Tightly anchored tissue-mimetic matrices as instructive stem cell microenvironments

Marina Prewitz, F Philipp Seib, Malte von Bonin, Jens Friedrichs, Aline Stißel, Christian Niehage, Katrin Müller, Konstantinos Anastassiadis, Claudia Waskow, Bernard Hoflack, Martin Bornhäuser, Carsten Werner

Research output: Contribution to journalArticle

111 Citations (Scopus)

Abstract

A major obstacle in defining the exact role of extracellular matrix (ECM) in stem cell niches is the lack of suitable in vitro methods that recapitulate complex ECM microenvironments. Here we describe a methodology that permits reliable anchorage of native cell–secreted ECM to culture carriers. We validated our approach by fabricating two types of human bone marrow–specific ECM substrates that were robust enough to support human mesenchymal stem cells (MSCs) and hematopoietic stem and progenitor cells in vitro. We characterized the molecular composition, structural features and nanomechanical properties of the MSC-derived ECM preparations and demonstrated their ability to support expansion and differentiation of bone marrow stem cells. Our methodology enables the deciphering and modulation of native-like multicomponent ECMs of tissue-resident stem cells and will therefore prepare the ground for a more rational design of engineered stem cell niches.
Original languageEnglish
Pages (from-to)788–794
Number of pages7
JournalNature Methods
Volume10
Issue number8
Early online date23 Jun 2013
DOIs
Publication statusPublished - 2013

Fingerprint

Stem Cell Niche
Stem cells
Extracellular Matrix
Tissue
Hematopoietic Stem Cells
Mesenchymal Stromal Cells
Stem Cells
Bone
Military electronic countermeasures
Bone Marrow Cells
Bone and Bones
Modulation
Substrates
Chemical analysis

Keywords

  • stem cell niches
  • microenvironments
  • human bone marrow

Cite this

Prewitz, M., Seib, F. P., von Bonin, M., Friedrichs, J., Stißel, A., Niehage, C., ... Werner, C. (2013). Tightly anchored tissue-mimetic matrices as instructive stem cell microenvironments. Nature Methods , 10(8), 788–794. https://doi.org/10.1038/nmeth.2523
Prewitz, Marina ; Seib, F Philipp ; von Bonin, Malte ; Friedrichs, Jens ; Stißel, Aline ; Niehage, Christian ; Müller, Katrin ; Anastassiadis, Konstantinos ; Waskow, Claudia ; Hoflack, Bernard ; Bornhäuser, Martin ; Werner, Carsten. / Tightly anchored tissue-mimetic matrices as instructive stem cell microenvironments. In: Nature Methods . 2013 ; Vol. 10, No. 8. pp. 788–794.
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Prewitz, M, Seib, FP, von Bonin, M, Friedrichs, J, Stißel, A, Niehage, C, Müller, K, Anastassiadis, K, Waskow, C, Hoflack, B, Bornhäuser, M & Werner, C 2013, 'Tightly anchored tissue-mimetic matrices as instructive stem cell microenvironments', Nature Methods , vol. 10, no. 8, pp. 788–794. https://doi.org/10.1038/nmeth.2523

Tightly anchored tissue-mimetic matrices as instructive stem cell microenvironments. / Prewitz, Marina; Seib, F Philipp; von Bonin, Malte; Friedrichs, Jens; Stißel, Aline; Niehage, Christian; Müller, Katrin; Anastassiadis, Konstantinos; Waskow, Claudia; Hoflack, Bernard; Bornhäuser, Martin; Werner, Carsten.

In: Nature Methods , Vol. 10, No. 8, 2013, p. 788–794.

Research output: Contribution to journalArticle

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AU - Prewitz, Marina

AU - Seib, F Philipp

AU - von Bonin, Malte

AU - Friedrichs, Jens

AU - Stißel, Aline

AU - Niehage, Christian

AU - Müller, Katrin

AU - Anastassiadis, Konstantinos

AU - Waskow, Claudia

AU - Hoflack, Bernard

AU - Bornhäuser, Martin

AU - Werner, Carsten

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