Trypanosoma congolense: A comparison of T-cell-mediated responses in lymph nodes of trypanotolerant and trypanosusceptible cattle during primary infection

V. Lutje, K.A. Taylor, D. Kennedy, E. Authie, A. Boulange, G. Gettinby

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

A comparison of T-cell-mediated immune responses in trypanotolerant N'Dama and susceptible Boran cattle during primary infection with tsetse-transmittedTrypanosoma congolensewas conducted to assess whether different patterns of T-cell activation occurred during trypanosome infection. Proliferation and IFN-γ synthesis in response to trypanosome antigens and to the mitogen Con A were measured in LNC before infection and 10 and 35 days postinfection. Phenotypic analysis of LNC was also carried out. No significant differences in thein vitroproliferation of LNC to VSG, to hsp70/BiP, or to Con A were detected between the breeds. In contrast, IFN-γ production in response to Con A was higher in Boran cattle at 35 days p.i. A reduction in the number of CD2+ and CD4+ T-cells and an increase in the percentage of B-cells, CD8+ T-cells, and γδ T-cells during infection in both N'Dama and Boran was revealed by cytofluorimetric analysis of lymph node cells.
LanguageEnglish
Pages320-329
Number of pages9
JournalExperimental Parasitology
Volume84
Issue number3
DOIs
Publication statusPublished - Dec 1996

Fingerprint

Trypanosoma congolense
Lymph Nodes
T-Lymphocytes
Infection
Trypanosomiasis
Mitogens
B-Lymphocytes
Antigens

Keywords

  • trypanosomiasis
  • bovine
  • Trypanosoma congolense
  • T-cell proliferative responses
  • IFN-gamma synthesis
  • surface phenotype

Cite this

Lutje, V. ; Taylor, K.A. ; Kennedy, D. ; Authie, E. ; Boulange, A. ; Gettinby, G. / Trypanosoma congolense: A comparison of T-cell-mediated responses in lymph nodes of trypanotolerant and trypanosusceptible cattle during primary infection. In: Experimental Parasitology . 1996 ; Vol. 84, No. 3. pp. 320-329.
@article{e415249006dc43cb8a9c7fe58b9f7017,
title = "Trypanosoma congolense: A comparison of T-cell-mediated responses in lymph nodes of trypanotolerant and trypanosusceptible cattle during primary infection",
abstract = "A comparison of T-cell-mediated immune responses in trypanotolerant N'Dama and susceptible Boran cattle during primary infection with tsetse-transmittedTrypanosoma congolensewas conducted to assess whether different patterns of T-cell activation occurred during trypanosome infection. Proliferation and IFN-γ synthesis in response to trypanosome antigens and to the mitogen Con A were measured in LNC before infection and 10 and 35 days postinfection. Phenotypic analysis of LNC was also carried out. No significant differences in thein vitroproliferation of LNC to VSG, to hsp70/BiP, or to Con A were detected between the breeds. In contrast, IFN-γ production in response to Con A was higher in Boran cattle at 35 days p.i. A reduction in the number of CD2+ and CD4+ T-cells and an increase in the percentage of B-cells, CD8+ T-cells, and γδ T-cells during infection in both N'Dama and Boran was revealed by cytofluorimetric analysis of lymph node cells.",
keywords = "trypanosomiasis, bovine, Trypanosoma congolense, T-cell proliferative responses, IFN-gamma synthesis, surface phenotype",
author = "V. Lutje and K.A. Taylor and D. Kennedy and E. Authie and A. Boulange and G. Gettinby",
year = "1996",
month = "12",
doi = "10.1006/expr.1996.0120",
language = "English",
volume = "84",
pages = "320--329",
journal = "Experimental Parasitology",
issn = "0014-4894",
number = "3",

}

Trypanosoma congolense: A comparison of T-cell-mediated responses in lymph nodes of trypanotolerant and trypanosusceptible cattle during primary infection. / Lutje, V.; Taylor, K.A.; Kennedy, D.; Authie, E.; Boulange, A.; Gettinby, G.

In: Experimental Parasitology , Vol. 84, No. 3, 12.1996, p. 320-329.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Trypanosoma congolense: A comparison of T-cell-mediated responses in lymph nodes of trypanotolerant and trypanosusceptible cattle during primary infection

AU - Lutje, V.

AU - Taylor, K.A.

AU - Kennedy, D.

AU - Authie, E.

AU - Boulange, A.

AU - Gettinby, G.

PY - 1996/12

Y1 - 1996/12

N2 - A comparison of T-cell-mediated immune responses in trypanotolerant N'Dama and susceptible Boran cattle during primary infection with tsetse-transmittedTrypanosoma congolensewas conducted to assess whether different patterns of T-cell activation occurred during trypanosome infection. Proliferation and IFN-γ synthesis in response to trypanosome antigens and to the mitogen Con A were measured in LNC before infection and 10 and 35 days postinfection. Phenotypic analysis of LNC was also carried out. No significant differences in thein vitroproliferation of LNC to VSG, to hsp70/BiP, or to Con A were detected between the breeds. In contrast, IFN-γ production in response to Con A was higher in Boran cattle at 35 days p.i. A reduction in the number of CD2+ and CD4+ T-cells and an increase in the percentage of B-cells, CD8+ T-cells, and γδ T-cells during infection in both N'Dama and Boran was revealed by cytofluorimetric analysis of lymph node cells.

AB - A comparison of T-cell-mediated immune responses in trypanotolerant N'Dama and susceptible Boran cattle during primary infection with tsetse-transmittedTrypanosoma congolensewas conducted to assess whether different patterns of T-cell activation occurred during trypanosome infection. Proliferation and IFN-γ synthesis in response to trypanosome antigens and to the mitogen Con A were measured in LNC before infection and 10 and 35 days postinfection. Phenotypic analysis of LNC was also carried out. No significant differences in thein vitroproliferation of LNC to VSG, to hsp70/BiP, or to Con A were detected between the breeds. In contrast, IFN-γ production in response to Con A was higher in Boran cattle at 35 days p.i. A reduction in the number of CD2+ and CD4+ T-cells and an increase in the percentage of B-cells, CD8+ T-cells, and γδ T-cells during infection in both N'Dama and Boran was revealed by cytofluorimetric analysis of lymph node cells.

KW - trypanosomiasis

KW - bovine

KW - Trypanosoma congolense

KW - T-cell proliferative responses

KW - IFN-gamma synthesis

KW - surface phenotype

UR - http://dx.doi.org/10.1006/expr.1996.0120

U2 - 10.1006/expr.1996.0120

DO - 10.1006/expr.1996.0120

M3 - Article

VL - 84

SP - 320

EP - 329

JO - Experimental Parasitology

T2 - Experimental Parasitology

JF - Experimental Parasitology

SN - 0014-4894

IS - 3

ER -