Towards automatic cell identification in DIC microscopy

D. Young, C. A. Glasbey, A. J. Gray, N. J. Martin

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

A general method is proposed for constructing templates of cells in differential interference contrast (DIC) microscopy. This takes account of the optics which generate DIC images, and is applicable to both transparent and semi-transparent cells of simple and complex shapes. Then, a template matching methodology is presented, which uses fast Fourier transforms to fit templates of a range of sizes and orientations to images. For illustration, this is used to automatically identify and measure individual Candida yeast cells in clusters.

Original languageEnglish
Pages (from-to)186-193
Number of pages8
JournalJournal of Microscopy
Volume192
Issue number2
DOIs
Publication statusPublished - 1 Nov 1998

Keywords

  • automated microscopy
  • differential interference contrast microscopy
  • templates

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