous solvent in the 3-30 THz range and more subtle differences at lower frequencies (10 GHz-3 THz)
LanguageEnglish
Article number862303
JournalProceedings of SPIE: The International Society for Optical Engineering
Volume8623
DOIs
Publication statusPublished - 14 Mar 2013

Fingerprint

Terahertz Spectroscopy
Terahertz spectroscopy
Biomolecules
Optical Kerr effect
proteins
Proteins
Protein
Water
Kerr effects
Range of data
water
spectroscopy
Motion
Spectroscopy
THz-TDS
Time Scales
Dissipate
Chemical reactivity
Transition State
High Dynamic Range

Keywords

  • chemicals
  • diffusion
  • Kerr effect
  • molecules
  • phonons
  • spectroscopy
  • terahertz radiation
  • water

Cite this

Turton, David ; Harwood, Thomas ; Lapthorn, Adrian ; Ellis, Elizabeth ; Wynne, Klaas. / Ultrabroadband terahertz spectroscopies of biomolecules and water. In: Proceedings of SPIE: The International Society for Optical Engineering. 2013 ; Vol. 8623.
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Ultrabroadband terahertz spectroscopies of biomolecules and water. / Turton, David; Harwood, Thomas; Lapthorn, Adrian; Ellis, Elizabeth; Wynne, Klaas.

In: Proceedings of SPIE: The International Society for Optical Engineering, Vol. 8623, 862303, 14.03.2013.

Research output: Contribution to journalArticle

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