ToF-SIMS analysis of ocular tissues reveals biochemical differentiation and drug distribution

Jenifer Mains, Clive Wilson, Andrew Urquhart

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Time-of-flight secondary ion mass spectrometry (ToF-SIMS) was used to obtain mass spectra from three ocular tissues, the lens, the vitreous and the retina. All three tissues were extracted from control ovine eyes and ovine eyes treated with model drug. To identify variations in surface biochemistry of each ocular tissue, principal component analysis (PCA) was applied to ToF-SIMS data. Interesting physiological differences in Na(+) and K(+) distribution were shown across the three tissue types, with other elements including Ca(2+) and Fe(2+) distribution also detected. In addition to the identification of small molecules and smaller molecular fragments, larger molecules such as phosphocholine were also detected. The ToF-SIMS data were also used to identify the presence of a model drug compound (amitriptyline--chosen as a generic drug structure) within all three ocular tissues, with model drug detected predominantly across the vitreous tissue samples. This study demonstrates that PCA can be successfully applied to ToF-SIMS data from different ocular tissues and highlights the potential of coupling multivariate statistics with surface analytical techniques to gain a greater understanding of the biochemical composition of tissues and the distribution of pharmaceutically active small molecules within these tissues.
Original languageEnglish
Pages (from-to)328–333
Number of pages6
JournalEuropean Journal of Pharmaceutics and Biopharmaceutics
Volume79
Issue number2
Early online date21 Apr 2011
DOIs
Publication statusPublished - 1 Oct 2011

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Secondary Ion Mass Spectrometry
Pharmaceutical Preparations
Principal Component Analysis
Sheep
Generic Drugs
Crystalline Lens
Amitriptyline
Phosphorylcholine
Tissue Distribution
Biochemistry
Retina

Keywords

  • ocular tissues
  • mass spectrometry
  • phosphocholine
  • eyes
  • TIC - Bionanotechnology

Cite this

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title = "ToF-SIMS analysis of ocular tissues reveals biochemical differentiation and drug distribution",
abstract = "Time-of-flight secondary ion mass spectrometry (ToF-SIMS) was used to obtain mass spectra from three ocular tissues, the lens, the vitreous and the retina. All three tissues were extracted from control ovine eyes and ovine eyes treated with model drug. To identify variations in surface biochemistry of each ocular tissue, principal component analysis (PCA) was applied to ToF-SIMS data. Interesting physiological differences in Na(+) and K(+) distribution were shown across the three tissue types, with other elements including Ca(2+) and Fe(2+) distribution also detected. In addition to the identification of small molecules and smaller molecular fragments, larger molecules such as phosphocholine were also detected. The ToF-SIMS data were also used to identify the presence of a model drug compound (amitriptyline--chosen as a generic drug structure) within all three ocular tissues, with model drug detected predominantly across the vitreous tissue samples. This study demonstrates that PCA can be successfully applied to ToF-SIMS data from different ocular tissues and highlights the potential of coupling multivariate statistics with surface analytical techniques to gain a greater understanding of the biochemical composition of tissues and the distribution of pharmaceutically active small molecules within these tissues.",
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ToF-SIMS analysis of ocular tissues reveals biochemical differentiation and drug distribution. / Mains, Jenifer; Wilson, Clive; Urquhart, Andrew.

In: European Journal of Pharmaceutics and Biopharmaceutics, Vol. 79, No. 2, 01.10.2011, p. 328–333.

Research output: Contribution to journalArticle

TY - JOUR

T1 - ToF-SIMS analysis of ocular tissues reveals biochemical differentiation and drug distribution

AU - Mains, Jenifer

AU - Wilson, Clive

AU - Urquhart, Andrew

N1 - Copyright © 2011 Elsevier B.V. All rights reserved.

PY - 2011/10/1

Y1 - 2011/10/1

N2 - Time-of-flight secondary ion mass spectrometry (ToF-SIMS) was used to obtain mass spectra from three ocular tissues, the lens, the vitreous and the retina. All three tissues were extracted from control ovine eyes and ovine eyes treated with model drug. To identify variations in surface biochemistry of each ocular tissue, principal component analysis (PCA) was applied to ToF-SIMS data. Interesting physiological differences in Na(+) and K(+) distribution were shown across the three tissue types, with other elements including Ca(2+) and Fe(2+) distribution also detected. In addition to the identification of small molecules and smaller molecular fragments, larger molecules such as phosphocholine were also detected. The ToF-SIMS data were also used to identify the presence of a model drug compound (amitriptyline--chosen as a generic drug structure) within all three ocular tissues, with model drug detected predominantly across the vitreous tissue samples. This study demonstrates that PCA can be successfully applied to ToF-SIMS data from different ocular tissues and highlights the potential of coupling multivariate statistics with surface analytical techniques to gain a greater understanding of the biochemical composition of tissues and the distribution of pharmaceutically active small molecules within these tissues.

AB - Time-of-flight secondary ion mass spectrometry (ToF-SIMS) was used to obtain mass spectra from three ocular tissues, the lens, the vitreous and the retina. All three tissues were extracted from control ovine eyes and ovine eyes treated with model drug. To identify variations in surface biochemistry of each ocular tissue, principal component analysis (PCA) was applied to ToF-SIMS data. Interesting physiological differences in Na(+) and K(+) distribution were shown across the three tissue types, with other elements including Ca(2+) and Fe(2+) distribution also detected. In addition to the identification of small molecules and smaller molecular fragments, larger molecules such as phosphocholine were also detected. The ToF-SIMS data were also used to identify the presence of a model drug compound (amitriptyline--chosen as a generic drug structure) within all three ocular tissues, with model drug detected predominantly across the vitreous tissue samples. This study demonstrates that PCA can be successfully applied to ToF-SIMS data from different ocular tissues and highlights the potential of coupling multivariate statistics with surface analytical techniques to gain a greater understanding of the biochemical composition of tissues and the distribution of pharmaceutically active small molecules within these tissues.

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