Towards a characterisation of the wild legume bitter vetch (Lathyrus linifolius L. (Reichard) Bässler): heteromorphic seed germination, root nodule structure and N-fixing rhizobial symbionts

E. Dello Jacovo, T. A. Valentine, M. Maluk, P. Toorop, L. Lopez del Egido, N. Frachon, G. Kenicer, L. Park, M. Goff, V. A. Ferro, C. Bonomi, E. K. James, P. P. M. Iannetta

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Lathyrus linifolius L. (Reichard) Bässler (bitter vetch) is a fabaceous nitrogen (N) fixing species. A coloniser of low nutrient (N) soils it supports biodiversity such as key moth and butterfly species and its roots are known for their organoleptic and claimed therapeutic properties. Thus, the species has high potential for restoration, conservation, novel cropping and as model species. The latter owing to its genetic synteny with important pulse crops. However, regeneration and functional attributes of L. linifolius remain to be characterised. Seeds of L. linifolius were characterised using physical, colourimetric and chemical data. Ultrastructural and functional characterisation of the N fixing root nodules included immunolabelling with nifH-protein antibodies (recognising the N fixing enzyme, nitrogenase). Endosymbiotic bacteria were isolated from the root nodules and characterised phylogenetically using 16S rRNA, nodA and nodD gene sequeneces. L. linifolius yielded hetermorphic seeds of distinct colour classes: green and brown. Seed morphotypes had similar carbon:N ratios and were equally germinable (ca. 90%) after scarification at differing optimal temperatures (16 and 20°C, respectively). Brown seeds were larger and comprised a larger proportion of the seed batch (69%). L. linifolius root nodules appeared indeterminate in structure, effective (capable of fixing atmospheric N) and accommodated strains with high similarity to Rhizobium leguminosarum biovar viciae. The findings and rhizobial isolates have potential application for ecological restoration and horticulture using native seeds. Also, the data and rhizobial resources have potential application in comparative and functional studies with related and socio-economically important crops such as Pisum, Lens and Vicia.

LanguageEnglish
Number of pages10
JournalPlant Biology
Early online date18 Aug 2018
DOIs
Publication statusE-pub ahead of print - 18 Aug 2018

Fingerprint

Lathyrus
root nodules
symbiont
symbionts
germination
seed germination
legumes
seed
seeds
Rhizobium leguminosarum bv. viciae
scarification
Pisum
crop
horticulture
medicinal properties
ecological restoration
Vicia
nitrogenase
morphotype
crops

Keywords

  • bitter vetch
  • Lathyrus linifolius L.
  • legume root noodles
  • Rhizobium
  • seed germination

Cite this

Jacovo, E. Dello ; Valentine, T. A. ; Maluk, M. ; Toorop, P. ; Lopez del Egido, L. ; Frachon, N. ; Kenicer, G. ; Park, L. ; Goff, M. ; Ferro, V. A. ; Bonomi, C. ; James, E. K. ; Iannetta, P. P. M. / Towards a characterisation of the wild legume bitter vetch (Lathyrus linifolius L. (Reichard) Bässler) : heteromorphic seed germination, root nodule structure and N-fixing rhizobial symbionts. In: Plant Biology. 2018.
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abstract = "Lathyrus linifolius L. (Reichard) B{\"a}ssler (bitter vetch) is a fabaceous nitrogen (N) fixing species. A coloniser of low nutrient (N) soils it supports biodiversity such as key moth and butterfly species and its roots are known for their organoleptic and claimed therapeutic properties. Thus, the species has high potential for restoration, conservation, novel cropping and as model species. The latter owing to its genetic synteny with important pulse crops. However, regeneration and functional attributes of L. linifolius remain to be characterised. Seeds of L. linifolius were characterised using physical, colourimetric and chemical data. Ultrastructural and functional characterisation of the N fixing root nodules included immunolabelling with nifH-protein antibodies (recognising the N fixing enzyme, nitrogenase). Endosymbiotic bacteria were isolated from the root nodules and characterised phylogenetically using 16S rRNA, nodA and nodD gene sequeneces. L. linifolius yielded hetermorphic seeds of distinct colour classes: green and brown. Seed morphotypes had similar carbon:N ratios and were equally germinable (ca. 90{\%}) after scarification at differing optimal temperatures (16 and 20°C, respectively). Brown seeds were larger and comprised a larger proportion of the seed batch (69{\%}). L. linifolius root nodules appeared indeterminate in structure, effective (capable of fixing atmospheric N) and accommodated strains with high similarity to Rhizobium leguminosarum biovar viciae. The findings and rhizobial isolates have potential application for ecological restoration and horticulture using native seeds. Also, the data and rhizobial resources have potential application in comparative and functional studies with related and socio-economically important crops such as Pisum, Lens and Vicia.",
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Towards a characterisation of the wild legume bitter vetch (Lathyrus linifolius L. (Reichard) Bässler) : heteromorphic seed germination, root nodule structure and N-fixing rhizobial symbionts. / Jacovo, E. Dello; Valentine, T. A.; Maluk, M.; Toorop, P.; Lopez del Egido, L.; Frachon, N.; Kenicer, G.; Park, L.; Goff, M.; Ferro, V. A.; Bonomi, C.; James, E. K.; Iannetta, P. P. M.

