Time course changes to structural, mechanical and material properties of bone in rats after complete spinal cord injury

Jonathan A. Williams; Carmen Huesa; Mikael J. Turunen; James A. Oo; Oskars Radzins; Wilf Gardener; James F.C. Windmill; Hanna Isaksson; K. Elizabeth Tanner; John S. Riddell; Sylvie Coupaud

Time course changes to structural, mechanical and material properties of bone in rats after complete spinal cord injury

Jonathan A. Williams, Carmen Huesa, Mikael J. Turunen, James A. Oo, Oskars Radzins, Wilf Gardener, James F.C. Windmill, Hanna Isaksson, K. Elizabeth Tanner, John S. Riddell, Sylvie Coupaud

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Abstract

Objective: Characterise the spatiotemporal trabecular and cortical bone responses to complete spinal cord injury (SCI) in young rats.
Methods: 8-week-old male Wistar rats received T9-transection SCI and were euthanised 2-, 6-, 10- or 16-weeks post-surgery. Outcome measures were assessed using micro-computed tomography, mechanical testing, serum markers and Fourier-transform infrared spectroscopy.
Results: The trabecular and cortical bone responses to SCI are site-specific. Metaphyseal trabecular BV/TV was 59% lower, characterised by fewer and thinner trabeculae at 2-weeks post-SCI, while epiphyseal BV/TV was 23% lower with maintained connectivity. At later-time points, metaphyseal BV/TV remained unchanged, while epiphyseal BV/TV increased. The total area of metaphyseal and mid-diaphyseal cortical bone were lower from 2-weeks and between 6- and 10-weeks post-SCI, respectively. This suggested that SCI-induced bone changes observed in the rat model were not solely attributable to bone loss, but also to suppressed bone growth. No tissue mineral density differences were observed at any time-point, suggesting that decreased whole-bone mechanical properties were primarily the result of changes to the spatial distribution of bone.
Conclusion: Young SCI rat trabecular bone changes resemble those observed clinically in adult and paediatric SCI, while cortical bone changes resemble paediatric SCI only.

Original language	English
Pages (from-to)	212-234
Number of pages	23
Journal	The Journal of Musculoskeletal and Neuronal Interactions
Volume	22
Issue number	2
Publication status	Published - 1 Jun 2022

Keywords

spinal cord injury
rats
time course changes
spinal cord transection
osteoporosis
microCT
bone mechanical properties
Fourier-transform infrared spectroscopy

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Williams-etal-JMNI-2022-Time-course-changes-to-structural mechanical-and-material-properties-of-bone-in-rats-after-complete-spinal-cord-injuryFinal published version, 2.17 MBLicence: CC BY-NC-SA 4.0

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@article{2fbfe4e9821a4b98837a203b3b6fccb9,

title = "Time course changes to structural, mechanical and material properties of bone in rats after complete spinal cord injury",

abstract = "Objective: Characterise the spatiotemporal trabecular and cortical bone responses to complete spinal cord injury (SCI) in young rats. Methods: 8-week-old male Wistar rats received T9-transection SCI and were euthanised 2-, 6-, 10- or 16-weeks post-surgery. Outcome measures were assessed using micro-computed tomography, mechanical testing, serum markers and Fourier-transform infrared spectroscopy.Results: The trabecular and cortical bone responses to SCI are site-specific. Metaphyseal trabecular BV/TV was 59% lower, characterised by fewer and thinner trabeculae at 2-weeks post-SCI, while epiphyseal BV/TV was 23% lower with maintained connectivity. At later-time points, metaphyseal BV/TV remained unchanged, while epiphyseal BV/TV increased. The total area of metaphyseal and mid-diaphyseal cortical bone were lower from 2-weeks and between 6- and 10-weeks post-SCI, respectively. This suggested that SCI-induced bone changes observed in the rat model were not solely attributable to bone loss, but also to suppressed bone growth. No tissue mineral density differences were observed at any time-point, suggesting that decreased whole-bone mechanical properties were primarily the result of changes to the spatial distribution of bone. Conclusion: Young SCI rat trabecular bone changes resemble those observed clinically in adult and paediatric SCI, while cortical bone changes resemble paediatric SCI only.",

keywords = "spinal cord injury, rats, time course changes, spinal cord transection, osteoporosis, microCT, bone mechanical properties, Fourier-transform infrared spectroscopy",

author = "Williams, {Jonathan A.} and Carmen Huesa and Turunen, {Mikael J.} and Oo, {James A.} and Oskars Radzins and Wilf Gardener and Windmill, {James F.C.} and Hanna Isaksson and Tanner, {K. Elizabeth} and Riddell, {John S.} and Sylvie Coupaud",

year = "2022",

month = jun,

day = "1",

language = "English",

volume = "22",

pages = "212--234",

journal = "The Journal of Musculoskeletal and Neuronal Interactions",

issn = "1108-7161",

publisher = "International Society of Musculoskeletal and Neuronal Interactions",

number = "2",

}

TY - JOUR

T1 - Time course changes to structural, mechanical and material properties of bone in rats after complete spinal cord injury

AU - Williams, Jonathan A.

