Three-dimensional STED microscopy of aberrating tissue using dual adaptive optics

Brian R. Patton, Daniel Burke, David Owald, Travis J. Gould, Joerg Bewersdorf, Martin J. Booth

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Abstract

When imaging through tissue, the optical inhomogeneities of the sample generate aberrations that can prevent effective Stimulated Emission Depletion (STED) imaging. This is particularly problematic for 3D-enhanced STED. We present here an adaptive optics implementation that incorporates two adaptive optic elements to enable correction in all beam paths, allowing performance improvement in thick tissue samples. We use this to demonstrate 3D STED imaging of complex structures in Drosophila melanogaster brains.

Original languageEnglish
Pages (from-to)8862-8876
Number of pages15
JournalOptics Express
Volume24
Issue number8
DOIs
Publication statusPublished - 13 Apr 2016

Keywords

  • superresolution
  • STED microscopy
  • scanning microscopy
  • adaptive optics

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Patton, B. R., Burke, D., Owald, D., Gould, T. J., Bewersdorf, J., & Booth, M. J. (2016). Three-dimensional STED microscopy of aberrating tissue using dual adaptive optics. Optics Express, 24(8), 8862-8876. https://doi.org/10.1364/OE.24.008862