Time-lapse measurement of single-cell response to nanomaterial: a microfluidic approach

C. A. Cunha-Matos; O. M. Millington; A. W. Wark; M. Zagnoni

Time-lapse measurement of single-cell response to nanomaterial: a microfluidic approach

C. A. Cunha-Matos, O. M. Millington, A. W. Wark, M. Zagnoni

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution book

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Abstract

This work presents the successful application of a single-cell microfluidic platform for high-throughput, real-time screening of nanoparticle-cell interactions. Taking vaccine delivery as a proof-of-concept application, ovalbumin-conjugated gold nanorods were produced and controllably delivered to primary dendritic cells within the device. Time-lapse imaging enabled monitoring of hundreds of single-cells during exposure to a range of concentrations of nanoparticle conjugates and simultaneous quantification of specific cellular functions. This integrated system provides throughput and statistical data comparable to that obtained with flow cytometry but also offers a novel approach to determine the dynamics of nanoparticle-cell interactions and nanoparticle-mediated antigen delivery with single-cell resolution.

Original language	English
Title of host publication	20th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2016)
Place of Publication	San Diego, CA
Pages	377-378
Number of pages	2
Publication status	Published - 9 Oct 2016
Event	20th International Conference on Miniaturized Systems for Chemistry and Life Sciences - Convention Center Dublin, Dublin, Ireland Duration: 9 Oct 2016 → 13 Oct 2016 http://www.microtas2016.org/

Conference

Conference	20th International Conference on Miniaturized Systems for Chemistry and Life Sciences
Abbreviated title	MicroTas 2016
Country	Ireland
City	Dublin
Period	9/10/16 → 13/10/16
Internet address	http://www.microtas2016.org/

Keywords

single-cell
gold nanorod
nano vaccine
high throughput assay

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Additional Sponsorship - University Of Strathclyde
Littlejohn, D.
EPSRC (Engineering and Physical Sciences Research Council)
1/07/11 → 30/06/12
Project: Research

Cite this

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title = "Time-lapse measurement of single-cell response to nanomaterial: a microfluidic approach",

abstract = "This work presents the successful application of a single-cell microfluidic platform for high-throughput, real-time screening of nanoparticle-cell interactions. Taking vaccine delivery as a proof-of-concept application, ovalbumin-conjugated gold nanorods were produced and controllably delivered to primary dendritic cells within the device. Time-lapse imaging enabled monitoring of hundreds of single-cells during exposure to a range of concentrations of nanoparticle conjugates and simultaneous quantification of specific cellular functions. This integrated system provides throughput and statistical data comparable to that obtained with flow cytometry but also offers a novel approach to determine the dynamics of nanoparticle-cell interactions and nanoparticle-mediated antigen delivery with single-cell resolution. ",

keywords = "single-cell, gold nanorod, nano vaccine, high throughput assay",

author = "Cunha-Matos, {C. A.} and Millington, {O. M.} and Wark, {A. W.} and M. Zagnoni",

year = "2016",

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booktitle = "20th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2016)",

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Cunha-Matos, CA, Millington, OM, Wark, AW & Zagnoni, M 2016, Time-lapse measurement of single-cell response to nanomaterial: a microfluidic approach. in 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2016). San Diego, CA, pp. 377-378, 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, Dublin, Ireland, 9/10/16.

Time-lapse measurement of single-cell response to nanomaterial : a microfluidic approach. / Cunha-Matos, C. A.; Millington, O. M.; Wark, A. W.; Zagnoni, M.

20th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2016). San Diego, CA, 2016. p. 377-378.

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution book

TY - GEN

T1 - Time-lapse measurement of single-cell response to nanomaterial

T2 - 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences

AU - Cunha-Matos, C. A.

AU - Millington, O. M.

AU - Wark, A. W.

AU - Zagnoni, M.

PY - 2016/10/9

Y1 - 2016/10/9

N2 - This work presents the successful application of a single-cell microfluidic platform for high-throughput, real-time screening of nanoparticle-cell interactions. Taking vaccine delivery as a proof-of-concept application, ovalbumin-conjugated gold nanorods were produced and controllably delivered to primary dendritic cells within the device. Time-lapse imaging enabled monitoring of hundreds of single-cells during exposure to a range of concentrations of nanoparticle conjugates and simultaneous quantification of specific cellular functions. This integrated system provides throughput and statistical data comparable to that obtained with flow cytometry but also offers a novel approach to determine the dynamics of nanoparticle-cell interactions and nanoparticle-mediated antigen delivery with single-cell resolution.

AB - This work presents the successful application of a single-cell microfluidic platform for high-throughput, real-time screening of nanoparticle-cell interactions. Taking vaccine delivery as a proof-of-concept application, ovalbumin-conjugated gold nanorods were produced and controllably delivered to primary dendritic cells within the device. Time-lapse imaging enabled monitoring of hundreds of single-cells during exposure to a range of concentrations of nanoparticle conjugates and simultaneous quantification of specific cellular functions. This integrated system provides throughput and statistical data comparable to that obtained with flow cytometry but also offers a novel approach to determine the dynamics of nanoparticle-cell interactions and nanoparticle-mediated antigen delivery with single-cell resolution.

KW - single-cell

KW - gold nanorod

KW - nano vaccine

KW - high throughput assay

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UR - http://microtas2016.org/

M3 - Conference contribution book

SN - 9781510834163

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EP - 378

BT - 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2016)

CY - San Diego, CA

Y2 - 9 October 2016 through 13 October 2016

ER -

Time-lapse measurement of single-cell response to nanomaterial: a microfluidic approach

Abstract

Conference

Keywords

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Additional Sponsorship - University Of Strathclyde

Cite this