Abstract
Nuclear magnetic resonance (NMR) is a powerful tool for investigating atomic-scale structure in heterogeneous or composite materials where long-range order is absent. In this work solid-state 1H and 1H-detected NMR experiments were performed with fast magic angle spinning (νR = 75 kHz) and at high magnetic fields (B0 = 20 T) and used to gain structural insight into a heterogeneous biocatalyst consisting of an enzyme, human carbonic anhydrase II (hCA II), covalently immobilized on epoxy-functionalized silica. Two-dimensional 1H-1H NOESY-type correlation experiments were able to provide information on 1H environments in silica, epoxy-silica and the immobilized enzyme. Two distinct signals originating from water protons were observed: water associated with the surface of the silica and the water associated with the immobilized enzyme. Additional two-dimensional 1H-1H double–single quantum (DQ-SQ) correlation experiments suggested that the immobilized enzyme is not in close contact with the silica surface. Most significantly, comparison of two-dimensional 1H-15N spectra of the immobilized enzyme and the solution-state enzyme confirmed that the structural integrity of the protein is well preserved upon covalent immobilization.
Original language | English |
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Pages (from-to) | 7-11 |
Number of pages | 5 |
Journal | Solid State Nuclear Magnetic Resonance |
Volume | 92 |
Early online date | 14 Mar 2018 |
DOIs | |
Publication status | E-pub ahead of print - 14 Mar 2018 |
Keywords
- solid-state NMR
- biocatalysis
- covalent immobilization
- epoxy-functionalized silica
- hCA II
- heterogeneous biocatalysts
- human carbonic anhydrase II
- immobilized enzymes