In: Plant Biology, 18.08.2018.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Towards a characterisation of the wild legume bitter vetch (Lathyrus linifolius L. (Reichard) Bässler)

T2 - Plant Biology

AU - Jacovo, E. Dello

AU - Valentine, T. A.

AU - Maluk, M.

AU - Toorop, P.

AU - Lopez del Egido, L.

AU - Frachon, N.

AU - Kenicer, G.

AU - Park, L.

AU - Goff, M.

AU - Ferro, V. A.

AU - Bonomi, C.

AU - James, E. K.

AU - Iannetta, P. P. M.

N1 - This article is protected by copyright. All rights reserved.

PY - 2018/8/18

Y1 - 2018/8/18

N2 - Lathyrus linifolius L. (Reichard) Bässler (bitter vetch) is a fabaceous nitrogen (N) fixing species. A coloniser of low nutrient (N) soils it supports biodiversity such as key moth and butterfly species and its roots are known for their organoleptic and claimed therapeutic properties. Thus, the species has high potential for restoration, conservation, novel cropping and as model species. The latter owing to its genetic synteny with important pulse crops. However, regeneration and functional attributes of L. linifolius remain to be characterised. Seeds of L. linifolius were characterised using physical, colourimetric and chemical data. Ultrastructural and functional characterisation of the N fixing root nodules included immunolabelling with nifH-protein antibodies (recognising the N fixing enzyme, nitrogenase). Endosymbiotic bacteria were isolated from the root nodules and characterised phylogenetically using 16S rRNA, nodA and nodD gene sequeneces. L. linifolius yielded hetermorphic seeds of distinct colour classes: green and brown. Seed morphotypes had similar carbon:N ratios and were equally germinable (ca. 90%) after scarification at differing optimal temperatures (16 and 20°C, respectively). Brown seeds were larger and comprised a larger proportion of the seed batch (69%). L. linifolius root nodules appeared indeterminate in structure, effective (capable of fixing atmospheric N) and accommodated strains with high similarity to Rhizobium leguminosarum biovar viciae. The findings and rhizobial isolates have potential application for ecological restoration and horticulture using native seeds. Also, the data and rhizobial resources have potential application in comparative and functional studies with related and socio-economically important crops such as Pisum, Lens and Vicia.

AB - Lathyrus linifolius L. (Reichard) Bässler (bitter vetch) is a fabaceous nitrogen (N) fixing species. A coloniser of low nutrient (N) soils it supports biodiversity such as key moth and butterfly species and its roots are known for their organoleptic and claimed therapeutic properties. Thus, the species has high potential for restoration, conservation, novel cropping and as model species. The latter owing to its genetic synteny with important pulse crops. However, regeneration and functional attributes of L. linifolius remain to be characterised. Seeds of L. linifolius were characterised using physical, colourimetric and chemical data. Ultrastructural and functional characterisation of the N fixing root nodules included immunolabelling with nifH-protein antibodies (recognising the N fixing enzyme, nitrogenase). Endosymbiotic bacteria were isolated from the root nodules and characterised phylogenetically using 16S rRNA, nodA and nodD gene sequeneces. L. linifolius yielded hetermorphic seeds of distinct colour classes: green and brown. Seed morphotypes had similar carbon:N ratios and were equally germinable (ca. 90%) after scarification at differing optimal temperatures (16 and 20°C, respectively). Brown seeds were larger and comprised a larger proportion of the seed batch (69%). L. linifolius root nodules appeared indeterminate in structure, effective (capable of fixing atmospheric N) and accommodated strains with high similarity to Rhizobium leguminosarum biovar viciae. The findings and rhizobial isolates have potential application for ecological restoration and horticulture using native seeds. Also, the data and rhizobial resources have potential application in comparative and functional studies with related and socio-economically important crops such as Pisum, Lens and Vicia.

KW - bitter vetch

KW - Lathyrus linifolius L.

KW - legume root noodles

KW - Rhizobium

KW - seed germination

UR - https://onlinelibrary.wiley.com/journal/14388677

U2 - 10.1111/plb.12902

DO - 10.1111/plb.12902

M3 - Article

JO - Plant Biology

JF - Plant Biology

SN - 1435-8603

ER -