Tracking insulin glycation in real time by time-resolved emission spectroscopy

Li Hung C. Chung, David J. S. Birch, Vladislav Vyshemirsky, Maxim G. Ryadnov, Olaf J. Rolinski

Research output: Contribution to journalArticle

Abstract

The application of time-resolved fluorescence sensing to the study of heterogenic biomolecular systems remains challenging because of the complexity of the resulting photophysics. Measuring the time-resolved emission spectroscopy (TRES) spectra can provide a more informative alternative to the modeling of the fluorescence decay that is currently employed. Here, we demonstrate this approach by monitoring real-time changes in intrinsic insulin fluorescence by TRES as a straightforward probe to directly measure kinetics of insulin aggregation and glycation. Our findings hold promise for monitoring the storage of insulin and its application in the control of diabetes and may support the development of more effective therapeutics against amyloidosis.
Original languageEnglish
Pages (from-to)7812-7817
Number of pages6
JournalJournal of Physical Chemistry B
Volume123
Issue number37
Early online date23 Aug 2019
DOIs
Publication statusPublished - 19 Sep 2019

Keywords

  • time-resolved emission spectroscopy
  • TRES
  • insulin
  • glycation

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