Transcriptional analysis of pseudomonas aeruginosa Infected caenorhabditis elegans

Ashleigh Mackenzie, Lewis Stewart, Paul A Hoskisson, Nicholas P Tucker

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Here, we describe a protocol for the extraction of total RNA from C. elegans infected with P. aeruginosa. The protocol excludes P. aeruginosa cells that have not been ingested by the nematodes and yields total RNA that can be used for detection of transcripts from both host and pathogen.

LanguageEnglish
Title of host publicationPseudomonas Methods and Protocols
EditorsAlain Filloux, Juan-Luis Ramos
Place of PublicationNew York
PublisherSpringer
Pages607-612
Number of pages6
ISBN (Print)9781493904723
DOIs
Publication statusPublished - 2014

Publication series

NameMethods in Molecular Biology
PublisherSpringer
Volume1149
ISSN (Print)1064-3745

Fingerprint

Caenorhabditis elegans
Pseudomonas aeruginosa
RNA

Keywords

  • transcriptional analysis
  • pseudomonas aeruginosa
  • caenorhabditis elegans

Cite this

Mackenzie, A., Stewart, L., Hoskisson, P. A., & Tucker, N. P. (2014). Transcriptional analysis of pseudomonas aeruginosa Infected caenorhabditis elegans. In A. Filloux, & J-L. Ramos (Eds.), Pseudomonas Methods and Protocols (pp. 607-612). (Methods in Molecular Biology; Vol. 1149). New York: Springer. https://doi.org/10.1007/978-1-4939-0473-0_46
Mackenzie, Ashleigh ; Stewart, Lewis ; Hoskisson, Paul A ; Tucker, Nicholas P. / Transcriptional analysis of pseudomonas aeruginosa Infected caenorhabditis elegans. Pseudomonas Methods and Protocols. editor / Alain Filloux ; Juan-Luis Ramos. New York : Springer, 2014. pp. 607-612 (Methods in Molecular Biology).
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Mackenzie, A, Stewart, L, Hoskisson, PA & Tucker, NP 2014, Transcriptional analysis of pseudomonas aeruginosa Infected caenorhabditis elegans. in A Filloux & J-L Ramos (eds), Pseudomonas Methods and Protocols. Methods in Molecular Biology, vol. 1149, Springer, New York, pp. 607-612. https://doi.org/10.1007/978-1-4939-0473-0_46

Transcriptional analysis of pseudomonas aeruginosa Infected caenorhabditis elegans. / Mackenzie, Ashleigh; Stewart, Lewis; Hoskisson, Paul A; Tucker, Nicholas P.

Pseudomonas Methods and Protocols. ed. / Alain Filloux; Juan-Luis Ramos. New York : Springer, 2014. p. 607-612 (Methods in Molecular Biology; Vol. 1149).

Research output: Chapter in Book/Report/Conference proceedingChapter

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Mackenzie A, Stewart L, Hoskisson PA, Tucker NP. Transcriptional analysis of pseudomonas aeruginosa Infected caenorhabditis elegans. In Filloux A, Ramos J-L, editors, Pseudomonas Methods and Protocols. New York: Springer. 2014. p. 607-612. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-4939-0473-0_46