Three-dimensional STED microscopy of aberrating tissue using dual adaptive optics

Brian R. Patton; Daniel Burke; David Owald; Travis J. Gould; Joerg Bewersdorf; Martin J. Booth

doi:10.1364/OE.24.008862

Three-dimensional STED microscopy of aberrating tissue using dual adaptive optics

Brian R. Patton, Daniel Burke, David Owald, Travis J. Gould, Joerg Bewersdorf, Martin J. Booth

Physics

Research output: Contribution to journal › Article › peer-review

73 Citations (Scopus)

29 Downloads (Pure)

Abstract

When imaging through tissue, the optical inhomogeneities of the sample generate aberrations that can prevent effective Stimulated Emission Depletion (STED) imaging. This is particularly problematic for 3D-enhanced STED. We present here an adaptive optics implementation that incorporates two adaptive optic elements to enable correction in all beam paths, allowing performance improvement in thick tissue samples. We use this to demonstrate 3D STED imaging of complex structures in Drosophila melanogaster brains.

Original language	English
Pages (from-to)	8862-8876
Number of pages	15
Journal	Optics Express
Volume	24
Issue number	8
DOIs	https://doi.org/10.1364/OE.24.008862
Publication status	Published - 13 Apr 2016

Keywords

superresolution
STED microscopy
scanning microscopy
adaptive optics

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10.1364/OE.24.008862Licence: CC BY 4.0

Patton-etal-OE-2016-Three-dimensional-STED-microscopy-of-aberrating-tissueFinal published version, 4.26 MBLicence: CC BY 4.0

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AU - Booth, Martin J.

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Three-dimensional STED microscopy of aberrating tissue using dual adaptive optics

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Brian Patton

Imaging Deep Tissue Neural Processes with Nanodiamond (URF)

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Three-dimensional STED microscopy of aberrating tissue using dual adaptive optics

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Brian Patton

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Imaging Deep Tissue Neural Processes with Nanodiamond (URF)

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