Tolerant t cells display impaired trafficking ability

V. Mirenda, O.R. Millington, R.I. Lechler, D. Scott, M.P. Mernandez-Fuentes, J. Read, P.H. Tan, A.J. George, P. Garside, F.M. Marelli-Berg

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Based on our previous observation that anergic T lymphocytes lose their migratory ability in vitro, we have proposed that anergic T cells are retained in the site where they have been generated to exert their regulatory function. In this study we have analyzed T lymphocyte trafficking and motility following the induction of tolerance in vivo. In a model of non-deletional negative vaccination to xenoantigens in which dendritic cells (DC) localize to specific lymphoid sites depending on the route of administration, tolerant T cells remained localized in the lymph nodes colonized by tolerogenic DC, while primed T cells could traffic efficiently. Using an oral tolerance model that enables the ‘tracking’ of ovalbumin-specific TCR-transgenic T cells, we confirmed that T cells lose the ability to migrate through syngeneic endothelial cell monolayers following tolerance induction in vivo. Finally, we show that tolerant T cells (both in vitro and ex vivo) can inhibit migration of responsive T cells in an antigen-independent manner. Thus, hyporesponsive T cells localize at the site of tolerance induction in vivo, where they exert their anti-inflammatory properties. In physiological terms, this effect is likely to render immunoregulation a more efficient and controllable event.

Original languageEnglish
Pages (from-to)2146-2156
Number of pages11
JournalEuropean Journal of Immunology
Volume35
Issue number7
DOIs
Publication statusPublished - 2005

Keywords

  • T cells
  • anargy
  • tolerance
  • trafficking

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    Mirenda, V., Millington, O. R., Lechler, R. I., Scott, D., Mernandez-Fuentes, M. P., Read, J., ... Marelli-Berg, F. M. (2005). Tolerant t cells display impaired trafficking ability. European Journal of Immunology, 35(7), 2146-2156. https://doi.org/10.1002/eji.200425823