We report a robust and reliable platform source for visible-wavelength multiphoton microscopy that is based on nonlinear optical methods. We demonstrate a synchronously pumped, singly resonant optical parametric oscillator with simultaneous intracavity third-order quasi-phase matching in a single crystal that generates continuously tunable, visible, and femtosecond-pulsed radiation. The application of the system is demonstrated by two-photon laser-scanning fluorescence microscopy of rabbit cardiac myocytes loaded with the fluorescent Ca 2+ indicator fura-2.
|Number of pages||2|
|Publication status||Published - 2003|
McConnell, G., Smith, G. L., Girkin, J. M., Gurney, A. M., & Ferguson, A. I. (2003). Two-photon microscopy of fura-2-loaded cardiac myocytes with an all-solid-state tunable and visible femtosecond laser source. Optics Letters, 28(19), 1742-1744.