Transcriptional analysis of pseudomonas aeruginosa Infected caenorhabditis elegans

Ashleigh Mackenzie; Lewis Stewart; Paul A Hoskisson; Nicholas P Tucker

doi:10.1007/978-1-4939-0473-0_46

Transcriptional analysis of pseudomonas aeruginosa Infected caenorhabditis elegans

Ashleigh Mackenzie, Lewis Stewart, Paul A Hoskisson, Nicholas P Tucker

Strathclyde Institute Of Pharmacy And Biomedical Sciences

Research output: Chapter in Book/Report/Conference proceeding › Chapter

Abstract

Here, we describe a protocol for the extraction of total RNA from C. elegans infected with P. aeruginosa. The protocol excludes P. aeruginosa cells that have not been ingested by the nematodes and yields total RNA that can be used for detection of transcripts from both host and pathogen.

Original language	English
Title of host publication	Pseudomonas Methods and Protocols
Editors	Alain Filloux, Juan-Luis Ramos
Place of Publication	New York
Publisher	Springer
Pages	607-612
Number of pages	6
ISBN (Print)	9781493904723
DOIs	https://doi.org/10.1007/978-1-4939-0473-0_46
Publication status	Published - 2014

Publication series

Name	Methods in Molecular Biology
Publisher	Springer
Volume	1149
ISSN (Print)	1064-3745

Keywords

transcriptional analysis
pseudomonas aeruginosa
caenorhabditis elegans

Access to Document

10.1007/978-1-4939-0473-0_46

Comparative genomics of Pseudomonas aeruginosa
Nicholas Tucker (Contributor)
25 Jun 2012
Activity: Talk or presentation types › Invited talk

Cite this

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title = "Transcriptional analysis of pseudomonas aeruginosa Infected caenorhabditis elegans",

abstract = "Here, we describe a protocol for the extraction of total RNA from C. elegans infected with P. aeruginosa. The protocol excludes P. aeruginosa cells that have not been ingested by the nematodes and yields total RNA that can be used for detection of transcripts from both host and pathogen.",

keywords = "transcriptional analysis, pseudomonas aeruginosa, caenorhabditis elegans",

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Transcriptional analysis of pseudomonas aeruginosa Infected caenorhabditis elegans. / Mackenzie, Ashleigh; Stewart, Lewis; Hoskisson, Paul A et al.

Pseudomonas Methods and Protocols. ed. / Alain Filloux; Juan-Luis Ramos. New York : Springer, 2014. p. 607-612 (Methods in Molecular Biology; Vol. 1149).

Research output: Chapter in Book/Report/Conference proceeding › Chapter

TY - CHAP

T1 - Transcriptional analysis of pseudomonas aeruginosa Infected caenorhabditis elegans

AU - Mackenzie, Ashleigh

AU - Stewart, Lewis

AU - Hoskisson, Paul A

AU - Tucker, Nicholas P

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AB - Here, we describe a protocol for the extraction of total RNA from C. elegans infected with P. aeruginosa. The protocol excludes P. aeruginosa cells that have not been ingested by the nematodes and yields total RNA that can be used for detection of transcripts from both host and pathogen.

KW - transcriptional analysis

KW - pseudomonas aeruginosa

KW - caenorhabditis elegans

U2 - 10.1007/978-1-4939-0473-0_46

DO - 10.1007/978-1-4939-0473-0_46

M3 - Chapter

C2 - 24818936

SN - 9781493904723

T3 - Methods in Molecular Biology

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A2 - Ramos, Juan-Luis

PB - Springer

CY - New York

ER -

Transcriptional analysis of pseudomonas aeruginosa Infected caenorhabditis elegans

Abstract

Publication series

Keywords

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Comparative genomics of Pseudomonas aeruginosa

Cite this