Two-photon microscopy of fura-2-loaded cardiac myocytes with an all-solid-state tunable and visible femtosecond laser source

G. McConnell; G.L. Smith; J.M. Girkin; A.M. Gurney; A.I. Ferguson

Two-photon microscopy of fura-2-loaded cardiac myocytes with an all-solid-state tunable and visible femtosecond laser source

G. McConnell, G.L. Smith, J.M. Girkin, A.M. Gurney, A.I. Ferguson

Research output: Contribution to journal › Article › peer-review

Abstract

We report a robust and reliable platform source for visible-wavelength multiphoton microscopy that is based on nonlinear optical methods. We demonstrate a synchronously pumped, singly resonant optical parametric oscillator with simultaneous intracavity third-order quasi-phase matching in a single crystal that generates continuously tunable, visible, and femtosecond-pulsed radiation. The application of the system is demonstrated by two-photon laser-scanning fluorescence microscopy of rabbit cardiac myocytes loaded with the fluorescent Ca 2+ indicator fura-2.

Original language	English
Pages (from-to)	1742-1744
Number of pages	2
Journal	Optics Letters
Volume	28
Issue number	19
Publication status	Published - 2003

Cite this

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title = "Two-photon microscopy of fura-2-loaded cardiac myocytes with an all-solid-state tunable and visible femtosecond laser source",

abstract = "We report a robust and reliable platform source for visible-wavelength multiphoton microscopy that is based on nonlinear optical methods. We demonstrate a synchronously pumped, singly resonant optical parametric oscillator with simultaneous intracavity third-order quasi-phase matching in a single crystal that generates continuously tunable, visible, and femtosecond-pulsed radiation. The application of the system is demonstrated by two-photon laser-scanning fluorescence microscopy of rabbit cardiac myocytes loaded with the fluorescent Ca 2+ indicator fura-2.",

author = "G. McConnell and G.L. Smith and J.M. Girkin and A.M. Gurney and A.I. Ferguson",

year = "2003",

language = "English",

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pages = "1742--1744",

journal = "Optics Letters",

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publisher = "Optical Society of America",

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T1 - Two-photon microscopy of fura-2-loaded cardiac myocytes with an all-solid-state tunable and visible femtosecond laser source

AU - McConnell, G.

AU - Smith, G.L.

AU - Girkin, J.M.

AU - Gurney, A.M.

AU - Ferguson, A.I.

PY - 2003

Y1 - 2003

N2 - We report a robust and reliable platform source for visible-wavelength multiphoton microscopy that is based on nonlinear optical methods. We demonstrate a synchronously pumped, singly resonant optical parametric oscillator with simultaneous intracavity third-order quasi-phase matching in a single crystal that generates continuously tunable, visible, and femtosecond-pulsed radiation. The application of the system is demonstrated by two-photon laser-scanning fluorescence microscopy of rabbit cardiac myocytes loaded with the fluorescent Ca 2+ indicator fura-2.

AB - We report a robust and reliable platform source for visible-wavelength multiphoton microscopy that is based on nonlinear optical methods. We demonstrate a synchronously pumped, singly resonant optical parametric oscillator with simultaneous intracavity third-order quasi-phase matching in a single crystal that generates continuously tunable, visible, and femtosecond-pulsed radiation. The application of the system is demonstrated by two-photon laser-scanning fluorescence microscopy of rabbit cardiac myocytes loaded with the fluorescent Ca 2+ indicator fura-2.

UR - http://dx.doi.org/doi:10.1364/OL.28.001742

M3 - Article

SN - 0146-9592

VL - 28

SP - 1742

EP - 1744

JO - Optics Letters

JF - Optics Letters

IS - 19

ER -

Two-photon microscopy of fura-2-loaded cardiac myocytes with an all-solid-state tunable and visible femtosecond laser source

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