TiO₂-coated electrodes for pulsed electric field treatment of microorganisms

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Abstract

Pulsed electric fields (PEF) can cause irreversible damage to bio-membranes and may result in inactivation of microorganisms. The aim of this paper is to investigate the PEF treatment of the yeast Saccharomyces cerevisiae, using a novel treatment cell with parallel-plane electrodes coated with a 2 µm thin TiO2 film. Two different PEF waveforms, square and exponential, with magnitudes of 67 kV/cm and 80 kV/cm, were used in this study. The efficacy of the PEF treatment was assessed by comparison of the surviving treated and untreated yeast populations, and it was shown that a treatment cell with TiO2-coated electrodes can be successfully used for the PEF treatment of microorganisms: 3-log10 reduction in the yeast population was achieved with 100 impulses. The energy efficacy of the PEF process in the proposed treatment cell has been compared with the energy losses in the PEF treatment cell with uncoated, conductive electrodes. It is shown that the electrodes coated with TiO2 provide better performance as compared with the traditional uncoated electrodes.
LanguageEnglish
Pages2121-2128
Number of pages8
JournalIEEE Transactions on Plasma Science
Volume44
Issue number10
Early online date1 Jun 2016
DOIs
Publication statusPublished - 31 Oct 2016

Fingerprint

microorganisms
Microorganisms
Electric fields
Electrodes
electrodes
electric fields
Yeast
yeast
cells
saccharomyces
deactivation
impulses
Energy dissipation
waveforms
energy dissipation
membranes
damage
Membranes
Thin films
causes

Keywords

  • inactivation of microorganisms
  • pulsed electric field
  • yeast
  • PEF inactivation

Cite this

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title = "TiO₂-coated electrodes for pulsed electric field treatment of microorganisms",
abstract = "Pulsed electric fields (PEF) can cause irreversible damage to bio-membranes and may result in inactivation of microorganisms. The aim of this paper is to investigate the PEF treatment of the yeast Saccharomyces cerevisiae, using a novel treatment cell with parallel-plane electrodes coated with a 2 µm thin TiO2 film. Two different PEF waveforms, square and exponential, with magnitudes of 67 kV/cm and 80 kV/cm, were used in this study. The efficacy of the PEF treatment was assessed by comparison of the surviving treated and untreated yeast populations, and it was shown that a treatment cell with TiO2-coated electrodes can be successfully used for the PEF treatment of microorganisms: 3-log10 reduction in the yeast population was achieved with 100 impulses. The energy efficacy of the PEF process in the proposed treatment cell has been compared with the energy losses in the PEF treatment cell with uncoated, conductive electrodes. It is shown that the electrodes coated with TiO2 provide better performance as compared with the traditional uncoated electrodes.",
keywords = "inactivation of microorganisms, pulsed electric field, yeast, PEF inactivation",
author = "Si Qin and Timoshkin, {Igor V.} and Michelle MacLean and MacGregor, {Scott J.} and Wilson, {Mark P.} and Given, {Martin J.} and Tao Wang and Anderson, {John G.}",
note = "Published as part of a special issue, {"}Pulsed Power Science & Technology{"}. (c) 2016 IEEE. Personal use of this material is permitted. Permission from IEEE must be obtained for all other users, including reprinting/ republishing this material for advertising or promotional purposes, creating new collective works for resale or redistribution to servers or lists, or reuse of any copyrighted components of this work in other works.",
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AU - Qin, Si

AU - Timoshkin, Igor V.

AU - MacLean, Michelle

AU - MacGregor, Scott J.

AU - Wilson, Mark P.

AU - Given, Martin J.

AU - Wang, Tao

AU - Anderson, John G.

N1 - Published as part of a special issue, "Pulsed Power Science & Technology". (c) 2016 IEEE. Personal use of this material is permitted. Permission from IEEE must be obtained for all other users, including reprinting/ republishing this material for advertising or promotional purposes, creating new collective works for resale or redistribution to servers or lists, or reuse of any copyrighted components of this work in other works.

PY - 2016/10/31

Y1 - 2016/10/31

N2 - Pulsed electric fields (PEF) can cause irreversible damage to bio-membranes and may result in inactivation of microorganisms. The aim of this paper is to investigate the PEF treatment of the yeast Saccharomyces cerevisiae, using a novel treatment cell with parallel-plane electrodes coated with a 2 µm thin TiO2 film. Two different PEF waveforms, square and exponential, with magnitudes of 67 kV/cm and 80 kV/cm, were used in this study. The efficacy of the PEF treatment was assessed by comparison of the surviving treated and untreated yeast populations, and it was shown that a treatment cell with TiO2-coated electrodes can be successfully used for the PEF treatment of microorganisms: 3-log10 reduction in the yeast population was achieved with 100 impulses. The energy efficacy of the PEF process in the proposed treatment cell has been compared with the energy losses in the PEF treatment cell with uncoated, conductive electrodes. It is shown that the electrodes coated with TiO2 provide better performance as compared with the traditional uncoated electrodes.

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