TY - JOUR
T1 - Time-correlated single-photon counting fluorescence lifetime confocal imaging of decayed and sound dental structures with a white-light supercontinuum source
AU - McConnell, G.
AU - Girkin, J.M.
AU - Ameer-Beg, S.M.
AU - Barber, P.R.
AU - Banerjee, B.V.A.
AU - Watson, D.T.
AU - Cook, R.
PY - 2007/2
Y1 - 2007/2
N2 - We report the demonstration of time-correlated single-photon
counting (TCSPC) fluorescence lifetime imaging (FLIM) to ex
vivo decayed and healthy dentinal tooth structures, using a
white-light supercontinuumexcitation source. By using a 100
fs-pulsed Ti:Sapphire laser with a low-frequency chirp topump
a 30-cm long section of photonic crystal fibre, a ps-pulsed
white-light supercontinuum was created. Optical bandpass
interference filters were then applied to this broad-bandwidth
source to select the 488-nm excitation wavelength required
to perform TCSPC FLIM of dental structures. Decayed dentine
showed significantly shorter lifetimes, discriminating it from
healthy tissue and hard, stained and thus affected but noninfected
material. The white-light generation source provides
a flexible method of producing variable-bandwidth visible and
ps-pulsed light for TCSPC FLIM. The results from the dental
tissue indicate a potential method of discriminating diseased
tissue from sound, but stained tissue,which could be of crucial
importance in limiting tissue resection during preparation for
clinical restorations.
AB - We report the demonstration of time-correlated single-photon
counting (TCSPC) fluorescence lifetime imaging (FLIM) to ex
vivo decayed and healthy dentinal tooth structures, using a
white-light supercontinuumexcitation source. By using a 100
fs-pulsed Ti:Sapphire laser with a low-frequency chirp topump
a 30-cm long section of photonic crystal fibre, a ps-pulsed
white-light supercontinuum was created. Optical bandpass
interference filters were then applied to this broad-bandwidth
source to select the 488-nm excitation wavelength required
to perform TCSPC FLIM of dental structures. Decayed dentine
showed significantly shorter lifetimes, discriminating it from
healthy tissue and hard, stained and thus affected but noninfected
material. The white-light generation source provides
a flexible method of producing variable-bandwidth visible and
ps-pulsed light for TCSPC FLIM. The results from the dental
tissue indicate a potential method of discriminating diseased
tissue from sound, but stained tissue,which could be of crucial
importance in limiting tissue resection during preparation for
clinical restorations.
KW - PACS
KW - 42.62.Be Biological and medical applications
KW - 42.65.-k nonlinear optics
KW - 87.64.Ni optical absorption
KW - magnetic circular dichroism
KW - fluorescence spectroscopy
UR - http://onlinelibrary.wiley.com/journal/10.1111/(ISSN)1365-2818
UR - http://dx.doi.org/10.1111/j.1365-2818.2007.01724.x
U2 - 10.1111/j.1365-2818.2007.01724.x
DO - 10.1111/j.1365-2818.2007.01724.x
M3 - Article
VL - 225
SP - 126
EP - 136
JO - Journal of Microscopy
JF - Journal of Microscopy
SN - 0022-2720
IS - 2
ER -