Transport of Sporosarcina pasteurii in sandstone and its significance for subsurface engineering technologies

Dominique J. Tobler, Mark O. Cuthbert, Vernon R. Phoenix

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

The development of microbially mediated technologies for subsurface remediation and rock engineering is steadily increasing; however, we are lacking experimental data and models to predict bacterial movement through rock matrices. Here, breakthrough curves (BTCs) were obtained to quantify the transport of the ureolytic bacterium, Sporosarcina pasteurii, through sandstone cores, as a function of core length (1.8-7.5cm), bacterial density (4×106 to 9×107cells/ml) and flow rate (5.8-17.5m/s). S. pasteurii was easily immobilised within the homogeneous sandstone matrix (>80%) in comparison to a packed sand column (<20%; under similar experimental conditions), and percentage recovery decreased almost linearly with increasing rock core length. Moreover, a decrease in bacterial density or flow rate enhanced bacterial retention. A numerical model based on 1D advection dispersion models used for unconsolidated sand was fitted to the BTC data obtained here for sandstone. Good agreement between data and model was obtained at shorter rock core lengths (<4cm), suggesting that physicochemical filtration processes are similar in homogeneous packed sand and sandstones at these lengths. Discrepancies were, however observed at longer core lengths and with varying flow rates, indicating that the attributes of consolidated rock might impact bacterial transport progressively more with increasing core length. Implications of these results on microbial mineralisation technologies currently being developed for sealing fluid paths in subsurface environment is discussed.

LanguageEnglish
Pages38-44
Number of pages7
JournalApplied Geochemistry
Volume42
Early online date18 Jan 2014
DOIs
Publication statusPublished - 31 Mar 2014
Externally publishedYes

Fingerprint

Engineering technology
Sandstone
Rocks
sandstone
engineering
Sand
rock
Flow rate
breakthrough curve
sand
matrix
Advection
sealing
Remediation
Numerical models
Bacteria
advection
remediation
mineralization
Recovery

Keywords

  • advection dispersion models
  • bacterial retentions
  • bacterial transport
  • break through curve
  • experimental conditions
  • subsurface environment
  • unconsolidated sands

Cite this

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title = "Transport of Sporosarcina pasteurii in sandstone and its significance for subsurface engineering technologies",
abstract = "The development of microbially mediated technologies for subsurface remediation and rock engineering is steadily increasing; however, we are lacking experimental data and models to predict bacterial movement through rock matrices. Here, breakthrough curves (BTCs) were obtained to quantify the transport of the ureolytic bacterium, Sporosarcina pasteurii, through sandstone cores, as a function of core length (1.8-7.5cm), bacterial density (4×106 to 9×107cells/ml) and flow rate (5.8-17.5m/s). S. pasteurii was easily immobilised within the homogeneous sandstone matrix (>80{\%}) in comparison to a packed sand column (<20{\%}; under similar experimental conditions), and percentage recovery decreased almost linearly with increasing rock core length. Moreover, a decrease in bacterial density or flow rate enhanced bacterial retention. A numerical model based on 1D advection dispersion models used for unconsolidated sand was fitted to the BTC data obtained here for sandstone. Good agreement between data and model was obtained at shorter rock core lengths (<4cm), suggesting that physicochemical filtration processes are similar in homogeneous packed sand and sandstones at these lengths. Discrepancies were, however observed at longer core lengths and with varying flow rates, indicating that the attributes of consolidated rock might impact bacterial transport progressively more with increasing core length. Implications of these results on microbial mineralisation technologies currently being developed for sealing fluid paths in subsurface environment is discussed.",
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Transport of Sporosarcina pasteurii in sandstone and its significance for subsurface engineering technologies. / Tobler, Dominique J.; Cuthbert, Mark O.; Phoenix, Vernon R.

In: Applied Geochemistry, Vol. 42, 31.03.2014, p. 38-44.

Research output: Contribution to journalArticle

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