Two-photon laser scanning fluorescence microscopy using photonic crystal fibre

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Abstract

We report the application of a simple yet powerful modular pulse compression system, based on photonic crystal fibres which improves upon incumbent twophoton laser scanning fluorescence microscopy techniques. This system provided more than a 7-fold increase in fluorescence yield when compared with a commercial two-photon microscopy system. From this, we infer pulses of infrared radiaton of less than 35 fs duration reaching the sample.
Original languageEnglish
Pages (from-to)922-927
Number of pages5
JournalJournal of Biomedical Optics
Volume9
Issue number5
DOIs
Publication statusPublished - 2004

Keywords

  • lasers
  • optics
  • photonics
  • microscopy

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