Two-photon laser scanning fluorescence microscopy using photonic crystal fibre

Research output: Contribution to journalArticle

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Abstract

We report the application of a simple yet powerful modular pulse compression system, based on photonic crystal fibres which improves upon incumbent twophoton laser scanning fluorescence microscopy techniques. This system provided more than a 7-fold increase in fluorescence yield when compared with a commercial two-photon microscopy system. From this, we infer pulses of infrared radiaton of less than 35 fs duration reaching the sample.
Original languageEnglish
Pages (from-to)922-927
Number of pages5
JournalJournal of Biomedical Optics
Volume9
Issue number5
DOIs
Publication statusPublished - 2004

Fingerprint

Pulse compression
Photonic crystal fibers
Fluorescence microscopy
Microscopic examination
Photons
Fluorescence
photonics
microscopy
Infrared radiation
Scanning
fluorescence
scanning
fibers
Lasers
pulse compression
photons
crystals
lasers
pulses

Keywords

  • lasers
  • optics
  • photonics
  • microscopy

Cite this

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Two-photon laser scanning fluorescence microscopy using photonic crystal fibre. / McConnell, G.; Riis, E.

In: Journal of Biomedical Optics, Vol. 9, No. 5, 2004, p. 922-927.

Research output: Contribution to journalArticle

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AB - We report the application of a simple yet powerful modular pulse compression system, based on photonic crystal fibres which improves upon incumbent twophoton laser scanning fluorescence microscopy techniques. This system provided more than a 7-fold increase in fluorescence yield when compared with a commercial two-photon microscopy system. From this, we infer pulses of infrared radiaton of less than 35 fs duration reaching the sample.

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KW - optics

KW - photonics

KW - microscopy

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