Tissue engineered scaffolds for mimetic autografts

Juan Antonio Romero-Torrecilla, Luis Riera, José Valdés-Fernández, Tania López-Martínez, Purificación Ripalda-Cemboráin, Vineetha Jayawarna, Peter Childs, Manuel Salmeron-Sanchez, Felipe Prósper-Cardoso, Froilán Granero-Moltó

Research output: Contribution to journalConference Contributionpeer-review

4 Downloads (Pure)


Introduction: Despite its regenerative capacity, bone healing can be compromised, leading to delayed fracture regeneration and nonunion. Due to the scarcity of bone tissue that can be used as autograft, novel tissue engineering strategies arise as a promising solution by using biocompatible materials.

Methods: Our objective is the development of engineered autografts capable of efficiently treat fracture nonunion. For this purpose, we designed polycaprolactone (PCL) autografts surrounded by a porous membrane mimicking periosteum. To assess their regenerative capacity, these scaffolds were tested in critical size femur defect for ten weeks carrying out μCT and histological analysis. Additionally, we are focusing on the generation of PCL biocomposites, such as poly ethyl-acrylate (PEA) covered PCL membranes which can enhance morphogen functionalization, reducing the effective BMP dose.

Results: At the mCT level, structural mimetic PCL scaffolds, showed no significant difference in bone healing (Empty group, 11.47±4.93 mm3; MA, 14.95±3.09 mm3, p=0.1711). Histological analysis demonstrates that MEW PCL mimicking periosteum enhances bone growth, but insufficient for successful healing. However, once functionalized with PEA and BMP-2, these implants showed highly improved regeneration (CTL group, 11,47±4,93 mm3; BMP-2 group, 49,24±13,20 mm3, p = 0.0001). Figure 1. These implants were loaded with BMP-2 solutions previously studied in vitro to estimate morphogen dose, which resulted in 55.64±14.83 ng (n=6).

Conclusions and discussion: In conclusion, PEA functionalized mimetic autografts show an important increase in bone healing, enhancing BMP-2 effects, which provide representative regeneration with a 100 folds lower dose than typically described in literature.
Original languageEnglish
Article numberP036
Pages (from-to)11
Number of pages1
JournalBone Reports
Issue number100386
Early online date22 Oct 2020
Publication statusPublished - 22 Oct 2020
EventECTS 2020 - Virtual
Duration: 22 Oct 202224 Oct 2022


  • tissue engineered scaffolds
  • mimetic autografts
  • polycaprolactone (PCL) autografts
  • bone healing


Dive into the research topics of 'Tissue engineered scaffolds for mimetic autografts'. Together they form a unique fingerprint.

Cite this