Trypanosoma congolense: B-lymphocyte responses differ between trypanotolerant and trypanosusceptible cattle

K.A. Taylor, V. Lutje, D. Kennedy, E. Authie, A. Boulange, L. Logan-Henfrey, B. Gichuki, G. Gettinby

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Abstract

Trypanosomiasis is a serious constraint to livestock production in sub-Saharan Africa. Some breeds of cattle are genetically more resistant to the pathogenic effects of trypanosome infection. We measured B-cell activation and the quantity and isotype of antibody produced at the cellular level in six trypanotolerant N'Dama and five trypanosusceptible Boran cattle. The frequencies of spleen cells secreting total and parasite-specific IgM and IgG were measured prior to and 16, 28, and 35 days after a primary challenge withTrypanosoma congolense.Boran cattle had higher frequencies of splenic cells secreting IgM specific for trypanosome-derived variable surface glycoprotein (VSG), cysteine protease (congopain, CP), and heat shock protein (hsp70/BiP) and the nonparasite antigen, ovalbumin, than did N'Dama cattle. In contrast, the number of VSG-specific IgG-secreting cells was significantly greater in N'Dama than in Boran cattle. During infection, low titers of anti-VSG IgM were detected transiently in the serum of all animals. However, N'Dama had significantly more VSG-specific IgG in blood than Boran during infection. The peripheral blood mononuclear cell population of N'Dama cattle contained a higher percentage of surface IgM-positive B-cells prior to and throughout infection than were found in the blood of Boran. In addition, during infection N'Dama cattle had more circulating lymphocytes that could be activatedin vitroto undergo differentiation into IgM- and IgG-secreting cells. These findings demonstrate differences in the frequency of trypanosome-specific antibody-secreting cells in the spleen and in the activation state of B-cells in the blood between N'Dama and Boran cattle during a primary infection withT. congolense.
LanguageEnglish
Pages106-116
Number of pages10
JournalExperimental Parasitology
Volume83
Issue number1
DOIs
Publication statusPublished - Jun 1996

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Trypanosoma congolense
B-Lymphocytes
Trypanosomiasis
Membrane Glycoproteins
Immunoglobulin M
Immunoglobulin G
Infection
Spleen
Antibody-Producing Cells
Cysteine Proteases
Africa South of the Sahara
Ovalbumin
Livestock
Heat-Shock Proteins
Blood Cells
Parasites
Lymphocytes
Antigens

Keywords

  • monoclonal-antibodies
  • cysteine protease
  • boran cattle
  • identification
  • infection

Cite this

Taylor, K. A., Lutje, V., Kennedy, D., Authie, E., Boulange, A., Logan-Henfrey, L., ... Gettinby, G. (1996). Trypanosoma congolense: B-lymphocyte responses differ between trypanotolerant and trypanosusceptible cattle. Experimental Parasitology , 83(1), 106-116. https://doi.org/10.1006/expr.1996.0054
Taylor, K.A. ; Lutje, V. ; Kennedy, D. ; Authie, E. ; Boulange, A. ; Logan-Henfrey, L. ; Gichuki, B. ; Gettinby, G. / Trypanosoma congolense: B-lymphocyte responses differ between trypanotolerant and trypanosusceptible cattle. In: Experimental Parasitology . 1996 ; Vol. 83, No. 1. pp. 106-116.
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Taylor, KA, Lutje, V, Kennedy, D, Authie, E, Boulange, A, Logan-Henfrey, L, Gichuki, B & Gettinby, G 1996, 'Trypanosoma congolense: B-lymphocyte responses differ between trypanotolerant and trypanosusceptible cattle' Experimental Parasitology , vol. 83, no. 1, pp. 106-116. https://doi.org/10.1006/expr.1996.0054

Trypanosoma congolense: B-lymphocyte responses differ between trypanotolerant and trypanosusceptible cattle. / Taylor, K.A.; Lutje, V.; Kennedy, D.; Authie, E.; Boulange, A.; Logan-Henfrey, L.; Gichuki, B.; Gettinby, G.

In: Experimental Parasitology , Vol. 83, No. 1, 06.1996, p. 106-116.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Trypanosoma congolense: B-lymphocyte responses differ between trypanotolerant and trypanosusceptible cattle

AU - Taylor, K.A.

AU - Lutje, V.

AU - Kennedy, D.

AU - Authie, E.

AU - Boulange, A.

AU - Logan-Henfrey, L.

AU - Gichuki, B.

AU - Gettinby, G.

PY - 1996/6

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AB - Trypanosomiasis is a serious constraint to livestock production in sub-Saharan Africa. Some breeds of cattle are genetically more resistant to the pathogenic effects of trypanosome infection. We measured B-cell activation and the quantity and isotype of antibody produced at the cellular level in six trypanotolerant N'Dama and five trypanosusceptible Boran cattle. The frequencies of spleen cells secreting total and parasite-specific IgM and IgG were measured prior to and 16, 28, and 35 days after a primary challenge withTrypanosoma congolense.Boran cattle had higher frequencies of splenic cells secreting IgM specific for trypanosome-derived variable surface glycoprotein (VSG), cysteine protease (congopain, CP), and heat shock protein (hsp70/BiP) and the nonparasite antigen, ovalbumin, than did N'Dama cattle. In contrast, the number of VSG-specific IgG-secreting cells was significantly greater in N'Dama than in Boran cattle. During infection, low titers of anti-VSG IgM were detected transiently in the serum of all animals. However, N'Dama had significantly more VSG-specific IgG in blood than Boran during infection. The peripheral blood mononuclear cell population of N'Dama cattle contained a higher percentage of surface IgM-positive B-cells prior to and throughout infection than were found in the blood of Boran. In addition, during infection N'Dama cattle had more circulating lymphocytes that could be activatedin vitroto undergo differentiation into IgM- and IgG-secreting cells. These findings demonstrate differences in the frequency of trypanosome-specific antibody-secreting cells in the spleen and in the activation state of B-cells in the blood between N'Dama and Boran cattle during a primary infection withT. congolense.

KW - monoclonal-antibodies

KW - cysteine protease

KW - boran cattle

KW - identification

KW - infection

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