AU - Huesa, Carmen

AU - Turunen, Mikael J.

AU - Oo, James A.

AU - Radzins, Oskars

AU - Gardener, Wilf

AU - Windmill, James F.C.

AU - Isaksson, Hanna

AU - Tanner, K. Elizabeth

AU - Riddell, John S.

AU - Coupaud, Sylvie

PY - 2022/6/1

Y1 - 2022/6/1

N2 - Objective: Characterise the spatiotemporal trabecular and cortical bone responses to complete spinal cord injury (SCI) in young rats. Methods: 8-week-old male Wistar rats received T9-transection SCI and were euthanised 2-, 6-, 10- or 16-weeks post-surgery. Outcome measures were assessed using micro-computed tomography, mechanical testing, serum markers and Fourier-transform infrared spectroscopy.Results: The trabecular and cortical bone responses to SCI are site-specific. Metaphyseal trabecular BV/TV was 59% lower, characterised by fewer and thinner trabeculae at 2-weeks post-SCI, while epiphyseal BV/TV was 23% lower with maintained connectivity. At later-time points, metaphyseal BV/TV remained unchanged, while epiphyseal BV/TV increased. The total area of metaphyseal and mid-diaphyseal cortical bone were lower from 2-weeks and between 6- and 10-weeks post-SCI, respectively. This suggested that SCI-induced bone changes observed in the rat model were not solely attributable to bone loss, but also to suppressed bone growth. No tissue mineral density differences were observed at any time-point, suggesting that decreased whole-bone mechanical properties were primarily the result of changes to the spatial distribution of bone. Conclusion: Young SCI rat trabecular bone changes resemble those observed clinically in adult and paediatric SCI, while cortical bone changes resemble paediatric SCI only.

AB - Objective: Characterise the spatiotemporal trabecular and cortical bone responses to complete spinal cord injury (SCI) in young rats. Methods: 8-week-old male Wistar rats received T9-transection SCI and were euthanised 2-, 6-, 10- or 16-weeks post-surgery. Outcome measures were assessed using micro-computed tomography, mechanical testing, serum markers and Fourier-transform infrared spectroscopy.Results: The trabecular and cortical bone responses to SCI are site-specific. Metaphyseal trabecular BV/TV was 59% lower, characterised by fewer and thinner trabeculae at 2-weeks post-SCI, while epiphyseal BV/TV was 23% lower with maintained connectivity. At later-time points, metaphyseal BV/TV remained unchanged, while epiphyseal BV/TV increased. The total area of metaphyseal and mid-diaphyseal cortical bone were lower from 2-weeks and between 6- and 10-weeks post-SCI, respectively. This suggested that SCI-induced bone changes observed in the rat model were not solely attributable to bone loss, but also to suppressed bone growth. No tissue mineral density differences were observed at any time-point, suggesting that decreased whole-bone mechanical properties were primarily the result of changes to the spatial distribution of bone. Conclusion: Young SCI rat trabecular bone changes resemble those observed clinically in adult and paediatric SCI, while cortical bone changes resemble paediatric SCI only.

KW - spinal cord injury

KW - rats

KW - time course changes

KW - spinal cord transection

KW - osteoporosis

KW - microCT

KW - bone mechanical properties

KW - Fourier-transform infrared spectroscopy

UR - http://www.ismni.org/jmni/index.php

M3 - Article

SN - 1108-7161

VL - 22

SP - 212

EP - 234

JO - The Journal of Musculoskeletal and Neuronal Interactions

JF - The Journal of Musculoskeletal and Neuronal Interactions

IS - 2

ER -

Time course changes to structural, mechanical and material properties of bone in rats after complete spinal cord injury

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Soft And Small: Acoustic Transducers Inspired By Nature - SASATIN (EU European Research Council (ERC) Consolidator Grant) | Cosentino, Melania

Medical Devices Doctoral Training Centre Renewal | Williams, Jonathan

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Time course changes to structural, mechanical and material properties of bone in rats after complete spinal cord injury

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Soft And Small: Acoustic Transducers Inspired By Nature - SASATIN (EU European Research Council (ERC) Consolidator Grant) | Cosentino, Melania

Medical Devices Doctoral Training Centre Renewal | Williams, Jonathan

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