Tyrosine rotamer states in beta amyloid: signatures of aggregation and fibrillation

Onorio Mancini, Olaf J. Rolinski, Karina Kubiak-Ossowska, Paul A. Mulheran

Research output: Contribution to journalArticle

Abstract

During the early stages of β amyloid (Ab) peptide aggregation, toxic oligomers form which have been recognized as a likely cause of Alzheimer's disease. In this work, we use fully atomistic molecular dynamics simulation to study the amorphous aggregation of the peptide as well as model β-sheet protofibril structures. In particular, we study the rotamer states of the single fluorescent tyrosine (Tyr) residue present in each Ab. We find that the occupation of the four previously identified rotamers is different for monomeric and amorphous aggregates because of the differing environments of the Tyr side-chains. Surprisingly, we also identify two new rotamers that uniquely appear for the β-sheet structures, so that together the rotamers provide distinct signatures for the different stages of aggregation and fibrillation. We propose that these rotamers could be identified in fluorescence spectroscopy, with each rotamer having a distinct fluorescence lifetime because of its different exposures to the solvent. The identification of the two new rotamers therefore provides a new means to probe amyloid formation kinetics and to monitor the effect of additives including prospective drugs.

LanguageEnglish
Pages16046-16056
Number of pages11
JournalACS Omega
Volume3
Issue number11
DOIs
Publication statusPublished - 27 Nov 2018

Fingerprint

Amyloid
Tyrosine
Agglomeration
Peptides
Poisons
Fluorescence spectroscopy
Oligomers
Molecular dynamics
Fluorescence
Kinetics
Computer simulation
Pharmaceutical Preparations

Keywords

  • amyloid
  • amyloid-β peptide
  • amyloid beta-peptides
  • aggregation
  • fibrillation
  • tyrosine rotamer states
  • toxic oligomers
  • alzheimer's disease

Cite this

Mancini, Onorio ; Rolinski, Olaf J. ; Kubiak-Ossowska, Karina ; Mulheran, Paul A. / Tyrosine rotamer states in beta amyloid : signatures of aggregation and fibrillation. In: ACS Omega. 2018 ; Vol. 3, No. 11. pp. 16046-16056.
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Tyrosine rotamer states in beta amyloid : signatures of aggregation and fibrillation. / Mancini, Onorio; Rolinski, Olaf J.; Kubiak-Ossowska, Karina; Mulheran, Paul A.

In: ACS Omega, Vol. 3, No. 11, 27.11.2018, p. 16046-16056.

Research output: Contribution to journalArticle

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