Two-photon microscopy of fura-2-loaded cardiac myocytes with an all-solid-state tunable and visible femtosecond laser source

G. McConnell, G.L. Smith, J.M. Girkin, A.M. Gurney, A.I. Ferguson

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

We report a robust and reliable platform source for visible-wavelength multiphoton microscopy that is based on nonlinear optical methods. We demonstrate a synchronously pumped, singly resonant optical parametric oscillator with simultaneous intracavity third-order quasi-phase matching in a single crystal that generates continuously tunable, visible, and femtosecond-pulsed radiation. The application of the system is demonstrated by two-photon laser-scanning fluorescence microscopy of rabbit cardiac myocytes loaded with the fluorescent Ca 2+ indicator fura-2.
Original languageEnglish
Pages (from-to)1742-1744
Number of pages2
JournalOptics Letters
Volume28
Issue number19
Publication statusPublished - 2003

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