Time-lapse measurement of single-cell response to nanomaterial: a microfluidic approach

C. A. Cunha-Matos, O. M. Millington, A. W. Wark, M. Zagnoni

Research output: Chapter in Book/Report/Conference proceedingConference contribution book

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Abstract

This work presents the successful application of a single-cell microfluidic platform for high-throughput, real-time screening of nanoparticle-cell interactions. Taking vaccine delivery as a proof-of-concept application, ovalbumin-conjugated gold nanorods were produced and controllably delivered to primary dendritic cells within the device. Time-lapse imaging enabled monitoring of hundreds of single-cells during exposure to a range of concentrations of nanoparticle conjugates and simultaneous quantification of specific cellular functions. This integrated system provides throughput and statistical data comparable to that obtained with flow cytometry but also offers a novel approach to determine the dynamics of nanoparticle-cell interactions and nanoparticle-mediated antigen delivery with single-cell resolution.
Original languageEnglish
Title of host publication20th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2016)
Place of PublicationSan Diego, CA
Pages377-378
Number of pages2
Publication statusPublished - 9 Oct 2016
Event20th International Conference on Miniaturized Systems for Chemistry and Life Sciences - Convention Center Dublin, Dublin, Ireland
Duration: 9 Oct 201613 Oct 2016
http://www.microtas2016.org/

Conference

Conference20th International Conference on Miniaturized Systems for Chemistry and Life Sciences
Abbreviated titleMicroTas 2016
CountryIreland
CityDublin
Period9/10/1613/10/16
Internet address

Keywords

  • single-cell
  • gold nanorod
  • nano vaccine
  • high throughput assay

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