Transcriptional analysis of pseudomonas aeruginosa Infected caenorhabditis elegans

Ashleigh Mackenzie, Lewis Stewart, Paul A Hoskisson, Nicholas P Tucker

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Here, we describe a protocol for the extraction of total RNA from C. elegans infected with P. aeruginosa. The protocol excludes P. aeruginosa cells that have not been ingested by the nematodes and yields total RNA that can be used for detection of transcripts from both host and pathogen.

Original languageEnglish
Title of host publicationPseudomonas Methods and Protocols
EditorsAlain Filloux, Juan-Luis Ramos
Place of PublicationNew York
PublisherSpringer
Pages607-612
Number of pages6
ISBN (Print)9781493904723
DOIs
Publication statusPublished - 2014

Publication series

NameMethods in Molecular Biology
PublisherSpringer
Volume1149
ISSN (Print)1064-3745

Keywords

  • transcriptional analysis
  • pseudomonas aeruginosa
  • caenorhabditis elegans

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