Two-photon excited surface plasmon enhanced energy transfer between DAPI and gold nanoparticles: opportunities in intra-cellular imaging and sensing

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31 Citations (Scopus)

Abstract

We have demonstrated energy transfer between 4'-6-Diamidino-2-phenylindole (DAPI), a commonly used DNA label, and gold nanoparticles under two-photon excitation in solution using fluorescence lifetime imaging microscopy (FLIM). With comparable size and concentration, gold nanorods (GNRs) are shown to provide more efficient energy transfer than gold nanospheres (GNSs). We attribute this transfer enhancement effect to the longitudinal surface plasmon mode of GNRs overlapping with the excitation wavelength. Energy transfer under two-photon excitation between GNRs and DAPI has also been observed in cell culture and found to be in accord with the solution phase results.

LanguageEnglish
Article number103701
Pages-
Number of pages3
JournalApplied Physics Letters
Volume99
Issue number10
DOIs
Publication statusPublished - 5 Sep 2011

Fingerprint

energy transfer
gold
nanoparticles
nanorods
photons
excitation
deoxyribonucleic acid
microscopy
life (durability)
fluorescence
augmentation
wavelengths

Keywords

  • biomedical optical imaging
  • cellular biophysics
  • excited states
  • fluorescence
  • gold
  • nanomedicine
  • nanoparticles
  • nanorods
  • nanosensors
  • surface plasmon resonance
  • two-photon processes
  • CELLS
  • resonance
  • TIC - Bionanotechnology

Cite this

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abstract = "We have demonstrated energy transfer between 4'-6-Diamidino-2-phenylindole (DAPI), a commonly used DNA label, and gold nanoparticles under two-photon excitation in solution using fluorescence lifetime imaging microscopy (FLIM). With comparable size and concentration, gold nanorods (GNRs) are shown to provide more efficient energy transfer than gold nanospheres (GNSs). We attribute this transfer enhancement effect to the longitudinal surface plasmon mode of GNRs overlapping with the excitation wavelength. Energy transfer under two-photon excitation between GNRs and DAPI has also been observed in cell culture and found to be in accord with the solution phase results.",
keywords = "biomedical optical imaging, cellular biophysics, excited states, fluorescence, gold, nanomedicine, nanoparticles, nanorods, nanosensors, surface plasmon resonance, two-photon processes, CELLS, resonance, TIC - Bionanotechnology",
author = "Yinan Zhang and Birch, {David J. S.} and Yu Chen",
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AU - Zhang, Yinan

AU - Birch, David J. S.

AU - Chen, Yu

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AB - We have demonstrated energy transfer between 4'-6-Diamidino-2-phenylindole (DAPI), a commonly used DNA label, and gold nanoparticles under two-photon excitation in solution using fluorescence lifetime imaging microscopy (FLIM). With comparable size and concentration, gold nanorods (GNRs) are shown to provide more efficient energy transfer than gold nanospheres (GNSs). We attribute this transfer enhancement effect to the longitudinal surface plasmon mode of GNRs overlapping with the excitation wavelength. Energy transfer under two-photon excitation between GNRs and DAPI has also been observed in cell culture and found to be in accord with the solution phase results.

KW - biomedical optical imaging

KW - cellular biophysics

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KW - fluorescence

KW - gold

KW - nanomedicine

KW - nanoparticles

KW - nanorods

KW - nanosensors

KW - surface plasmon resonance

KW - two-photon processes

KW - CELLS

KW - resonance

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