This histamine-like effects of Phallusia nigra extract: evidences for direct activity at H1 receptors

L.V. Costa, E.L.A. Malpezzi, R.G.S. Berlinck, E.G. Rowan, J.C. deFreitas

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

The methanol extract (mol. wt lower than 3,000 Da) of the sea squirt Phallusia nigra has stimulatory activity on guinea-pig ileum preparations. This effect was inhibited by cyproheptadine and mepyramine, but not by atropine. Mepyramine antagonized competitively the extract activity with a pA2 of 10.09 ± 1.12, suggesting a direct activity on H1 histamine receptors. The extract was also assayed on guinea-pig right atria, however, only a mild increase in spontaneous contractions was observed compared to histamine, showing that the extract was a rather poor activator of cardiac H2 receptors. Histamine was not detected upon TLC analysis of the extract by comparison with an authentic standard.
LanguageEnglish
Pages111-115
Number of pages4
JournalComparative biochemistry and physiology. Part C, Pharmacology, toxicology & endocrinology
Volume117
Issue number1
DOIs
Publication statusPublished - May 1997

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Histamine Agents
Pyrilamine
Histamine H1 Receptors
Histamine
Guinea Pigs
Cyproheptadine
Histamine H2 Receptors
Heart Atria
Atropine
Ileum
Oceans and Seas
Methanol

Keywords

  • Phallusia nigra
  • tunicates
  • histamine agonist
  • histamine receptors
  • mepyramine
  • competitive antagonism
  • guinea pig ileum
  • guinea pig right atria

Cite this

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title = "This histamine-like effects of Phallusia nigra extract: evidences for direct activity at H1 receptors",
abstract = "The methanol extract (mol. wt lower than 3,000 Da) of the sea squirt Phallusia nigra has stimulatory activity on guinea-pig ileum preparations. This effect was inhibited by cyproheptadine and mepyramine, but not by atropine. Mepyramine antagonized competitively the extract activity with a pA2 of 10.09 ± 1.12, suggesting a direct activity on H1 histamine receptors. The extract was also assayed on guinea-pig right atria, however, only a mild increase in spontaneous contractions was observed compared to histamine, showing that the extract was a rather poor activator of cardiac H2 receptors. Histamine was not detected upon TLC analysis of the extract by comparison with an authentic standard.",
keywords = "Phallusia nigra, tunicates, histamine agonist, histamine receptors, mepyramine, competitive antagonism, guinea pig ileum, guinea pig right atria",
author = "L.V. Costa and E.L.A. Malpezzi and R.G.S. Berlinck and E.G. Rowan and J.C. deFreitas",
year = "1997",
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doi = "10.1016/S0742-8413(96)00228-9",
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journal = "Comparative biochemistry and physiology. Part C, Pharmacology, toxicology & endocrinology",
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}

This histamine-like effects of Phallusia nigra extract: evidences for direct activity at H1 receptors. / Costa, L.V.; Malpezzi, E.L.A.; Berlinck, R.G.S.; Rowan, E.G.; deFreitas, J.C.

In: Comparative biochemistry and physiology. Part C, Pharmacology, toxicology & endocrinology, Vol. 117, No. 1, 05.1997, p. 111-115.

Research output: Contribution to journalArticle

TY - JOUR

T1 - This histamine-like effects of Phallusia nigra extract: evidences for direct activity at H1 receptors

AU - Costa, L.V.

AU - Malpezzi, E.L.A.

AU - Berlinck, R.G.S.

AU - Rowan, E.G.

AU - deFreitas, J.C.

PY - 1997/5

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AB - The methanol extract (mol. wt lower than 3,000 Da) of the sea squirt Phallusia nigra has stimulatory activity on guinea-pig ileum preparations. This effect was inhibited by cyproheptadine and mepyramine, but not by atropine. Mepyramine antagonized competitively the extract activity with a pA2 of 10.09 ± 1.12, suggesting a direct activity on H1 histamine receptors. The extract was also assayed on guinea-pig right atria, however, only a mild increase in spontaneous contractions was observed compared to histamine, showing that the extract was a rather poor activator of cardiac H2 receptors. Histamine was not detected upon TLC analysis of the extract by comparison with an authentic standard.

KW - Phallusia nigra

KW - tunicates

KW - histamine agonist

KW - histamine receptors

KW - mepyramine

KW - competitive antagonism

KW - guinea pig ileum

KW - guinea pig right atria

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JO - Comparative biochemistry and physiology. Part C, Pharmacology, toxicology & endocrinology

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