Time-resolved optical Kerr-effect spectroscopy of low-frequency dynamics in Di-L-alanine, poly-L-alanine, and lysozyme in solution

Gerard Giraud, Klaas Wynne

Research output: Contribution to journalArticle

49 Citations (Scopus)

Abstract

The low-frequency spectra of peptides and proteins in solution have been investigated with optical heterodyne-detected Raman-induced Kerr-effect spectroscopy. Spectra were obtained for di-L-alanine ALA(2) and poly-L-alanine (PLA) in dichloroacetic acid solution. The conformational dependence of those spectra at low frequency has been analyzed. ALA(2) displays a band centered at 50 cm-1, whereas the -helical PLA shows two shoulders at 60 and 140 cm-1. The similarity of the spectral features observed in PLA to those in water can be explained by analogous acoustic translational modes in the hydrogen network of the PLA -helix. The mostly -helical protein lysozyme in aqueous solution has also been investigated and showed significantly more structure with modes at 10, 35, 73, 106, and 164 cm-1.
Original languageEnglish
Pages (from-to)12110-12111
Number of pages1
JournalJournal of Chemical Physics
Volume124
Issue number41
DOIs
Publication statusPublished - 21 Sep 2002

Keywords

  • Kerr-effect spectroscopy
  • low-frequency dynamics
  • Di-L-alanine
  • poly-L-alanine
  • lysozyme
  • nanoscience

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