Two-photon excited surface plasmon enhanced energy transfer between DAPI and gold nanoparticles: opportunities in intra-cellular imaging and sensing

Yinan Zhang; David J. S. Birch; Yu Chen

doi:10.1063/1.3633066

Two-photon excited surface plasmon enhanced energy transfer between DAPI and gold nanoparticles: opportunities in intra-cellular imaging and sensing

Yinan Zhang, David J. S. Birch, Yu Chen

Research output: Contribution to journal › Article › peer-review

36 Citations (Scopus)

Abstract

We have demonstrated energy transfer between 4'-6-Diamidino-2-phenylindole (DAPI), a commonly used DNA label, and gold nanoparticles under two-photon excitation in solution using fluorescence lifetime imaging microscopy (FLIM). With comparable size and concentration, gold nanorods (GNRs) are shown to provide more efficient energy transfer than gold nanospheres (GNSs). We attribute this transfer enhancement effect to the longitudinal surface plasmon mode of GNRs overlapping with the excitation wavelength. Energy transfer under two-photon excitation between GNRs and DAPI has also been observed in cell culture and found to be in accord with the solution phase results.

Original language	English
Article number	103701
Pages (from-to)	-
Number of pages	3
Journal	Applied Physics Letters
Volume	99
Issue number	10
DOIs	https://doi.org/10.1063/1.3633066
Publication status	Published - 5 Sept 2011

Keywords

biomedical optical imaging
cellular biophysics
excited states
fluorescence
gold
nanomedicine
nanoparticles
nanorods
nanosensors
surface plasmon resonance
two-photon processes
CELLS
resonance
TIC - Bionanotechnology

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10.1063/1.3633066

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title = "Two-photon excited surface plasmon enhanced energy transfer between DAPI and gold nanoparticles: opportunities in intra-cellular imaging and sensing",

abstract = "We have demonstrated energy transfer between 4'-6-Diamidino-2-phenylindole (DAPI), a commonly used DNA label, and gold nanoparticles under two-photon excitation in solution using fluorescence lifetime imaging microscopy (FLIM). With comparable size and concentration, gold nanorods (GNRs) are shown to provide more efficient energy transfer than gold nanospheres (GNSs). We attribute this transfer enhancement effect to the longitudinal surface plasmon mode of GNRs overlapping with the excitation wavelength. Energy transfer under two-photon excitation between GNRs and DAPI has also been observed in cell culture and found to be in accord with the solution phase results.",

keywords = "biomedical optical imaging, cellular biophysics, excited states, fluorescence, gold, nanomedicine, nanoparticles, nanorods, nanosensors, surface plasmon resonance, two-photon processes, CELLS, resonance, TIC - Bionanotechnology",

author = "Yinan Zhang and Birch, {David J. S.} and Yu Chen",

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T1 - Two-photon excited surface plasmon enhanced energy transfer between DAPI and gold nanoparticles: opportunities in intra-cellular imaging and sensing

AU - Zhang, Yinan

AU - Birch, David J. S.

AU - Chen, Yu

PY - 2011/9/5

Y1 - 2011/9/5

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AB - We have demonstrated energy transfer between 4'-6-Diamidino-2-phenylindole (DAPI), a commonly used DNA label, and gold nanoparticles under two-photon excitation in solution using fluorescence lifetime imaging microscopy (FLIM). With comparable size and concentration, gold nanorods (GNRs) are shown to provide more efficient energy transfer than gold nanospheres (GNSs). We attribute this transfer enhancement effect to the longitudinal surface plasmon mode of GNRs overlapping with the excitation wavelength. Energy transfer under two-photon excitation between GNRs and DAPI has also been observed in cell culture and found to be in accord with the solution phase results.

KW - biomedical optical imaging

KW - cellular biophysics

KW - excited states

KW - fluorescence

KW - gold

KW - nanomedicine

KW - nanoparticles

KW - nanorods

KW - nanosensors

KW - surface plasmon resonance

KW - two-photon processes

KW - CELLS

KW - resonance

KW - TIC - Bionanotechnology

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DO - 10.1063/1.3633066

M3 - Article

SN - 0003-6951

VL - 99

SP - -

JO - Applied Physics Letters

JF - Applied Physics Letters

IS - 10

M1 - 103701

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Two-photon excited surface plasmon enhanced energy transfer between DAPI and gold nanoparticles: opportunities in intra-cellular imaging and sensing

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NMS: EPSRC Science and Innovation Nanometrology for Molecular Science, Medicine and Manufacture

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Two-photon excited surface plasmon enhanced energy transfer between DAPI and gold nanoparticles: opportunities in intra-cellular imaging and sensing

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NMS: EPSRC Science and Innovation Nanometrology for Molecular Science, Medicine and Manufacture

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