Projects per year
Abstract
This work presents the successful application of a single-cell microfluidic platform for high-throughput, real-time screening of nanoparticle-cell interactions. Taking vaccine delivery as a proof-of-concept application, ovalbumin-conjugated gold nanorods were produced and controllably delivered to primary dendritic cells within the device. Time-lapse imaging enabled monitoring of hundreds of single-cells during exposure to a range of concentrations of nanoparticle conjugates and simultaneous quantification of specific cellular functions. This integrated system provides throughput and statistical data comparable to that obtained with flow cytometry but also offers a novel approach to determine the dynamics of nanoparticle-cell interactions and nanoparticle-mediated antigen delivery with single-cell resolution.
Original language | English |
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Title of host publication | 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2016) |
Place of Publication | San Diego, CA |
Pages | 377-378 |
Number of pages | 2 |
Publication status | Published - 9 Oct 2016 |
Event | 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences - Convention Center Dublin, Dublin, Ireland Duration: 9 Oct 2016 → 13 Oct 2016 http://www.microtas2016.org/ |
Conference
Conference | 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences |
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Abbreviated title | MicroTas 2016 |
Country | Ireland |
City | Dublin |
Period | 9/10/16 → 13/10/16 |
Internet address |
Keywords
- single-cell
- gold nanorod
- nano vaccine
- high throughput assay
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Projects
- 1 Finished
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Additional Sponsorship - University Of Strathclyde
EPSRC (Engineering and Physical Sciences Research Council)
1/07/11 → 30/06/12
Project: Research