Tracking insulin glycation in real time by time-resolved emission spectroscopy

Li Hung C. Chung; David J. S. Birch; Vladislav Vyshemirsky; Maxim G. Ryadnov; Olaf J. Rolinski

doi:10.1021/acs.jpcb.9b06363

Tracking insulin glycation in real time by time-resolved emission spectroscopy

Li Hung C. Chung, David J. S. Birch, Vladislav Vyshemirsky, Maxim G. Ryadnov, Olaf J. Rolinski

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Abstract

The application of time-resolved fluorescence sensing to the study of heterogenic biomolecular systems remains challenging because of the complexity of the resulting photophysics. Measuring the time-resolved emission spectroscopy (TRES) spectra can provide a more informative alternative to the modeling of the fluorescence decay that is currently employed. Here, we demonstrate this approach by monitoring real-time changes in intrinsic insulin fluorescence by TRES as a straightforward probe to directly measure kinetics of insulin aggregation and glycation. Our findings hold promise for monitoring the storage of insulin and its application in the control of diabetes and may support the development of more effective therapeutics against amyloidosis.

Original language	English
Pages (from-to)	7812-7817
Number of pages	6
Journal	Journal of Physical Chemistry B
Volume	123
Issue number	37
Early online date	23 Aug 2019
DOIs	https://doi.org/10.1021/acs.jpcb.9b06363
Publication status	Published - 19 Sept 2019

Keywords

time-resolved emission spectroscopy
TRES
insulin
glycation

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Chung-etal-JPCB-2019-Tracking-insulin-glycation-in-real-timeAccepted author manuscript, 4.65 MB

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title = "Tracking insulin glycation in real time by time-resolved emission spectroscopy",

abstract = "The application of time-resolved fluorescence sensing to the study of heterogenic biomolecular systems remains challenging because of the complexity of the resulting photophysics. Measuring the time-resolved emission spectroscopy (TRES) spectra can provide a more informative alternative to the modeling of the fluorescence decay that is currently employed. Here, we demonstrate this approach by monitoring real-time changes in intrinsic insulin fluorescence by TRES as a straightforward probe to directly measure kinetics of insulin aggregation and glycation. Our findings hold promise for monitoring the storage of insulin and its application in the control of diabetes and may support the development of more effective therapeutics against amyloidosis.",

keywords = "time-resolved emission spectroscopy, TRES, insulin, glycation",

author = "Chung, {Li Hung C.} and Birch, {David J. S.} and Vladislav Vyshemirsky and Ryadnov, {Maxim G.} and Rolinski, {Olaf J.}",

year = "2019",

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T1 - Tracking insulin glycation in real time by time-resolved emission spectroscopy

AU - Chung, Li Hung C.

AU - Birch, David J. S.

AU - Vyshemirsky, Vladislav

AU - Ryadnov, Maxim G.

AU - Rolinski, Olaf J.

PY - 2019/9/19

Y1 - 2019/9/19

N2 - The application of time-resolved fluorescence sensing to the study of heterogenic biomolecular systems remains challenging because of the complexity of the resulting photophysics. Measuring the time-resolved emission spectroscopy (TRES) spectra can provide a more informative alternative to the modeling of the fluorescence decay that is currently employed. Here, we demonstrate this approach by monitoring real-time changes in intrinsic insulin fluorescence by TRES as a straightforward probe to directly measure kinetics of insulin aggregation and glycation. Our findings hold promise for monitoring the storage of insulin and its application in the control of diabetes and may support the development of more effective therapeutics against amyloidosis.

AB - The application of time-resolved fluorescence sensing to the study of heterogenic biomolecular systems remains challenging because of the complexity of the resulting photophysics. Measuring the time-resolved emission spectroscopy (TRES) spectra can provide a more informative alternative to the modeling of the fluorescence decay that is currently employed. Here, we demonstrate this approach by monitoring real-time changes in intrinsic insulin fluorescence by TRES as a straightforward probe to directly measure kinetics of insulin aggregation and glycation. Our findings hold promise for monitoring the storage of insulin and its application in the control of diabetes and may support the development of more effective therapeutics against amyloidosis.

KW - time-resolved emission spectroscopy

KW - TRES

KW - insulin

KW - glycation

U2 - 10.1021/acs.jpcb.9b06363

DO - 10.1021/acs.jpcb.9b06363

M3 - Article

SN - 1520-6106

VL - 123

SP - 7812

EP - 7817

JO - Journal of Physical Chemistry B

JF - Journal of Physical Chemistry B

IS - 37

ER -

Tracking insulin glycation in real time by time-resolved emission spectroscopy

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Keywords

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