Projects per year
Homogeneous illumination in single-molecule localization microscopy (SMLM) is key for the quantitative analysis of super-resolution images. Therefore, different approaches for flat-field illumination have been introduced as alternative to the conventional Gaussian illumination. Here, we introduce a single microelectromechanical systems (MEMS) mirror as a tunable and cost-effective device for adapting wide-field illumination in SMLM. In flat-field mode the MEMS allowed for consistent SMLM metrics across the entire field of view. Employing single-molecule photoswitching, we developed a simple yet powerful routine to benchmark different illumination schemes on the basis of local emitter brightness and ON-state lifetime. Moreover, we propose that tuning the MEMS beyond optimal flat-field conditions enables to study the kinetics of photoswitchable fluorophores within a single acquisition.
- super-resolution microscopy
- tuneable flat-field illumination
- MEMS micromirror
- quantitative microscopy
FingerprintDive into the research topics of 'Tunable wide-field illumination and single-molecule photoswitching with a single MEMS mirror'. Together they form a unique fingerprint.
1/10/17 → 1/12/21
Project: Research Studentship - Internally Allocated
1/03/18 → 31/05/21
Data for: “Tuneable wide-field illumination and single-molecule photoswitching with a single MEMS mirror”