Projects per year
Homogeneous illumination in single-molecule localization microscopy (SMLM) is key for the quantitative analysis of super-resolution images. Therefore, different approaches for flat-field illumination have been introduced as alternative to the conventional Gaussian illumination. Here, we introduce a single microelectromechanical systems (MEMS) mirror as a tunable and cost-effective device for adapting wide-field illumination in SMLM. In flat-field mode the MEMS allowed for consistent SMLM metrics across the entire field of view. Employing single-molecule photoswitching, we developed a simple yet powerful routine to benchmark different illumination schemes on the basis of local emitter brightness and ON-state lifetime. Moreover, we propose that tuning the MEMS beyond optimal flat-field conditions enables to study the kinetics of photoswitchable fluorophores within a single acquisition.
|Number of pages||9|
|Early online date||25 Aug 2021|
|Publication status||Published - 15 Sept 2021|
- super-resolution microscopy
- tuneable flat-field illumination
- MEMS micromirror
- quantitative microscopy
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- 4 Finished
Tuneable photoswitches for chromatic aberration-free multicolour super-resolution imaging
Van de Linde, S.
1/03/18 → 31/05/21
Doctoral Training Partnership (DTP 2016-2017 University of Strathclyde) | Herdly, Lucas
Van de Linde, S., McConnell, G. & Herdly, L.
EPSRC (Engineering and Physical Sciences Research Council)
1/10/17 → 1/02/22
Project: Research Studentship - Internally Allocated
Data for: “Tuneable wide-field illumination and single-molecule photoswitching with a single MEMS mirror”
Herdly, L. (Creator), JANIN, P. (Contributor), Bauer, R. (Supervisor) & Van de Linde, S. (Supervisor), University of Strathclyde, 16 Aug 2021